DT-Diaphorase Prevents Aminochrome-Induced Lysosome Dysfunction in SH-SY5Y Cells.
In: Neurotoxicity research, Jg. 35 (2019), Heft 1, S. 255-259
Online
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Zugriff:
Aminochrome has been reported to induce lysosomal dysfunction by inhibiting the vacuolar H-type ATPase localized in lysosome membrane. DT-diaphorase has been proposed to prevent aminochrome neurotoxicity but it is unknown whether this enzyme prevents aminochrome-induced lysosomal dysfunction. In the present study, we tested the protective role of DT-diaphorase in lysosomal dysfunction by generating a cell line (SH-SY5YsiNQ7) with a stable expression of a siRNA against DT-diaphorase with only 10% expression of mRNA enzyme. The cells differentiated with retinoic acid and 12-o-tetradecanoylphorbol-13-acetate show a significant increase in the expression of tyrosine hydroxylase, vesicular monoamine transporter-2, and dopamine transporter. The incubation of SH-SY5YsiNQ7 cells with 10 μM aminochrome resulted in a significant decrease of lysosome pH determined by using acridine orange, while aminochrome has no effect on SH-SY5Y cells. These results support the proposed protective role of DT-diaphorase against aminochrome-induced lysosomal dysfunction.
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DT-Diaphorase Prevents Aminochrome-Induced Lysosome Dysfunction in SH-SY5Y Cells.
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| Autor/in / Beteiligte Person: | Meléndez, C ; Muñoz, P ; Segura-Aguilar, J |
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| Zeitschrift: | Neurotoxicity research, Jg. 35 (2019), Heft 1, S. 255-259 |
| Veröffentlichung: | <2009-> : New York : Springer ; <i>Original Publication</i>: [Amsterdam?] : Harwood Academic Publishers,, 2019 |
| Medientyp: | academicJournal |
| ISSN: | 1476-3524 (electronic) |
| DOI: | 10.1007/s12640-018-9953-8 |
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