Einzeltreffer — DigiBib

Purpose: Icariin (ICA) is one of the main active constituents of Herba Epimedii for improving osteogenesis. It is necessary to create a simple and efficient method to load ICA onto the surface of titanium (Ti) implant. The purpose of this study was to establish a local ICA delivery system via a layer-by-layer (LbL) self-assembly system on phase-transited lysozyme (PTL)-primed Ti surface.

Materials and Methods: A PTL nanofilm was first firmly coated on the pristine Ti. Then, the ICA-loaded hyaluronic acid/chitosan (HA/CS) multilayer was applied via the LbL system to form the HA/CS-ICA surface. This established HA/CS-ICA surface was characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle measurement. The ICA release pattern of the HA/CS-ICA surface was also examined. MC3T3-E1 osteoblast culture test and a rat model were used to evaluate the effects of the HA/CS-ICA surface in vitro and in vivo.

Results: SEM, XPS and contact angle measurement demonstrated successful fabrication of the HA/CS-ICA surface. The HA/CS-ICA surfaces with different ICA concentrations revealed a controlled release profile of ICA during a 2-week monitoring span. Osteoblasts grown on the coated substrates displayed higher adhesion, viability, proliferation and ALP activity than those on the polished Ti surface. Furthermore, in vivo histological evaluation revealed much obvious bone formation in the ICA-coated group by histological staining and double fluorescent labeling at 2 weeks after implantation.

Conclusion: The present study demonstrated that ICA-immobilized HA/CS multilayer on the PTL-primed Ti surface had a sustained release pattern of ICA which could promote the osteogenesis of osteoblasts in vitro and improve early osseointegration in vivo. This study provides a novel method for creating a sustained ICA delivery system to improve osteoblast response and osseointegration.

Competing Interests: Disclosure The authors report no conflicts of interest in this work.

