Effect of Icariin on apoptosis and expression of Fas, Fas ligand, B cell lymphoma, and Bcl-2-associated X protein in CD4+ T lymphocytes from patients with ankylosing spondylitis.

Wang, H ; Jiang, Q ; et al.

In: Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, Jg. 37 (2017-04-01), Heft 2, S. 207

academicJournal

Objective: To investigate the effects of icariin on apoptosis and the expression of Fas, Fas ligand (FasL), B cell lymphoma (Bcl-2), and Bcl-2-associated X protein (Bax) in CD4＋ T lymphocytes from patients with ankylosing spondylitis.

Methods: Primary cultures of peripheral blood CD4+ T lymphocytes were established and treated with icariin at high, medium, and low doses (0.5, 0.25, and 0.125 mg/mL). Sulfasalazine treated and helthy cells were used as controls. Apoptosis of treated cells was determined by flow cytometry. Reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assays were used to determine the effects of icariin on the expression of Fas, FasL, Bcl-2, and Bax. The activity of caspase 8 and caspase 3 was determined by a colorimetric assay.

Results: The mRNA and protein expression of Fas, and activity of caspase 8 and caspase 3 in CD4+ T lymphocytes were increased by icariin (P < 0.05). Conversely, the mRNA and protein expression of Bcl-2 was decreased (P < 0.05). The expression of FasL and Bax were not significantly different between groups. The proapoptotic effects of icariin were dose-dependent.

Conclusion: Icariin induces the apoptosis of CD4 + T cells from patients with AS comparing to normal control. Therefore, the induction of apoptosis may be the likely mechanism of action of icariin's antirheumatics activities.

Titel:	Effect of Icariin on apoptosis and expression of Fas, Fas ligand, B cell lymphoma, and Bcl-2-associated X protein in CD4+ T lymphocytes from patients with ankylosing spondylitis.
Autor/in / Beteiligte Person:	Wang, H ; Jiang, Q ; Feng, X
Zeitschrift:	Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, Jg. 37 (2017-04-01), Heft 2, S. 207
Veröffentlichung:	Beijing : Academy Of Traditional Chinese Medicine ; <i>Original Publication</i>: [Beijing, China : Pub. Office, Journal of Traditional Chinese Medicine, 1981-, 2017
Medientyp:	academicJournal
ISSN:	0255-2922 (print)
DOI:	10.1016/s0254-6272(17)30046-8
Schlagwort:	Adult CD4-Positive T-Lymphocytes drug effects CD4-Positive T-Lymphocytes metabolism Fas Ligand Protein metabolism Female Humans Male Middle Aged Proto-Oncogene Proteins c-bcl-2 metabolism Spondylitis, Ankylosing genetics Spondylitis, Ankylosing metabolism Young Adult bcl-2-Associated X Protein metabolism fas Receptor metabolism Apoptosis drug effects Drugs, Chinese Herbal administration & dosage Fas Ligand Protein genetics Flavonoids administration & dosage Proto-Oncogene Proteins c-bcl-2 genetics Spondylitis, Ankylosing drug therapy bcl-2-Associated X Protein genetics fas Receptor genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't Language: English [J Tradit Chin Med] 2017 Apr; Vol. 37 (2), pp. 207-13. MeSH Terms: Apoptosis / drug effects ; Drugs, Chinese Herbal / administration & dosage ; Fas Ligand Protein / genetics ; Flavonoids / administration & dosage ; Proto-Oncogene Proteins c-bcl-2 / genetics ; Spondylitis, Ankylosing / drug therapy ; bcl-2-Associated X Protein / genetics ; fas Receptor / genetics ; Adult ; CD4-Positive T-Lymphocytes / drug effects ; CD4-Positive T-Lymphocytes / metabolism ; Fas Ligand Protein / metabolism ; Female ; Humans ; Male ; Middle Aged ; Proto-Oncogene Proteins c-bcl-2 / metabolism ; Spondylitis, Ankylosing / genetics ; Spondylitis, Ankylosing / metabolism ; Young Adult ; bcl-2-Associated X Protein / metabolism ; fas Receptor / metabolism Substance Nomenclature: 0 (Drugs, Chinese Herbal) ; 0 (FAS protein, human) ; 0 (FASLG protein, human) ; 0 (Fas Ligand Protein) ; 0 (Flavonoids) ; 0 (Proto-Oncogene Proteins c-bcl-2) ; 0 (bcl-2-Associated X Protein) ; 0 (fas Receptor) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20180702 Date Completed: 20180731 Latest Revision: 20191027 Update Code: 20240513

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