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Signal transduction in sensory neurons of the mammalian vomeronasal organ (VNO) involves the opening of the canonical transient receptor potential channel Trpc2, a Ca 2+ -permeable cation channel that is activated by diacylglycerol and inhibited by Ca 2+ -calmodulin. There has been a long-standing debate about the extent to which the second messenger inositol 1,4,5-trisphosphate (InsP 3 ) and type 3 InsP 3 receptor (InsP 3 R3) are involved in the opening of Trpc2 channels and in sensory activation of the VNO. To address this question, we investigated VNO function of mice carrying a knockout mutation in the Itpr3 locus causing a loss of InsP 3 R3. We established a new method to monitor Ca 2+ in the endoplasmic reticulum of vomeronasal sensory neurons (VSNs) by employing the GFP-aequorin protein sensor erGAP2. We also performed simultaneous InsP 3 photorelease and Ca 2+ monitoring experiments, and analysed Ca 2+ dynamics, sensory currents, and action potential or field potential responses in InsP 3 R3-deficient VSNs. Disruption of Itpr3 abolished or minimized the Ca 2+ transients evoked by photoactivated InsP 3 , but there was virtually no effect on sensory activation of VSNs. Therefore, InsP 3 R3 is dispensable for primary chemoelectrical transduction in mouse VNO. We conclude that InsP 3 R3 is not required for gating of Trpc2 in VSNs.

Titel:	Type 3 inositol 1,4,5-trisphosphate receptor is dispensable for sensory activation of the mammalian vomeronasal organ.
Autor/in / Beteiligte Person:	Chamero, P ; Weiss, J ; Alonso, MT ; Rodríguez-Prados, M ; Hisatsune, C ; Mikoshiba, K ; Leinders-Zufall, T ; Zufall, F
Link:	Zum Volltext
Zeitschrift:	Scientific reports, Jg. 7 (2017-08-31), Heft 1, S. 10260
Veröffentlichung:	London : Nature Publishing Group, copyright 2011-, 2017
Medientyp:	academicJournal
ISSN:	2045-2322 (electronic)
DOI:	10.1038/s41598-017-09638-8
Schlagwort:	Action Potentials Animals Calcium metabolism Calcium Signaling Calmodulin metabolism Evoked Potentials Extracellular Space metabolism Gene Expression Intracellular Space metabolism Ligands Mice Molecular Imaging Photolysis Sensory Receptor Cells physiology Signal Transduction Inositol 1,4,5-Trisphosphate Receptors genetics Inositol 1,4,5-Trisphosphate Receptors metabolism Mammals Sensation Vomeronasal Organ physiology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Sci Rep] 2017 Aug 31; Vol. 7 (1), pp. 10260. <i>Date of Electronic Publication: </i>2017 Aug 31. MeSH Terms: Mammals* ; Sensation* ; Inositol 1,4,5-Trisphosphate Receptors / genetics ; Inositol 1,4,5-Trisphosphate Receptors / metabolism ; Vomeronasal Organ / *physiology ; Action Potentials ; Animals ; Calcium / metabolism ; Calcium Signaling ; Calmodulin / metabolism ; Evoked Potentials ; Extracellular Space / metabolism ; Gene Expression ; Intracellular Space / metabolism ; Ligands ; Mice ; Molecular Imaging ; Photolysis ; Sensory Receptor Cells / physiology ; Signal Transduction References: J Neurophysiol. 2007 Oct;98(4):2357-69. (PMID: 17715188) ; Brain Res. 1999 Mar 27;823(1-2):161-8. (PMID: 10095022) ; J Exp Biol. 2006 May;209(Pt 10):1914-27. (PMID: 16651557) ; Handb Exp Pharmacol. 2014;223:v - vi. 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Type 3 inositol 1,4,5-trisphosphate receptor is dispensable for sensory activation of the mammalian vomeronasal organ.