Einzeltreffer — DigiBib

Context: The effects of icariin, a chief constituent of ﬂavonoids from Epimedium brevicornum Maxim (Berberidaceae), on the levels of HIF-1α, HSP-60 and HSP-70 remain unknown.

Objective: To explore the effects of icariin on the levels of HSP-60, HIF-1α and HSP-70 neuron-specific enolase (NSE) and cell viability.

Materials and Methods: PC12 cells were treated with icariin (10 -7 , 10 -6 or 10 -5 mol/L) for 3 h (1 h before oxygen-glucose deprivation (OGD) plus 2 h OGD). HSP-60, HIF-1α, HSP-70 and NSE were measured using enzyme-linked immunosorbent assay (ELISA). Cell viability was determined by metabolic 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.

Results: After 2 h OGD, levels of HIF-1α, HSP-60, HSP-70 and NSE were increased significantly (HIF-1α: 33.3 ± 1.9 ng/L, HSP-60: 199 ± 16 ng/L, HSP-70: 195 ± 17 ng/L, NSE: 1487 ± 125 ng/L), and cell viability was significantly decreased (0.26 ± 0.03), while icariin (10 -7 , 10 -6 , or 10 -5 mol/L) significantly reduced the contents of HIF-1α, HSP-60, HSP-70 and NSE (HIF-1α: 14.1 ± 1.4, 22.6 ± 1.8, 15.7 ± 2.1, HSP-60: 100 ± 12, 89 ± 6, 113 ± 11, HSP-70: 139 ± 9, 118 ± 7, 95 ± 9 and NSE: 1121 ± 80, 1019 ± 52, 731 ± 88), and improved cell viability (0.36 ± 0.03, 0.38 ± 0.04, 0.37 ± 0.03) in OGD-treated PC12 cells.

Discussion and Conclusion: These results indicate that the protective mechanisms of icariin against OGD-induced injury may be related to down-regulating the expression of HIF-1α, HSP-60 and HSP-70.

