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The pig is one of the main reservoirs of Yersinia enterocolitica strains pathogenic to humans. A description of the Y. enterocolitica population in this reservoir, and accurate discriminatory techniques for typing isolates are needed for prevention, outbreak investigation, and surveillance. This study investigates the genetic diversity of pathogenic Y. enterocolitica isolates obtained from pig tonsils in a French pig slaughterhouse in 2009 (S1) and 2010 (S2). The use of Pulsed-Field Gel Electrophoresis (PFGE) and MLVA as typing techniques was also compared and evaluated. First, a total of 167 isolates (12 of biotype 3 recovered during S1, and 155 of biotype 4 recovered during S1 and S2) were typed by PFGE using the XbaI enzyme. MLVA was then tested on all the biotype 3 isolates in addition to 70 selected biotype 4 isolates recovered over the 2 years. PFGE generated two specific XbaI-PFGE profiles for biotype 3 isolates. Nine XbaI profiles were obtained for biotype 4, with a higher diversity (ID = 0.599) than biotype 3 (ID = 0.167). Two out of the nine XbaI profiles were reported during both surveys and at different months. MLVA improved the differentiation between isolates; the index of diversity reached 0.621 and 0.958, respectively, for biotype 3 (three MLVA types) and biotype 4 (32 MLVA types). The MLVA types for biotype 4 differed over the two surveys, but some isolates with different MLVA types were genetically closely related. This study provides an initial evaluation of the genetic diversity of Y. enterocolitica strains isolated from pigs in France. We show that some PFGE profiles are maintained in the pig production sector, and, through MLVA, that part of the Y. enterocolitica population remained genetically close over the two years. MLVA proved its effectiveness as a tool for investigating pathogenic Y. enterocolitica strains isolated from pigs.

Titel:	Diversity of Yersinia enterocolitica isolated from pigs in a French slaughterhouse over 2 years.
Autor/in / Beteiligte Person:	Raymond, P ; Houard, E ; Denis, M ; Esnault, E
Link:	Zum Volltext
Zeitschrift:	MicrobiologyOpen, Jg. 8 (2019-06-01), Heft 6, S. e00751
Veröffentlichung:	Oxford : Wiley-Blackwell, 2019
Medientyp:	academicJournal
ISSN:	2045-8827 (electronic)
DOI:	10.1002/mbo3.751
Schlagwort:	Abattoirs statistics & numerical data Animals Bacterial Typing Techniques Electrophoresis, Gel, Pulsed-Field France Phylogeny Yersinia enterocolitica classification Yersinia enterocolitica genetics Disease Reservoirs microbiology Genetic Variation Swine microbiology Yersinia enterocolitica isolation & purification
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Microbiologyopen] 2019 Jun; Vol. 8 (6), pp. e00751. <i>Date of Electronic Publication: </i>2018 Oct 22. MeSH Terms: Genetic Variation* ; Disease Reservoirs / microbiology ; Swine / microbiology ; Yersinia enterocolitica / *isolation & purification ; Abattoirs / statistics & numerical data ; Animals ; Bacterial Typing Techniques ; Electrophoresis, Gel, Pulsed-Field ; France ; Phylogeny ; Yersinia enterocolitica / classification ; Yersinia enterocolitica / genetics References: Foodborne Pathog Dis. 2011 Mar;8(3):445-50. (PMID: 20187744) ; Food Microbiol. 2010 Feb;27(1):158-61. (PMID: 19913707) ; Appl Environ Microbiol. 2012 Apr;78(8):2949-56. (PMID: 22327599) ; J Med Microbiol. 2006 Nov;55(Pt 11):1539-1548. (PMID: 17030914) ; J Microbiol Methods. 2010 Nov;83(2):244-9. (PMID: 20850483) ; Microbiologyopen. 2019 Jun;8(6):e00751. (PMID: 30350482) ; Lett Appl Microbiol. 2010 Apr;50(4):412-8. (PMID: 20158605) ; PLoS Negl Trop Dis. 2017 Jan 12;11(1):e0005216. (PMID: 28081123) ; J Clin Microbiol. 1988 Nov;26(11):2465-6. (PMID: 3069867) ; J Clin Microbiol. 2007 Aug;45(8):2508-15. (PMID: 17553973) ; Appl Environ Microbiol. 1994 Dec;60(12):4371-9. (PMID: 7811077) ; Emerg Infect Dis. 2017 Sep;23(9):1486-1492. (PMID: 28820137) ; Int J Infect Dis. 2015 May;34:76-8. (PMID: 25800947) ; J Food Prot. 2000 Jan;63(1):31-5. (PMID: 10643766) ; Foodborne Pathog Dis. 2016 Jun;13(6):289-95. (PMID: 26990517) ; J Clin Microbiol. 2011 Nov;49(11):3997-4000. (PMID: 21918028) ; Int J Food Microbiol. 2014 May 2;177:9-15. (PMID: 24598512) ; Foodborne Pathog Dis. 2014 Aug;11(8):596-601. (PMID: 24823493) ; Int J Food Microbiol. 2015 Jul 2;204:33-40. (PMID: 25835198) ; J Clin Microbiol. 2006 Jul;44(7):2524-32. (PMID: 16825375) ; Foodborne Pathog Dis. 2012 Mar;9(3):179-89. (PMID: 22217012) ; Int J Infect Dis. 2016 May;46:1-7. (PMID: 26987478) ; Lett Appl Microbiol. 1999 Nov;29(5):308-12. (PMID: 10664971) ; Prev Vet Med. 2010 Feb 1;93(2-3):110-20. (PMID: 19926152) ; EFSA J. 2017 Dec 12;15(12):e05077. (PMID: 32625371) ; Syst Appl Microbiol. 2003 Mar;26(1):132-7. (PMID: 12747421) ; Emerg Infect Dis. 2012 Sep;18(9):1496-9. (PMID: 22932318) ; Clin Microbiol Infect. 1996 Aug;2(1):2-11. (PMID: 11866804) ; Int J Food Microbiol. 2007 Nov 1;119(3):207-12. (PMID: 17727997) ; J Clin Microbiol. 1994 Dec;32(12):2913-20. (PMID: 7883877) ; Int J Food Microbiol. 2014 Mar 17;174:56-62. (PMID: 24451473) ; BMC Microbiol. 2011 Feb 25;11:42. (PMID: 21349206) ; Int J Food Microbiol. 2018 Jul 2;276:5-9. (PMID: 29649750) ; Euro Surveill. 2016 Aug 25;21(34):. (PMID: 27588690) ; PLoS One. 2012;7(5):e37309. (PMID: 22615971) ; Zoonoses Public Health. 2014 Sep;61(6):405-10. (PMID: 24237638) ; J Food Prot. 2016 Apr;79(4):677-81. (PMID: 27052875) Contributed Indexing: Keywords: Yersinia enterocolitica; MLVA; PFGE; genotyping; pig; slaughterhouse Entry Date(s): Date Created: 20181024 Date Completed: 20200623 Latest Revision: 20231012 Update Code: 20240513 PubMed Central ID: PMC6562139

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Diversity of Yersinia enterocolitica isolated from pigs in a French slaughterhouse over 2 years.