Dissemination of Carbapenem-resistant Klebsiella pneumoniae clinical isolates with various combinations of Carbapenemases (KPC-2, NDM-1, NDM-4, and OXA-48) and 16S rRNA Methylases (RmtB and RmtC) in Vietnam.
In: BMC infectious diseases, Jg. 17 (2017-07-04), Heft 1, S. 467
Online
academicJournal
Zugriff:
Methods: Twenty-seven clinical isolates of carbapenem-resistant Klebsiella pneumoniae with MICs ≥4 mg/L for imipenem or meropenem were obtained from inpatients in a hospital in Vietnam. Antimicrobial susceptibility tests and whole genome sequencing were performed. Multilocus sequence typing and the presence of drug resistant genes were determined and a maximum-likelihood phylogenetic tree was constructed by SNP alignment of whole genome sequencing data.
Results: All the isolates harbored one of genes encoding carbapenemases, including KPC-2, NDM-1, NDM-4 and OXA-48. Of the isolates, 13 were resistant to arbekacin with MICs ≥256 mg/L and to amikacin with MICs ≥512 mg/L. These isolates harbored a gene encoding a 16S rRNA methylase, either RmtB or RmtC. Eighteen and 4 isolates belonged to international clones, ST15 and ST16, respectively. None of the isolates had colistin-resistant factors.
Conclusion: Carbapenem-resistant K. pneumoniae isolates belonged to international clones spread in a medical setting in Vietnam, and that these isolates harbored genes encoding various combinations of carbapenemases and 16S rRNA methylases. This is the first report of KPC-2, NDM-4 and OXA-48 producers in a medical setting in Vietnam.
Titel: |
Dissemination of Carbapenem-resistant Klebsiella pneumoniae clinical isolates with various combinations of Carbapenemases (KPC-2, NDM-1, NDM-4, and OXA-48) and 16S rRNA Methylases (RmtB and RmtC) in Vietnam.
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Autor/in / Beteiligte Person: | Tada, T ; Tsuchiya, M ; Shimada, K ; Nga, TTT ; Thu, LTA ; Phu, TT ; Ohmagari, N ; Kirikae, T |
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Zeitschrift: | BMC infectious diseases, Jg. 17 (2017-07-04), Heft 1, S. 467 |
Veröffentlichung: | London : BioMed Central, [2001-, 2017 |
Medientyp: | academicJournal |
ISSN: | 1471-2334 (electronic) |
DOI: | 10.1186/s12879-017-2570-y |
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