Analysis of blaSHV-12-carrying Escherichia coli clones and plasmids from human, animal and food sources.
In: The Journal of antimicrobial chemotherapy, Jg. 72 (2017-06-01), Heft 6, S. 1589-1596
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Objectives: This study aimed at characterizing 23 Escherichia coli isolates from various sources and their respective bla SHV-12 -carrying plasmids and sequencing one of these plasmids completely.
Methods: Isolates were typed by XbaI-PFGE, MLST and PCR-based phylotyping. Transformed bla SHV-12 -carrying plasmids were examined by replicon typing, S1-nuclease, conjugation, EcoRI-HindIII-BamHI digests and plasmid MLST. Co-located resistance genes and integrons as well as the bla SHV-12 genetic environment were analysed by PCR and sequencing. One IncI1 plasmid was sequenced completely using HiSeq 2500 and gap closure by PCRs and Sanger sequencing.
Results: Among the 23 SHV-12-positive E. coli , some isolates from different sources showed the same characteristics: ST23/phylogroup A (human, dog, livestock), ST57/D (wild bird, chicken meat) and ST117/D (chicken meat, chicken). All bla SHV-12 genes were horizontally transferable via 30-120 kb plasmids of incompatibility groups IncI1 ( n = 17), IncK ( n = 3), IncF ( n = 1), IncX3 ( n = 1) and a non-typeable plasmid. IncK plasmids, indistinguishable in size and restriction patterns, were found in isolates from different sources (ST57/D, meat; ST131/B2, meat; ST57/B1, dog). The IncI1- bla SHV-12 -carrying plasmids were mostly assigned to plasmid ST (pST) 26 and pST3. Three plasmids showed novel pSTs (pST214, pST215). The majority of the IncI1 transformants exhibited resistance to β-lactams, chloramphenicol and streptomycin (in relation with a class 1 integron containing an estX - psp - aadA2 - cmlA1 - aadA1 - qacI gene cassette array), and to tetracycline. A novel bla SHV-12 environment was detected and whole plasmid sequencing revealed a Tn 21 -derived- bla SHV12 -ΔTn 1721 resistance complex.
Conclusions: Results from this study suggest that the dissemination of bla SHV-12 genes occurs by vertical (clonal) and horizontal transfer, the latter mainly mediated through IncI1 multidrug-resistance plasmids.
(© The Author 2017. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)
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Analysis of blaSHV-12-carrying Escherichia coli clones and plasmids from human, animal and food sources.
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Autor/in / Beteiligte Person: | Alonso, CA ; Michael, GB ; Li, J ; Somalo, S ; Simón, C ; Wang, Y ; Kaspar, H ; Kadlec, K ; Torres, C ; Schwarz, S |
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Zeitschrift: | The Journal of antimicrobial chemotherapy, Jg. 72 (2017-06-01), Heft 6, S. 1589-1596 |
Veröffentlichung: | 1997- : London : Oxford University Press ; <i>Original Publication</i>: London, New York, Academic Press., 2017 |
Medientyp: | academicJournal |
ISSN: | 1460-2091 (electronic) |
DOI: | 10.1093/jac/dkx024 |
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