VIP Regulates Morphology and F-Actin Distribution of Schlemm's Canal in a Chronic Intraocular Pressure Hypertension Model via the VPAC2 Receptor.

Chen, L ; Li, M ; et al.

In: Investigative ophthalmology & visual science, Jg. 59 (2018-06-01), Heft 7, S. 2848

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Purpose: To investigate the roles of vasoactive intestinal peptides (VIPs) in regulating the morphology and F-actin distribution of Schlemm's canal (SC) of rat eyes.

Methods: Chronic intraocular pressure (IOP) hypertension models with episcleral venous cauterization (EVC) were treated with topical VIP or PG99-465 (vasoactive intestinal peptide receptors 2 [VPAC2] antagonist). IOPs were measured with Tono-Pen, and the SC parameters, including the cross-section area, circumference, and length, were statistically evaluated by hematoxylin-eosin and CD31 immunohistochemical staining. Immunofluorescence was performed to detect the distribution of F-actin in the SC. Moreover, the distribution of filamentous actin (F-actin) and globular actin (G-actin) in human umbilical vein endothelial cells (HUVECs) was studied under a pressure system by immunofluorescence and Western blotting.

Results: Increased expressions of VIP and VPAC2 receptors, as well as a disordered distribution of F-actin were found in SC endothelial cells (SCEs) in the EVC model. Moreover, topical VIP maintained the normal distribution of F-actin in SCEs, expanded the collapsed SC, and induced a significant decrease in IOP in the EVC model. In in vitro HUVECs, the F-actin/G-actin ratio increased significantly under stress stimulation for 30 minutes. A total of 50 μM VIP helped maintain the normal F-actin/G-actin ratio of HUVECs against stress stimulation.

Conclusions: VIP regulates the distribution of F-actin in SCEs via the VPAC2 receptor in order to induce a decrease in IOP. VIP may represent a new target for antiglaucoma drugs.

Titel:	VIP Regulates Morphology and F-Actin Distribution of Schlemm's Canal in a Chronic Intraocular Pressure Hypertension Model via the VPAC2 Receptor.
Autor/in / Beteiligte Person:	Chen, L ; Li, M ; Luo, Z ; Yan, X ; Yao, K ; Zhao, Y ; Zhang, H
Link:	Zum Volltext
Zeitschrift:	Investigative ophthalmology & visual science, Jg. 59 (2018-06-01), Heft 7, S. 2848
Veröffentlichung:	Brookline Ma : Association For Research In Vision And Ophthalmology (Arvo) ; <i>Original Publication</i>: St. Louis, Mosby., 2018
Medientyp:	academicJournal
ISSN:	1552-5783 (electronic)
DOI:	10.1167/iovs.17-22688
Schlagwort:	Animals Blotting, Western Chronic Disease Fluorescent Antibody Technique, Indirect Human Umbilical Vein Endothelial Cells Humans Intraocular Pressure physiology Limbus Corneae metabolism Male Ocular Hypertension metabolism Rats Rats, Sprague-Dawley Tonometry, Ocular Trabecular Meshwork drug effects Trabecular Meshwork metabolism Actins metabolism Disease Models, Animal Intraocular Pressure drug effects Limbus Corneae drug effects Ocular Hypertension drug therapy Receptors, Vasoactive Intestinal Peptide, Type II metabolism Vasoactive Intestinal Peptide pharmacology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Invest Ophthalmol Vis Sci] 2018 Jun 01; Vol. 59 (7), pp. 2848-2860. MeSH Terms: Disease Models, Animal* ; Actins / metabolism ; Intraocular Pressure / drug effects ; Limbus Corneae / drug effects ; Ocular Hypertension / drug therapy ; Receptors, Vasoactive Intestinal Peptide, Type II / metabolism ; Vasoactive Intestinal Peptide / pharmacology ; Animals ; Blotting, Western ; Chronic Disease ; Fluorescent Antibody Technique, Indirect ; Human Umbilical Vein Endothelial Cells ; Humans ; Intraocular Pressure / physiology ; Limbus Corneae / metabolism ; Male ; Ocular Hypertension / metabolism ; Rats ; Rats, Sprague-Dawley ; Tonometry, Ocular ; Trabecular Meshwork / drug effects ; Trabecular Meshwork / metabolism Substance Nomenclature: 0 (Actins) ; 0 (Receptors, Vasoactive Intestinal Peptide, Type II) ; 37221-79-7 (Vasoactive Intestinal Peptide) Entry Date(s): Date Created: 20180720 Date Completed: 20190304 Latest Revision: 20190304 Update Code: 20231215

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