Titel:	The effects of icariin on the expression of HIF-1α, HSP-60 and HSP-70 in PC12 cells suffered from oxygen-glucose deprivation-induced injury.
Autor/in / Beteiligte Person:	Mo, ZT ; Li, WN ; Zhai, YR ; Gao, SY
Link:	Zum Volltext
Zeitschrift:	Pharmaceutical biology, Jg. 55 (2017-12-01), Heft 1, S. 848
Veröffentlichung:	[London] : Taylor & Francis ; <i>Original Publication</i>: Lisse, the Netherlands : Swets & Zeitlinger, c1998-, 2017
Medientyp:	academicJournal
ISSN:	1744-5116 (electronic)
DOI:	10.1080/13880209.2017.1281968
Schlagwort:	Animals Cell Hypoxia Cell Survival drug effects Down-Regulation Enzyme-Linked Immunosorbent Assay PC12 Cells Phosphopyruvate Hydratase analysis Rats Chaperonin 60 analysis Flavonoids pharmacology HSP70 Heat-Shock Proteins analysis Hypoxia-Inducible Factor 1, alpha Subunit analysis Neuroprotective Agents pharmacology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Pharm Biol] 2017 Dec; Vol. 55 (1), pp. 848-852. MeSH Terms: Chaperonin 60 / analysis ; Flavonoids / pharmacology ; HSP70 Heat-Shock Proteins / analysis ; Hypoxia-Inducible Factor 1, alpha Subunit / analysis ; Neuroprotective Agents / *pharmacology ; Animals ; Cell Hypoxia ; Cell Survival / drug effects ; Down-Regulation ; Enzyme-Linked Immunosorbent Assay ; PC12 Cells ; Phosphopyruvate Hydratase / analysis ; Rats References: J Mol Neurosci. 2013 Sep;51(1):180-6. (PMID: 23420039) ; Sci Rep. 2015 Aug 07;5:12958. (PMID: 26248657) ; J Biol Chem. 2002 Apr 26;277(17):15028-34. (PMID: 11836257) ; Cancer Res. 2005 Feb 1;65(3):999-1006. (PMID: 15705901) ; Chin J Traumatol. 2012;15(6):338-41. (PMID: 23186922) ; Glia. 2015 Jul;63(7):1200-12. (PMID: 25802219) ; J Neuroinflammation. 2014 Apr 23;11:81. (PMID: 24755298) ; Acta Pharmacol Sin. 2012 Jun;33(6):737-42. (PMID: 22543703) ; J Am Chem Soc. 2010 Sep 1;132(34):11870-1. (PMID: 20695501) ; J Biol Chem. 2007 Oct 26;282(43):31289-301. (PMID: 17823127) ; Brain Res. 2010 Jun 2;1334:73-83. (PMID: 20380820) ; Life Sci. 2015 Apr 1;126:57-68. (PMID: 25634110) ; Pflugers Arch. 2006 Jan;451(4):534-43. (PMID: 16208522) ; Am J Physiol Gastrointest Liver Physiol. 2010 Oct;299(4):G833-43. (PMID: 20689059) ; Surg Neurol. 1995 Mar;43(3):267-70; discussion 270-1. (PMID: 7540773) ; Chin Med J (Engl). 2005 Oct 5;118(19):1637-43. (PMID: 16232349) ; Exp Neurol. 2005 Sep;195(1):257-66. (PMID: 15936758) ; Clin Chim Acta. 1983 Jul 15;131(3):165-70. (PMID: 6883712) ; J Orthop Res. 2014 Aug;32(8):975-80. (PMID: 24677016) ; Neuropharmacology. 2010 Jul-Aug;59(1-2):70-6. (PMID: 20381504) ; Circ J. 2011;75(10):2445-52. (PMID: 21817814) ; Drug Des Devel Ther. 2014 May 28;8:639-50. (PMID: 24920887) ; Cell Physiol Biochem. 2014;33(6):1715-32. (PMID: 24923288) ; Anesthesiology. 2008 Aug;109(2):339-48. (PMID: 18648242) ; Arch Biochem Biophys. 2014 Mar 1;545:74-82. (PMID: 24412777) ; Exp Neurol. 2008 Aug;212(2):275-84. (PMID: 18511046) ; Acta Pharmacol Sin. 2010 Mar;31(3):273-80. (PMID: 20140005) ; Genes Dev. 2004 Jun 15;18(12):1466-81. (PMID: 15198984) ; Biochem Biophys Res Commun. 2008 Nov 14;376(2):404-8. (PMID: 18789310) ; J Ethnopharmacol. 2008 Jun 19;118(1):140-7. (PMID: 18486376) ; Cardiovasc Res. 2013 Jun 1;98(3):391-401. (PMID: 23447644) ; Oncol Rep. 2014 Jan;31(1):13-8. (PMID: 24173532) ; Pharmacol Biochem Behav. 2010 Sep;96(3):301-5. (PMID: 20566405) ; Int Immunopharmacol. 2010 Jun;10(6):668-78. (PMID: 20347053) ; Nature. 2013 Apr 11;496(7444):238-42. (PMID: 23535595) ; Neurochem Res. 2016 Mar;41(3):515-22. (PMID: 26108712) Contributed Indexing: Keywords: Heat shock protein; cell viability; neuron-specific enolase Substance Nomenclature: 0 (Chaperonin 60) ; 0 (Flavonoids) ; 0 (HSP70 Heat-Shock Proteins) ; 0 (Hypoxia-Inducible Factor 1, alpha Subunit) ; 0 (Neuroprotective Agents) ; EC 4.2.1.11 (Phosphopyruvate Hydratase) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20170201 Date Completed: 20170306 Latest Revision: 20181113 Update Code: 20231215 PubMed Central ID: PMC6130580

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The effects of icariin on the expression of HIF-1α, HSP-60 and HSP-70 in PC12 cells suffered from oxygen-glucose deprivation-induced injury.