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Ethnopharmacological Relevance: Icariin, a principal flavonoid glycoside of Epimedium brevicornu Maxim, has been widely proved to possess antiosteoporotic activity with promoting bone formation and decreasing bone resorption. However, the involving mechanisms remain unclear.

Aim of the Study: To clear a global insight of signal pathways involved in anti-osteoporotic mechanism of icariin at proteins and metabolites level by integrating the proteomics and NMR metabonomics, in a systems biology approach.

Material and Methods: Mice were divided into sham, OVX model and icariin-treated OVX group, after 90 days treatment, difference gel electrophoresis combined with MALDI-TOF/TOF proteomics analysis on bone femur and serum metabolomics were carried out for monitor intracellular processes and elucidate anti-osteoporotic mechanism of icariin. Osteoblast and osteoclast were applied to evaluate the potential signal pathways.

Results: Twenty three proteins in bone femur, and 8 metabolites in serum, were significantly altered and identified, involving in bone remodeling, energy metabolism, cytoskeleton, lipid metabolism, MAPK signaling, Ca 2+ signaling et, al. Furthermore, animal experiment show icariin could enhance the BMD and BMC, decrease CTX-I level in ovariectomized mice. The mitochondrial membrane potential and the intracellular ATP levels were increased significantly, and the cytoskeleton were improved in icariin-treatment osteoblast and osteoclast. Icariin also increased mRNA expression of Runx2 and osterix of OB, decreased CTR and CAII mRNA expression and protein expression of P38 and JNK. However, icariin did not reveal any inhibition of the collagenolytic activity of cathepsin K, mRNA expression of MMP-9 and protein expression of ERK in osteoclast.

Conclusion: we consider icariin as multi-targeting compounds for treating with osteoporosis, involve initiating osteoblastogenesis, inhibiting adipogenesis, and preventing osteoclast differentiation.

Titel:	Comparative proteomic and metabolomic analysis reveal the antiosteoporotic molecular mechanism of icariin from Epimedium brevicornu maxim.
Autor/in / Beteiligte Person:	Xue, L ; Jiang, Y ; Han, T ; Zhang, N ; Qin, L ; Xin, H ; Zhang, Q
Link:	Zum Volltext
Zeitschrift:	Journal of ethnopharmacology, Jg. 192 (2016-11-04), S. 370
Veröffentlichung:	Limerick : Elsevier Sequoia ; <i>Original Publication</i>: Lausanne, Elsevier Sequoia., 2016
Medientyp:	academicJournal
ISSN:	1872-7573 (electronic)
DOI:	10.1016/j.jep.2016.07.037
Schlagwort:	Adipogenesis drug effects Animals Biomarkers blood Bone Density drug effects Bone Density Conservation Agents isolation & purification Cell Differentiation drug effects Cells, Cultured Disease Models, Animal Electrophoresis, Gel, Two-Dimensional Female Femur metabolism Flavonoids isolation & purification Gene Expression Regulation drug effects Mice, Inbred ICR Osteoblasts drug effects Osteoblasts metabolism Osteoclasts drug effects Osteoclasts metabolism Osteoporosis blood Osteoporosis genetics Phytotherapy Plant Extracts isolation & purification Plants, Medicinal Proton Magnetic Resonance Spectroscopy RNA, Messenger genetics RNA, Messenger metabolism Signal Transduction drug effects Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Bone Density Conservation Agents pharmacology Bone Remodeling drug effects Epimedium chemistry Femur drug effects Flavonoids pharmacology Metabolomics methods Osteoporosis drug therapy Plant Extracts pharmacology Proteomics methods
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Comparative Study; Journal Article Language: English [J Ethnopharmacol] 2016 Nov 04; Vol. 192, pp. 370-381. <i>Date of Electronic Publication: </i>2016 Jul 12. MeSH Terms: Metabolomics* / methods ; Proteomics* / methods ; Bone Density Conservation Agents / pharmacology ; Bone Remodeling / drug effects ; Epimedium / chemistry ; Femur / drug effects ; Flavonoids / pharmacology ; Osteoporosis / drug therapy ; Plant Extracts / *pharmacology ; Adipogenesis / drug effects ; Animals ; Biomarkers / blood ; Bone Density / drug effects ; Bone Density Conservation Agents / isolation & purification ; Cell Differentiation / drug effects ; Cells, Cultured ; Disease Models, Animal ; Electrophoresis, Gel, Two-Dimensional ; Female ; Femur / metabolism ; Flavonoids / isolation & purification ; Gene Expression Regulation / drug effects ; Mice, Inbred ICR ; Osteoblasts / drug effects ; Osteoblasts / metabolism ; Osteoclasts / drug effects ; Osteoclasts / metabolism ; Osteoporosis / blood ; Osteoporosis / genetics ; Phytotherapy ; Plant Extracts / isolation & purification ; Plants, Medicinal ; Proton Magnetic Resonance Spectroscopy ; RNA, Messenger / genetics ; RNA, Messenger / metabolism ; Signal Transduction / drug effects ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Contributed Indexing: Keywords: Bone metabolism; Metabolomics; Osteoporosis; Proteomics Substance Nomenclature: 0 (Biomarkers) ; 0 (Bone Density Conservation Agents) ; 0 (Flavonoids) ; 0 (Plant Extracts) ; 0 (RNA, Messenger) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20160717 Date Completed: 20170515 Latest Revision: 20211122 Update Code: 20240513

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Comparative proteomic and metabolomic analysis reveal the antiosteoporotic molecular mechanism of icariin from Epimedium brevicornu maxim.