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The rapid and accurate detection of carbapenemase-producing Enterobacteriaceae (CPE) is necessary for patient management and infection control measures. We compared the performance of the BD Phoenix CPO Detect with that of a homemade Carba NP assay and a modified carbapenem inactivation method (mCIM) by challenging all 3 assays with 190 isolates of Enterobacteriaceae with meropenem MICs of >0.125 mg/liter. A total of 160 isolates produced KPC-, IMI-1-, NDM-, IMP-, and OXA-type carbapenemases, while 30 isolates were negative for carbapenemase production. The sensitivity and specificity were 90.6% (95% confidence interval [CI], 85.0% to 94.7%) and 100.0% (95% CI, 88.4% to 100.0%), respectively, for the Carba NP; 100.0% (95% CI, 97.7% to 100.0%) and 96.7% (95% CI, 82.7% to 99.9%), respectively, for the mCIM; and 89.4% (95% CI, 83.5% to 93.7%) and 66.7% (95% CI, 47.2% to 82.7%), respectively, for the BD Phoenix CPO Detect. In particular, the BD CPO Detect failed to detect a significant number of CPE with IMI-1. While the BD Phoenix CPO Detect is able to classify carbapenemases and is built into routine susceptibility testing with the potential to reduce the time to CPE detection, its low specificity means that a positive result will need confirmatory testing by another method.

Titel:	Diagnostic Accuracy of BD Phoenix CPO Detect for Carbapenemase Production in 190 Enterobacteriaceae Isolates.
Autor/in / Beteiligte Person:	Ong, CH ; Ratnayake, L ; Ang, MLT ; Lin, RTP ; Chan, DSG
Link:	Zum Volltext
Zeitschrift:	Journal of clinical microbiology, Jg. 56 (2018-11-27), Heft 12
Veröffentlichung:	Washington, American Society for Microbiology., 2018
Medientyp:	academicJournal
ISSN:	1098-660X (electronic)
DOI:	10.1128/JCM.01043-18
Schlagwort:	Anti-Bacterial Agents metabolism Anti-Bacterial Agents pharmacology Bacterial Proteins classification Bacterial Proteins metabolism Carbapenem-Resistant Enterobacteriaceae enzymology Carbapenem-Resistant Enterobacteriaceae isolation & purification Carbapenems metabolism Carbapenems pharmacology Enterobacteriaceae isolation & purification Enterobacteriaceae Infections microbiology Humans Microbial Sensitivity Tests Sensitivity and Specificity beta-Lactamases classification beta-Lactamases metabolism Bacterial Proteins biosynthesis Bacteriological Techniques standards Diagnostic Tests, Routine standards Enterobacteriaceae enzymology beta-Lactamases biosynthesis
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Evaluation Study; Journal Article Language: English [J Clin Microbiol] 2018 Nov 27; Vol. 56 (12). <i>Date of Electronic Publication: </i>2018 Nov 27 (<i>Print Publication: </i>2018). MeSH Terms: Bacterial Proteins / biosynthesis ; Bacteriological Techniques / standards ; Diagnostic Tests, Routine / standards ; Enterobacteriaceae / enzymology ; beta-Lactamases / *biosynthesis ; Anti-Bacterial Agents / metabolism ; Anti-Bacterial Agents / pharmacology ; Bacterial Proteins / classification ; Bacterial Proteins / metabolism ; Carbapenem-Resistant Enterobacteriaceae / enzymology ; Carbapenem-Resistant Enterobacteriaceae / isolation & purification ; Carbapenems / metabolism ; Carbapenems / pharmacology ; Enterobacteriaceae / isolation & purification ; Enterobacteriaceae Infections / microbiology ; Humans ; Microbial Sensitivity Tests ; Sensitivity and Specificity ; beta-Lactamases / classification ; beta-Lactamases / metabolism References: J Clin Microbiol. 2017 Dec;55(12):3437-3443. (PMID: 28978681) ; Clin Infect Dis. 2017 Feb 1;64(3):257-264. (PMID: 28013264) ; J Antimicrob Chemother. 2018 Apr 1;73(4):909-915. (PMID: 29365094) ; Clin Infect Dis. 2017 May 15;64(suppl_2):S68-S75. (PMID: 28475792) ; J Clin Microbiol. 2015 Sep;53(9):3003-8. (PMID: 26085619) ; Emerg Infect Dis. 2005 Feb;11(2):260-4. (PMID: 15752444) ; J Antimicrob Chemother. 2015 Nov;70(11):3014-22. (PMID: 26260131) ; J Antimicrob Chemother. 2007 Sep;60(3):470-82. (PMID: 17595289) ; Clin Microbiol Rev. 2012 Oct;25(4):682-707. (PMID: 23034326) ; J Infect Dis. 2017 Feb 15;215(suppl_1):S28-S36. (PMID: 28375512) ; Trends Mol Med. 2012 May;18(5):263-72. (PMID: 22480775) ; Antimicrob Agents Chemother. 1996 Sep;40(9):2080-6. (PMID: 8878585) ; J Clin Microbiol. 2017 Aug;55(8):2321-2333. (PMID: 28381609) ; Clin Microbiol Rev. 2017 Jan;30(1):1-22. (PMID: 27795305) ; Antimicrob Agents Chemother. 1997 Feb;41(2):223-32. (PMID: 9021171) ; Antimicrob Agents Chemother. 2006 Apr;50(4):1610-1. (PMID: 16569898) ; Int J Antimicrob Agents. 2018 Sep;52(3):416-420. (PMID: 29807164) ; J Antimicrob Chemother. 2000 Dec;46(6):895-900. (PMID: 11102406) ; Emerg Infect Dis. 2012 Sep;18(9):1503-7. (PMID: 22932472) Contributed Indexing: Keywords: CPO Detect; Carba NP; Phoenix; carbapenemase; mCIM Substance Nomenclature: 0 (Anti-Bacterial Agents) ; 0 (Bacterial Proteins) ; 0 (Carbapenems) ; EC 3.5.2.6 (beta-Lactamases) ; EC 3.5.2.6 (carbapenemase) Entry Date(s): Date Created: 20180914 Date Completed: 20190906 Latest Revision: 20191210 Update Code: 20240513 PubMed Central ID: PMC6258855

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Diagnostic Accuracy of BD Phoenix CPO Detect for Carbapenemase Production in 190 Enterobacteriaceae Isolates.