Icariin induces irisin/FNDC5 expression in C2C12 cells via the AMPK pathway.
In: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jg. 115 (2019-07-01), S. 108930
Online
academicJournal
Zugriff:
Backgroud Icariin, a major bioactive pharmaceutical component of the Chinese herbal medicine Epimedii Herba, has demonstrated lipid-lowering and anti-obesity effects. Irisin/ fibronectin type III domain-containing 5 (FNDC5) protects against obesity by inducing browning in white adipose tissue. Objectives This study investigated the effects of icariin on irisin/FNDC5 expression in C2C12 myotubes. Method Cultured murine C2C12 myocytes were used to study the effects of icariin on irisin/FNDC5 expressions by Western-blot, qPCR, Elisa and Immunofluorescence. We also investigated FNDC5 expression in icariin-treated intact mice. Results Icariin increased irisin/FNDC5 protein levels. mRNA levels of irisin/FNDC5 were also increased in C2C12 myocytes after treatment with icariin. Icariin increased peroxisome proliferator-activated receptor gamma co-activator 1alpha (PGC-1α) protein and mRNA levels. Additionally, icariin exposure resulted in phosphorylation of AMP-activated protein kinase (AMPK) in a dose-dependent manner. The regulatory effect of icariin on FNDC5 protein expression was blocked by the AMPK antagonist compound C or silencing of AMPK, suggesting that icariin increased FNDC5 protein expression via the AMPK pathway. In vivo, icariin decreased body weight gain in C57BL/6 mice and increased FNDC5, PGC-1α, and p-AMPK expression levels in skeletal muscle. Conclusions Taken together, our results indicated that icariin induces irisin/FNDC5 expression via the AMPK pathway, indicating that icariin may be promising as an anti-obesity drug.
(Copyright © 2019 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
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Icariin induces irisin/FNDC5 expression in C2C12 cells via the AMPK pathway.
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Autor/in / Beteiligte Person: | Chen, SQ ; Ding, LN ; Zeng, NX ; Liu, HM ; Zheng, SH ; Xu, JW ; Li, RM |
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Zeitschrift: | Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jg. 115 (2019-07-01), S. 108930 |
Veröffentlichung: | Paris : Editions Scientifiques Elsevier ; <i>Original Publication</i>: New York, N.Y. : Masson Pub. USA, Inc., c1982-, 2019 |
Medientyp: | academicJournal |
ISSN: | 1950-6007 (electronic) |
DOI: | 10.1016/j.biopha.2019.108930 |
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