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The calcium-activated chloride channel anoctamin-2 (Ano2) is thought to amplify transduction currents in olfactory receptor neurons (ORNs), a hypothesis supported by previous studies in dissociated neurons from Ano2 -/- mice. Paradoxically, despite a reduction in transduction currents in Ano2 -/- ORNs, their spike output for odor stimuli may be higher. We examined the role of Ano2 in ORNs in their native environment in freely breathing mice by imaging activity in ORN axons as they arrive in the olfactory bulb glomeruli. Odor-evoked responses in ORN axons of Ano2 -/- animals were consistently larger for a variety of odorants and concentrations. In an open arena, Ano2 -/- animals took longer to approach a localized odor source than Ano2 +/+ animals, revealing clear olfactory behavioral deficits. Our studies provide the first in vivo evidence toward an alternative or additional role for Ano2 in the olfactory transduction cascade, where it may serve as a feedback mechanism to clamp ORN spike output.

Titel:	Calcium-activated chloride channels clamp odor-evoked spike activity in olfactory receptor neurons.
Autor/in / Beteiligte Person:	Zak, JD ; Grimaud, J ; Li, RC ; Lin, CC ; Murthy, VN
Link:	Zum Volltext
Zeitschrift:	Scientific reports, Jg. 8 (2018-07-13), Heft 1, S. 10600
Veröffentlichung:	London : Nature Publishing Group, copyright 2011-, 2018
Medientyp:	academicJournal
ISSN:	2045-2322 (electronic)
DOI:	10.1038/s41598-018-28855-3
Schlagwort:	Animals Anoctamins genetics Feedback, Physiological Intravital Microscopy Mice Mice, Inbred C57BL Mice, Knockout Microscopy, Fluorescence Odorants Olfactory Bulb cytology Signal Transduction physiology Anoctamins metabolism Evoked Potentials, Somatosensory physiology Olfactory Bulb physiology Olfactory Receptor Neurons metabolism Smell physiology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, N.I.H., Extramural Language: English [Sci Rep] 2018 Jul 13; Vol. 8 (1), pp. 10600. <i>Date of Electronic Publication: </i>2018 Jul 13. MeSH Terms: Anoctamins / metabolism ; Evoked Potentials, Somatosensory / physiology ; Olfactory Bulb / physiology ; Olfactory Receptor Neurons / metabolism ; Smell / *physiology ; Animals ; Anoctamins / genetics ; Feedback, Physiological ; Intravital Microscopy ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Microscopy, Fluorescence ; Odorants ; Olfactory Bulb / cytology ; Signal Transduction / physiology References: J Neurosci. 1991 Nov;11(11):3624-9. (PMID: 1941099) ; Science. 2008 Feb 22;319(5866):1108-11. (PMID: 18292344) ; Neuron. 2004 May 27;42(4):553-66. (PMID: 15157418) ; J Neurophysiol. 2007 Jul;98(1):153-60. (PMID: 17460108) ; Proc Natl Acad Sci U S A. 2009 Jul 14;106(28):11776-81. (PMID: 19561302) ; Cell. 2016 Jun 16;165(7):1734-1748. (PMID: 27238024) ; Nat Rev Neurosci. 2014 Oct;15(10):637-54. (PMID: 25234263) ; Sci Rep. 2016 Jul 08;6:29308. (PMID: 27388949) ; Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):11078-11087. (PMID: 27647918) ; Nature. 1993 Nov 18;366(6452):283-6. (PMID: 8232590) ; Proc Natl Acad Sci U S A. 2018 May 22;115(21):5570-5575. (PMID: 29735665) ; Nat Commun. 