Icariin promotes osteogenic differentiation of rat bone marrow stromal cells by activating the ERα-Wnt/β-catenin signaling pathway.

Wei, Q ; Zhang, J ; et al.

In: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jg. 84 (2016-12-01), S. 931

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Icariin, the main effective component of Herba Epimedii, has been identified to regulate the osteogenic differentiation of rat bone marrow stromal cells (rBMSCs) and have potential application in preventing bone loss and promoting bone regeneration. However, the underlying signaling pathway of its osteogenic effect remains unclear. Here, we investigated whether icariin could promote osteogenesis of rBMSCs through modulating the estrogen receptor alpha (ERα) and Wnt/β-catenin signaling pathways, which implicated in rBMSCs osteogenesis. rBMSCs were cultured in osteogenic induction medium and treated with icariin or together with ICI 182780 or DKK1, the effects on the expression of osteogenic genes and Wnt/β-catenin signaling were detected. Results indicated that icariin (0.1μM) markedly enhanced the proliferation of rBMSCs and alkaline phosphatase (ALP) activity. Additionally, icariin (0.1μM) significantly up-regulated the expression of osteogenic genes (Runx2, osteopotin, DLX5, osteocalcin, collagen type I, and ERα) and Wnt signal members (β-catenin, Lef1, TCF7, c-jun, c-myc, and cyclin D). Furthermore, icariin stimulated the activation of β-catenin as evidenced by increased total β-catenin protein and nuclear translocation. These osteogenesis-potentiating effects of icariin were blocked by ICI 182780 or DKK1. Taken together, these results suggest that the osteogenic effects of icariin on rBMSCs involves the ERα-Wnt/β-catenin signaling pathway.

Titel:	Icariin promotes osteogenic differentiation of rat bone marrow stromal cells by activating the ERα-Wnt/β-catenin signaling pathway.
Autor/in / Beteiligte Person:	Wei, Q ; Zhang, J ; Hong, G ; Chen, Z ; Deng, W ; He, W ; Chen, MH
Link:	Zum Volltext
Zeitschrift:	Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jg. 84 (2016-12-01), S. 931
Veröffentlichung:	Paris : Editions Scientifiques Elsevier ; <i>Original Publication</i>: New York, N.Y. : Masson Pub. USA, Inc., c1982-, 2016
Medientyp:	academicJournal
ISSN:	1950-6007 (electronic)
DOI:	10.1016/j.biopha.2016.09.107
Schlagwort:	Animals Cell Differentiation genetics Cell Nucleus drug effects Cell Nucleus metabolism Cell Proliferation drug effects Cell Shape drug effects Cells, Cultured Estradiol analogs & derivatives Estradiol pharmacology Fulvestrant Gene Expression Regulation drug effects Humans Intercellular Signaling Peptides and Proteins pharmacology Male Mesenchymal Stem Cells drug effects Mesenchymal Stem Cells metabolism Osteogenesis genetics Phenotype Phosphorylation drug effects Protein Transport drug effects Rats, Sprague-Dawley Wnt Signaling Pathway genetics beta Catenin metabolism Cell Differentiation drug effects Estrogen Receptor alpha metabolism Flavonoids pharmacology Mesenchymal Stem Cells cytology Osteogenesis drug effects Wnt Signaling Pathway drug effects
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Biomed Pharmacother] 2016 Dec; Vol. 84, pp. 931-939. <i>Date of Electronic Publication: </i>2016 Oct 17. MeSH Terms: Cell Differentiation / drug effects ; Estrogen Receptor alpha / metabolism ; Flavonoids / pharmacology ; Mesenchymal Stem Cells / cytology ; Osteogenesis / drug effects ; Wnt Signaling Pathway / drug effects ; Animals ; Cell Differentiation / genetics ; Cell Nucleus / drug effects ; Cell Nucleus / metabolism ; Cell Proliferation / drug effects ; Cell Shape / drug effects ; Cells, Cultured ; Estradiol / analogs & derivatives ; Estradiol / pharmacology ; Fulvestrant ; Gene Expression Regulation / drug effects ; Humans ; Intercellular Signaling Peptides and Proteins / pharmacology ; Male ; Mesenchymal Stem Cells / drug effects ; Mesenchymal Stem Cells / metabolism ; Osteogenesis / genetics ; Phenotype ; Phosphorylation / drug effects ; Protein Transport / drug effects ; Rats, Sprague-Dawley ; Wnt Signaling Pathway / genetics ; beta Catenin / metabolism Contributed Indexing: Keywords: Bone marrow stromal cells; Icariin; Osteogenic differentiation; Wnt/β-catenin signaling Substance Nomenclature: 0 (DKK1 protein, human) ; 0 (Estrogen Receptor alpha) ; 0 (Flavonoids) ; 0 (Intercellular Signaling Peptides and Proteins) ; 0 (beta Catenin) ; 22X328QOC4 (Fulvestrant) ; 4TI98Z838E (Estradiol) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20161021 Date Completed: 20170213 Latest Revision: 20220330 Update Code: 20231215

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