Titel:	Loading icariin on titanium surfaces by phase-transited lysozyme priming and layer-by-layer self-assembly of hyaluronic acid/chitosan to improve surface osteogenesis ability.
Autor/in / Beteiligte Person:	Song, Y ; Ma, A ; Ning, J ; Zhong, X ; Zhang, Q ; Zhang, X ; Hong, G ; Li, Y ; Sasaki, K ; Li, C
Link:	Zum Volltext
Zeitschrift:	International journal of nanomedicine, Jg. 13 (2018-10-23), S. 6751
Veröffentlichung:	Auckland : DOVE Medical Press,, 2018
Medientyp:	academicJournal
ISSN:	1178-2013 (electronic)
DOI:	10.2147/IJN.S174953
Schlagwort:	Animals Flavonoids chemistry Male Osteoblasts drug effects Osteoblasts metabolism Rats Rats, Sprague-Dawley Surface Properties Chitosan chemistry Flavonoids pharmacology Hyaluronic Acid chemistry Muramidase chemistry Osteoblasts cytology Osteogenesis drug effects Titanium chemistry
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Int J Nanomedicine] 2018 Oct 23; Vol. 13, pp. 6751-6767. <i>Date of Electronic Publication: </i>2018 Oct 23 (<i>Print Publication: </i>2018). MeSH Terms: Chitosan / chemistry ; Flavonoids / pharmacology ; Hyaluronic Acid / chemistry ; Muramidase / chemistry ; Osteoblasts / cytology ; Osteogenesis / drug effects ; Titanium / *chemistry ; Animals ; Flavonoids / chemistry ; Male ; Osteoblasts / drug effects ; Osteoblasts / metabolism ; Rats ; Rats, Sprague-Dawley ; Surface Properties References: Sci Rep. 2016 Nov 08;6:36408. (PMID: 27821857) ; ACS Appl Mater Interfaces. 2015 Jul 8;7(26):14384-96. (PMID: 26076385) ; Arch Osteoporos. 2016 Dec;11(1):29. (PMID: 27637755) ; Phytomedicine. 2010 May;17(6):414-23. (PMID: 19747809) ; J Mater Sci Mater Med. 2011 Feb;22(2):371-9. (PMID: 21140197) ; Molecules. 2013 Dec 24;19(1):177-203. (PMID: 24368601) ; Stem Cells Transl Med. 2014 Apr;3(4):530-40. (PMID: 24558162) ; J Ethnopharmacol. 2013 Feb 13;145(3):715-21. (PMID: 23261485) ; Neurosurg Focus. 2001 Apr 15;10(4):E1. (PMID: 16732625) ; Mater Sci Eng C Mater Biol Appl. 2015 Jan;46:148-57. (PMID: 25491971) ; Int J Nanomedicine. 2018 Apr 11;13:2189-2197. (PMID: 29692613) ; Int J Nanomedicine. 2016 Apr 01;11:1367-82. (PMID: 27099496) ; Tissue Eng Part B Rev. 2013 Jun;19(3):233-53. (PMID: 23088597) ; Biomatter. 2012 Jul-Sep;2(3):149-57. (PMID: 23507866) ; Neurol Sci. 2013 Jun;34(6):941-7. (PMID: 22833221) ; Int J Pharm. 2016 Dec 30;515(1-2):176-185. (PMID: 27713029) ; Biomaterials. 2010 Mar;31(8):2348-57. (PMID: 20004967) ; Tissue Eng Part A. 2010 Jan;16(1):233-43. (PMID: 19698057) ; Eur J Pharmacol. 2013 Aug 15;714(1-3):15-22. (PMID: 23764463) ; J Clin Periodontol. 2014 Feb;41(2):181-90. (PMID: 24256613) ; J Appl Oral Sci. 2015 May-Jun;23(3):279-87. (PMID: 26221922) ; Int J Mol Med. 2012 Mar;29(3):435-9. (PMID: 22109711) ; Mater Sci Eng C Mater Biol Appl. 2018 Nov 1;92:206-215. (PMID: 30184744) ; Biomark Res. 2016 Dec 14;4:24. (PMID: 27999672) ; Bone. 2009 Feb;44(2):225-32. (PMID: 19056525) ; Science. 2009 Jun 26;324(5935):1673-7. (PMID: 19556500) ; Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:399-409. (PMID: 28629034) ; Integr Biol (Camb). 2015 Feb;7(2):250-62. (PMID: 25578396) ; Phytomedicine. 2005 Mar;12(3):189-93. (PMID: 15830840) ; Mater Sci Eng C Mater Biol Appl. 2017 Feb 1;71:260-267. (PMID: 27987707) ; Biomaterials. 2011 Feb;32(6):1495-507. (PMID: 21122912) ; Biomaterials. 2011 Feb;32(5):1446-53. (PMID: 21084117) ; Sci Rep. 2017 Jul 11;7(1):5077. (PMID: 28698566) ; J Adv Prosthodont. 2016 Jun;8(3):235-40. (PMID: 27350860) ; ACS Appl Mater Interfaces. 2018 Feb 14;10(6):5967-5977. (PMID: 29338177) ; J Leukoc Biol. 2011 May;89(5):669-73. (PMID: 21208896) ; Angew Chem Int Ed Engl. 2009;48(47):8974-7. (PMID: 19847838) ; Evid Based Complement Alternat Med. 2013;2013:652317. (PMID: 24348713) ; Colloids Surf B Biointerfaces. 2015 Oct 1;134:401-7. (PMID: 26218713) ; Biomaterials. 2006 Apr;27(11):2450-67. (PMID: 16337266) ; Acta Biomater. 2014 Feb;10(2):557-79. (PMID: 24211734) ; Biomaterials. 2015 Jan;36:44-54. (PMID: 25308520) ; Acta Biomater. 2012 Jan;8(1):439-48. (PMID: 22040682) ; ACS Appl Mater Interfaces. 2016 Dec 14;8(49):33972-33981. (PMID: 27960367) ; Biomaterials. 2018 Jan;153:1-13. (PMID: 29096397) ; PLoS One. 2016 Jan 19;11(1):e0146957. (PMID: 26783746) ; Cell Physiol Biochem. 2017;41(4):1605-1615. (PMID: 28355606) ; Differentiation. 2003 Dec;71(9-10):567-77. (PMID: 14686954) ; Connect Tissue Res. 2013;54(4-5):290-6. (PMID: 23971976) ; Int J Nanomedicine. 2017 Oct 19;12:7721-7735. (PMID: 29089766) ; Int J Nanomedicine. 2017 Feb 20;12:1415-1430. (PMID: 28260888) ; J Control Release. 2002 Jan 17;78(1-3):187-97. (PMID: 11772460) ; Acta Biomater. 2010 Mar;6(3):776-85. (PMID: 19703596) ; Biochem Biophys Res Commun. 2008 May 2;369(2):444-8. (PMID: 18295595) ; Biomaterials. 2013 May;34(16):4027-37. (PMID: 23465831) ; Semin Immunol. 2008 Apr;20(2):86-100. (PMID: 18162407) ; Int J Mol Sci. 2015 May 06;16(5):10324-36. (PMID: 25955650) ; Phytother Res. 2014 Apr;28(4):498-509. (PMID: 23824956) ; Mater Sci Eng C Mater Biol Appl. 2016 Jan 1;58:310-5. (PMID: 26478315) ; J Steroid Biochem Mol Biol. 2014 Sep;143:141-51. (PMID: 24607839) ; Sci Rep. 2017 Aug 17;7(1):8616. (PMID: 28819219) ; Planta Med. 2013 Nov;79(16):1501-8. (PMID: 24072503) ; Bone. 2010 May;46(5):1436-41. (PMID: 20116464) ; J Contemp Dent Pract. 2017 Aug 1;18(8):705-709. (PMID: 28816194) Contributed Indexing: Keywords: controlled release; icariin; layer-by-layer self-assembly; osteogenesis; phase-transited lysozyme; surface modification of titanium Substance Nomenclature: 0 (Flavonoids) ; 9004-61-9 (Hyaluronic Acid) ; 9012-76-4 (Chitosan) ; D1JT611TNE (Titanium) ; EC 3.2.1.17 (Muramidase) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20181115 Date Completed: 20181217 Latest Revision: 20220330 Update Code: 20240513 PubMed Central ID: PMC6204858

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Loading icariin on titanium surfaces by phase-transited lysozyme priming and layer-by-layer self-assembly of hyaluronic acid/chitosan to improve surface osteogenesis ability.