2016 Dec 19;7:13791. (PMID: 27991499) ; Neuron. 2017 Nov 15;96(4):883-896.e7. (PMID: 29056299) ; Cell. 2005 Aug 12;122(3):473-83. (PMID: 16096065) ; Neuron. 2012 Apr 12;74(1):179-92. (PMID: 22500639) ; Nat Neurosci. 2009 Feb;12(2):210-20. (PMID: 19151709) ; Cell Rep. 2017 Oct 24;21(4):1048-1062. (PMID: 29069587) ; J Neurophysiol. 1996 Apr;75(4):1386-91. (PMID: 8727385) ; Nature. 2011 Sep 21;478(7368):241-5. (PMID: 21937988) ; PLoS One. 2015 Nov 11;10(11):e0142160. (PMID: 26558388) ; J Neurosci. 2004 Sep 8;24(36):7931-8. (PMID: 15356206) ; Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):6052-7. (PMID: 20231443) ; J Neurosci. 2015 Jan 7;35(1):146-60. (PMID: 25568110) ; Biophys J. 1997 Aug;73(2):1110-7. (PMID: 9251827) ; PLoS Biol. 2008 Apr 8;6(4):e82. (PMID: 18399719) ; Nat Neurosci. 2007 May;10(5):631-9. (PMID: 17450136) ; Physiology (Bethesda). 2012 Aug;27(4):200-12. (PMID: 22875451) ; Nat Neurosci. 2014 Sep;17(9):1225-32. (PMID: 25086608) ; Am J Physiol Cell Physiol. 2001 Jun;280(6):C1387-93. (PMID: 11350733) ; J Neurosci. 1998 Sep 1;18(17):6623-30. (PMID: 9712634) ; J Neurosci. 2005 Apr 6;25(14):3586-92. (PMID: 15814789) ; Front Cell Neurosci. 2009 Aug 27;3:9. (PMID: 19753143) ; Physiol Rep. 2017 Aug;5(15):null. (PMID: 28784854) ; Pflugers Arch. 2009 Oct;458(6):1023-38. (PMID: 19475416) ; J Physiol. 2010 Jul 1;588(Pt 13):2305-14. (PMID: 20421283) ; Proc Natl Acad Sci U S A. 2013 May 14;110(20):8248-53. (PMID: 23630284) ; Nat Rev Neurosci. 2009 Aug;10(8):611-8. (PMID: 19584894) ; Neuron. 2011 Sep 22;71(6):962-73. (PMID: 21943596) ; Curr Opin Neurobiol. 2015 Oct;34:1-7. (PMID: 25616211) ; Physiol Rev. 1998 Apr;78(2):429-66. (PMID: 9562035) ; Chem Senses. 2015 Feb;40(2):73-87. (PMID: 25500808) ; J Physiol. 2011 May 1;589(Pt 9):2261-73. (PMID: 21486768) ; Nature. 2014 Nov 13;515(7526):269-73. (PMID: 25383519) ; J Neurophysiol. 1995 Feb;73(2):562-7. (PMID: 7760118) ; J Physiol. 2009 May 15;587(Pt 10):2127-39. (PMID: 19015192) ; Nat Neurosci. 2012 Mar 11;15(4):628-35. (PMID: 22406548) ; Am J Respir Crit Care Med. 2013 Feb 15;187(4):374-81. (PMID: 23239156) ; Neuron. 2013 Nov 20;80(4):1010-24. (PMID: 24139818) ; J Gen Physiol. 2016 Oct;148(4):293-311. (PMID: 27619419) ; Nat Neurosci. 2011 Jun;14(6):763-9. (PMID: 21516098) ; Am J Obstet Gynecol. 2014 Dec;211(6):688.e1-10. (PMID: 24928056) ; J Physiol. 2003 Jan 15;546(Pt 2):363-74. (PMID: 12527724) Grant Information: F32 DC015938 United States DC NIDCD NIH HHS; R01 DC014453 United States DC NIDCD NIH HHS; R01 DC014941 United States DC NIDCD NIH HHS; R01 DC14454 International U.S. Department of Health & Human Services \| NIH \| National Institute on Deafness and Other Communication Disorders (NIDCD) Substance Nomenclature: 0 (ANO2 protein, mouse) ; 0 (Anoctamins) Entry Date(s): Date Created: 20180715 Date Completed: 20191028 Latest Revision: 20200629 Update Code: 20231215 PubMed Central ID: PMC6045664

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Calcium-activated chloride channels clamp odor-evoked spike activity in olfactory receptor neurons.