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Aims: Cell communication between mesenchymal stem cells and blood vessel cells are crucial for bone repair. We have previously shown that the phyto-molecule icariin significantly promoted osteogenic differentiation of rat adipose-derived stem cells (ASCs). In the present study, we aimed to investigate the relationship between icariin induced osteogenic differentiation of ASCs and angiogenesis of rat endothelial progenitor cells (EPCs). Besides, we used icariin doped 45S5 Bioglass seeded with ASCs to promote bone healing in rat calvarial bone defect models.

Main Methods: The conditioned medium from undifferentiated ASCs (ASCs-CM) and icariin induced ASCs (Icariin-ASCs-CM) was obtained and the vascular endothelial growth factor (VEGF) protein secretion level was measured. The angiogenic capacity and molecular mechanism of ASC-CM and Icariin-ASCs-CM on rat EPCs was analyzed. Rat calvarial bone defect models were established and treated with scaffolds implantation. Micro-CT imaging, histological and immunohistological staining were performed on the isolated specimens at 12 weeks post-surgery.

Key Findings: VEGF protein expression was significantly increased after icariin treatment with the highest expression in the 10 -7  M icariin group. Icariin-ASCs-CM obviously increased the angiogenesis of rat EPCs and this capacity was inhibited by a VEGF/VEGF receptor-specific binding inhibitor bevacizumab. Results of the in vivo investigations showed that all scaffolds promoted bone healing compared to the Control group. Icariin significantly improved the healing capacity of 45S5 Bioglass seeded with ASCs.

Significance: Implantation of Icariin/45S5 Bioglass seeded with rat ASCs could obviously promote both osteogenesis and angiogenesis and therefore represents an ideal candidate bone substitutes for bone repair and regeneration.

Titel:	Icariin doped bioactive glasses seeded with rat adipose-derived stem cells to promote bone repair via enhanced osteogenic and angiogenic activities.
Autor/in / Beteiligte Person:	Jing, X ; Yin, W ; Tian, H ; Chen, M ; Yao, X ; Zhu, W ; Guo, F ; Ye, Y
Link:	Zum Volltext
Zeitschrift:	Life sciences, Jg. 202 (2018-06-01), S. 52
Veröffentlichung:	<2008->: Amsterdam : Elsevier ; <i>Original Publication</i>: Oxford; Elmsford, N. Y. [etc.] Pergamon Press., 2018
Medientyp:	academicJournal
ISSN:	1879-0631 (electronic)
DOI:	10.1016/j.lfs.2018.02.026
Schlagwort:	Animals Ceramics Culture Media, Conditioned Endothelial Cells transplantation Glass Immunohistochemistry Male Rats Rats, Sprague-Dawley Skull pathology Tissue Scaffolds Vascular Endothelial Growth Factor A biosynthesis Adipose Tissue cytology Bone Regeneration drug effects Flavonoids pharmacology Neovascularization, Physiologic drug effects Osteogenesis drug effects Stem Cells drug effects
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Life Sci] 2018 Jun 01; Vol. 202, pp. 52-60. <i>Date of Electronic Publication: </i>2018 Feb 20. MeSH Terms: Adipose Tissue / cytology ; Bone Regeneration / drug effects ; Flavonoids / pharmacology ; Neovascularization, Physiologic / drug effects ; Osteogenesis / drug effects ; Stem Cells / drug effects ; Animals ; Ceramics ; Culture Media, Conditioned ; Endothelial Cells / transplantation ; Glass ; Immunohistochemistry ; Male ; Rats ; Rats, Sprague-Dawley ; Skull / pathology ; Tissue Scaffolds ; Vascular Endothelial Growth Factor A / biosynthesis Contributed Indexing: Keywords: 45S5 Bioglass; Adipose-derived stem cells; Angiogenesis; Bone repair and regeneration; Icariin; Vascular endothelial growth factor Substance Nomenclature: 0 (Culture Media, Conditioned) ; 0 (Flavonoids) ; 0 (Vascular Endothelial Growth Factor A) ; 0 (bioactive glass 45S5) ; 0 (vascular endothelial growth factor A, rat) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20180223 Date Completed: 20180521 Latest Revision: 20180521 Update Code: 20240513

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Icariin doped bioactive glasses seeded with rat adipose-derived stem cells to promote bone repair via enhanced osteogenic and angiogenic activities.