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Background/aims: Glucocorticoid (GC)-related osteonecrosis of the femoral head (ONFH) is a common complication following administration of steroids to treat many diseases. Our previous study demonstrated that icariin (ICA) might have a beneficial effect on the bone marrow mesenchymal stem cells (BMSCs) of patients with steroid-associated osteonecrosis. In this study, we investigated the underlying mechanisms of ICA associated with the potential enhancement of osteogenesis and anti-adipogenesis in GC-related ONFH.

Methods: In vitro cell proliferation was evaluated by CCK-8 assay. Alizarin red S and alkaline phosphatase (ALP) activity were used to measure osteogenic differentiation, while adipogenic differentiation was revealed by oil red O staining and TG content assay. The expression level of osteogenesis-associated genes and PPARγ was evaluated by RT-qPCR, western blotting and immunofluorescence. A total of 30 female SD rats were randomly separated into three groups: a control group, a methylprednisolone (MPS) group and a MPS + ICA group. Serum ALP and TG (triglyceride), micro-CT scanning, histological and immunohistochemical analyses were performed in the animal model.

Results: In the in vitro study, ICA promoted proliferation, improved osteogenic differentiation and suppressed adipogenic differentiation of BMSCs treated with MPS. The group treated with MPS and 10-6 M ICA expressed higher levels of Runx2, ALP, bone morphogenetic protein (BMP) 2, and OC and lower expression of PPARγ than the MPS group. In the in vivo study, ICA prevented bone loss in a rat model of GC-related ONFH as shown by micro-CT scanning, histological and immunohistochemical analyses.

Conclusions: ICA is an effective compound for promoting bone repair and preventing or delaying the progression of GC-associated ONFH in rats. This effect can be explained by its ability to improve the balance between adipogenesis and osteogenesis, indicating that ICA is an effective candidate for management of GC-associated ONFH.

Titel:	Icariin Protects against Glucocorticoid-Induced Osteonecrosis of the Femoral Head in Rats.
Autor/in / Beteiligte Person:	Huang, Z ; Cheng, C ; Cao, B ; Wang, J ; Wei, H ; Liu, X ; Han, Y ; Yang, S ; Wang, X
Link:	Zum Volltext
Zeitschrift:	Cellular physiology and biochemistry : international journal of experimental cellular, Jg. 47 (2018), Heft 2, S. 694
Veröffentlichung:	2019- : Düsseldorf, Germany : Cell Physiol Biochem Press GmbH & Co KG ; <i>Original Publication</i>: Basel ; New York : S. Karger, 1991-2018., 2018
Medientyp:	academicJournal
ISSN:	1421-9778 (electronic)
DOI:	10.1159/000490023
Schlagwort:	Adipogenesis drug effects Alkaline Phosphatase metabolism Animals Bone Marrow Cells cytology Bone Morphogenetic Protein 2 metabolism Cell Differentiation drug effects Cell Proliferation drug effects Cells, Cultured Core Binding Factor Alpha 1 Subunit metabolism Female Femur Head Necrosis diagnostic imaging Femur Head Necrosis pathology Femur Head Necrosis veterinary Flavonoids therapeutic use Glucocorticoids pharmacology Mesenchymal Stem Cells cytology Mesenchymal Stem Cells drug effects Mesenchymal Stem Cells metabolism Methylprednisolone pharmacology PPAR gamma metabolism Rats Rats, Sprague-Dawley Femur Head Necrosis prevention & control Flavonoids pharmacology Osteogenesis drug effects Protective Agents pharmacology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Cell Physiol Biochem] 2018; Vol. 47 (2), pp. 694-706. <i>Date of Electronic Publication: </i>2018 May 22. MeSH Terms: Femur Head Necrosis / prevention & control ; Flavonoids / pharmacology ; Osteogenesis / drug effects ; Protective Agents / pharmacology ; Adipogenesis / drug effects ; Alkaline Phosphatase / metabolism ; Animals ; Bone Marrow Cells / cytology ; Bone Morphogenetic Protein 2 / metabolism ; Cell Differentiation / drug effects ; Cell Proliferation / drug effects ; Cells, Cultured ; Core Binding Factor Alpha 1 Subunit / metabolism ; Female ; Femur Head Necrosis / diagnostic imaging ; Femur Head Necrosis / pathology ; Femur Head Necrosis / veterinary ; Flavonoids / therapeutic use ; Glucocorticoids / pharmacology ; Mesenchymal Stem Cells / cytology ; Mesenchymal Stem Cells / drug effects ; Mesenchymal Stem Cells / metabolism ; Methylprednisolone / pharmacology ; PPAR gamma / metabolism ; Rats ; Rats, Sprague-Dawley Contributed Indexing: Keywords: Glucocorticoid (GC); Icariin (ICA); Osteonecrosis of the femoral head (ONFH); PPARγ; Runx2 Substance Nomenclature: 0 (Bmp2 protein, rat) ; 0 (Bone Morphogenetic Protein 2) ; 0 (Core Binding Factor Alpha 1 Subunit) ; 0 (Flavonoids) ; 0 (Glucocorticoids) ; 0 (PPAR gamma) ; 0 (Protective Agents) ; 0 (Runx2 protein, rat) ; EC 3.1.3.1 (Alkaline Phosphatase) ; VNM47R2QSQ (icariin) ; X4W7ZR7023 (Methylprednisolone) Entry Date(s): Date Created: 20180526 Date Completed: 20180724 Latest Revision: 20190212 Update Code: 20231215

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Icariin Protects against Glucocorticoid-Induced Osteonecrosis of the Femoral Head in Rats.