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Background: Icariin (ICA), a major ingredient of Epimediumbrevicornum, has various pharmacological activities including central nervous system protective functions such as the improvement of learning and memory function in mice models of Alzheimer's disease. It has been reported that ICA can promote regeneration of peripheral nerve and functional recovery. The purpose of this study was to investigate the potentiating effect of ICA on the proliferation of rat hippocampal neural stem cells, and explore the possible mechanism involved.

Methods: Primary neural stem cells were prepared from the hippocampus of newly born SD rats, and cells were cultured in special stem cell culture medium. Neural stem cells were confirmed by immunofluorescence detection of nestin, NSE and GFAP expression. The effect of ICA on the growth and proliferation of the neural stem cells was evaluated by 5-ethynyl-2-deoxyuridine (EdU) labeling of proliferating cells, and photomicrographic images of the cultured neural stem cells. Further, the mechanism of ICA-induced cell proliferation of neural stem cells was investigated by analyzing the gene and protein expression of cell cycle related genes cyclin D1 and p21.

Results: The present study showed that icariin promotes the growth and proliferation of neural stem cells from rat hippocampus in a dose-dependent manner. Incubation of cells with icariin resulted in significant increase in the number of stem cell spheres as well as the increased incorporation of EdU when compared with cells exposed to control vehicle. In addition, it was found that icariin-induced effect on neural stem cells is associated with increased mRNA and protein expression of cell cycle genes cyclin D1 and p21.

Conclusions: This study evidently demonstrates the potentiating effect of ICA on neural stem cell growth and proliferation, which might be mediated through regulation of cell cycle gene and protein expression promoting cell cycle progression.

Titel:	Stimulatory effect of icariin on the proliferation of neural stem cells from rat hippocampus.
Autor/in / Beteiligte Person:	Fu, X ; Li, S ; Zhou, S ; Wu, Q ; Jin, F ; Shi, J
Link:	Zum Volltext
Zeitschrift:	BMC complementary and alternative medicine, Jg. 18 (2018-01-29), Heft 1, S. 34
Veröffentlichung:	London : BioMed Central, [2001-2019], 2018
Medientyp:	academicJournal
ISSN:	1472-6882 (electronic)
DOI:	10.1186/s12906-018-2095-y
Schlagwort:	Animals Cells, Cultured Cyclin D1 metabolism Cyclin-Dependent Kinase Inhibitor p21 metabolism Neural Stem Cells cytology Neural Stem Cells metabolism Rats Rats, Sprague-Dawley Cell Proliferation drug effects Flavonoids pharmacology Hippocampus cytology Neural Stem Cells drug effects
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [BMC Complement Altern Med] 2018 Jan 29; Vol. 18 (1), pp. 34. <i>Date of Electronic Publication: </i>2018 Jan 29. MeSH Terms: Cell Proliferation / drug effects ; Flavonoids / pharmacology ; Hippocampus / cytology ; Neural Stem Cells / drug effects ; Animals ; Cells, Cultured ; Cyclin D1 / metabolism ; Cyclin-Dependent Kinase Inhibitor p21 / metabolism ; Neural Stem Cells / cytology ; Neural Stem Cells / metabolism ; Rats ; Rats, Sprague-Dawley References: Eur J Pharmacol. 2010 Jan 25;626(2-3):213-8. (PMID: 19782061) ; Mol Neurobiol. 2016 May;53(4):2124-31. (PMID: 25934102) ; Mol Nutr Food Res. 2011 Apr;55(4):613-22. (PMID: 21462329) ; Molecules. 2011 Dec 06;16(12 ):10123-33. (PMID: 22146373) ; J Ethnopharmacol. 2007 Dec 3;114(3):412-6. (PMID: 17890032) ; Mol Cancer. 2015 Mar 11;14 :57. (PMID: 25889927) ; Mod Pathol. 2014 Jan;27(1):87-95. (PMID: 23887292) ; PLoS One. 2015 Mar 25;10 (3):e0119955. (PMID: 25806530) ; Cancer Treat Res. 2004;119:217-37. (PMID: 15164880) ; J Neurosci. 2000 Mar 15;20(6):2218-28. (PMID: 10704497) ; PLoS One. 2013 Oct 17;8(10):e76860. (PMID: 24146937) ; Neural Regen Res. 2015 Jan;10(1):84-9. (PMID: 25788925) ; Phytomedicine. 2010 Oct;17 (12 ):950-5. (PMID: 20382007) ; Lancet Neurol. 2003 Sep;2(9):539-47. (PMID: 12941576) ; Lancet. 2016 Oct 8;388(10053):1545-1602. (PMID: 27733282) ; Cell Stem Cell. 2013 Jan 3;12(1):88-100. (PMID: 23260487) ; Neurol India. 2011 Jul-Aug;59(4):558-65. (PMID: 21891934) ; Neuropharmacology. 2010 Jul-Aug;59(1-2):70-6. (PMID: 20381504) ; Biochem Biophys Res Commun. 2016 Sep 16;478(2):956-63. (PMID: 27521891) ; Nat Rev Genet. 2008 Feb;9(2):115-28. (PMID: 18202695) ; Genes Dev. 2005 Mar 15;19(6):756-67. (PMID: 15769947) ; J Biol Chem. 2002 Jan 18;277(3):2207-15. (PMID: 11714699) ; Neuroscience. 2009 Dec 29;164(4):1521-30. (PMID: 19782730) ; J Nutr. 2004 Dec;134(12 ):3370-7. (PMID: 15570039) ; Urol Oncol. 2010 Sep-Oct;28(5):526-33. (PMID: 19450997) ; Life Sci. 2014 Oct 2;114(2):57-61. (PMID: 25066930) ; Life Sci. 2014 Jul 17;108(2):63-70. (PMID: 24857982) ; Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2415-20. (PMID: 18272492) ; Chin Med. 2014 Apr 09;9(1):13. (PMID: 24716802) ; Curr Protoc Stem Cell Biol. 2013;Chapter 2:Unit 2D.14. (PMID: 23404673) ; Pharmacol Biochem Behav. 2010 Sep;96(3):301-5. (PMID: 20566405) ; PLoS One. 2010 Feb 26;5(2):e9414. (PMID: 20195471) ; Mol Biol Cell. 2006 Jan;17 (1):402-12. (PMID: 16280359) ; Nat Med. 1998 Nov;4(11):1313-7. (PMID: 9809557) ; Mediators Inflamm. 2013;2013:651207. (PMID: 24311896) ; J Neurosci Res. 1995 Oct 1;42(2):184-91. (PMID: 8568918) ; Eur J Neurosci. 2003 May;17(10):2042-6. (PMID: 12786970) ; Mol Med Rep. 2013 Nov;8(5):1499-504. (PMID: 24065204) Grant Information: 81160400 National Natural Science Foundation of China; 2011CB512009 national "973" special subject Contributed Indexing: Keywords: Cyclin D1; Icariin; Neural stem cells; Proliferation; p21 Substance Nomenclature: 0 (Cyclin-Dependent Kinase Inhibitor p21) ; 0 (Flavonoids) ; 136601-57-5 (Cyclin D1) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20180131 Date Completed: 20180305 Latest Revision: 20181113 Update Code: 20240513 PubMed Central ID: PMC5789743

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Stimulatory effect of icariin on the proliferation of neural stem cells from rat hippocampus.