Einzeltreffer — DigiBib

Ethnopharmacological Relevance: Crateva adansonii DC is a plant traditionally used in Cameroon to treat constipation, asthma, snakebites, postmenopausal complaints and cancers.

Aim: The anticancer potential of the dichloromethane/methanol extract of C. adansonii stem barks was investigated using human breast cancer cell and 7,12 dimethylbenz(a)anththracene (DMBA)-induced mammary tumorigenesis model in rats.

Material and Methods: The cytotoxicity of C. adansonii extract was assessed in vitro towards breast carcinoma (MCF-7 and MDA-MB-231) and non-tumoral cell lines (NIH/3T3 and HUVEC) by Alamar Blue assay. Furthermore, in vivo studies were performed on female Wistar rats treated either with C. adansonii extract at a dose of 75 or 300mg/kg body weight or with tamoxifen (3.3mg/kg body weight), starting 1 week prior DMBA treatment and lasted 12 weeks. The investigation focused on tumour burden, tumour DNA fingerprint, morphological, histological, hematological, and biochemical parameters.

Results: CC50 values for the in vitro assays were 289µg/mL against MCF-7 cells and >500µg/mL in others cells, leading to a selectivity index ≥1.73. C. adansonii extract significantly (p<0.001) revealed in vivo the reduction of the cumulative tumour yield (87.23%), total tumour burden (88.64%), average tumour weight (71.11%) and tumour volume (78.07%) at the dose of 75mg/kg as compared to DMBA control group. A weak effect was also observed at 300mg/kg. This extract showed a moderate hyperplasia at the dose of 75mg/kg while at 300mg/kg no significant change was noted as compared to DMBA group. It protected rats from the DNA alteration induced by DMBA and increased antioxydant enzymes activities in mammary gland tissue homogenates. In addition, Ultra-High Performance Liquid Chromatography/ESI-QTOF-Mass Spectrometry analysis of C. adansonii extract detected structure-related of many well-known anticancer agents such as flavane gallate, flavonol, phenylpropanoïds, sesquiterpene derivatives, gallotannins and lignans. The LD50 of C. adansonii was estimated to be greater than 5000mg/kg.

Conclusions: These aforementioned results suggest that the C. adansonii extract may possess antitumor constituents, which could combat breast cancer and prevent chemically-induced breast cancer in rats.

Titel:	Crateva adansonii DC, an African ethnomedicinal plant, exerts cytotoxicity in vitro and prevents experimental mammary tumorigenesis in vivo.
Autor/in / Beteiligte Person:	Zingue, S ; Cisilotto, J ; Tueche, AB ; Bishayee, A ; Mefegue, FA ; Sandjo, LP ; Magne Nde, CB ; Winter, E ; Michel, T ; Ndinteh, DT ; Awounfack, CF ; Silihe, KK ; Melachio Tanekou, TT ; Creczynski-Pasa, TB ; Njamen, D
Link:	Zum Volltext
Zeitschrift:	Journal of ethnopharmacology, Jg. 190 (2016-08-22), S. 183
Veröffentlichung:	Limerick : Elsevier Sequoia ; <i>Original Publication</i>: Lausanne, Elsevier Sequoia., 2016
Medientyp:	academicJournal
ISSN:	1872-7573 (electronic)
DOI:	10.1016/j.jep.2016.06.004
Schlagwort:	9,10-Dimethyl-1,2-benzanthracene Africa Animals Anticarcinogenic Agents chemistry Anticarcinogenic Agents isolation & purification Anticarcinogenic Agents toxicity Antineoplastic Agents, Phytogenic chemistry Antineoplastic Agents, Phytogenic isolation & purification Antineoplastic Agents, Phytogenic toxicity Breast Neoplasms genetics Breast Neoplasms metabolism Breast Neoplasms pathology Chromatography, Liquid DNA Damage drug effects Dose-Response Relationship, Drug Ethnobotany Female Human Umbilical Vein Endothelial Cells drug effects Human Umbilical Vein Endothelial Cells pathology Humans Inhibitory Concentration 50 Lethal Dose 50 MCF-7 Cells Mammary Neoplasms, Experimental chemically induced Mammary Neoplasms, Experimental pathology Medicine, African Traditional Mice Molecular Structure NIH 3T3 Cells Oxidative Stress drug effects Phytotherapy Plant Extracts chemistry Plant Extracts isolation & purification Plant Extracts toxicity Plants, Medicinal Rats, Wistar Spectrometry, Mass, Electrospray Ionization Tamoxifen pharmacology Time Factors Tumor Burden drug effects Anticarcinogenic Agents pharmacology Antineoplastic Agents, Phytogenic pharmacology Breast Neoplasms drug therapy Capparaceae chemistry Mammary Neoplasms, Experimental prevention & control Plant Extracts pharmacology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Comparative Study; Journal Article Language: English [J Ethnopharmacol] 2016 Aug 22; Vol. 190, pp. 183-99. <i>Date of Electronic Publication: </i>2016 Jun 04. MeSH Terms: Anticarcinogenic Agents / pharmacology ; Antineoplastic Agents, Phytogenic / pharmacology ; Breast Neoplasms / drug therapy ; Capparaceae / chemistry ; Mammary Neoplasms, Experimental / prevention & control ; Plant Extracts / pharmacology ; 9,10-Dimethyl-1,2-benzanthracene ; Africa ; Animals ; Anticarcinogenic Agents / chemistry ; Anticarcinogenic Agents / isolation & purification ; Anticarcinogenic Agents / toxicity ; Antineoplastic Agents, Phytogenic / chemistry ; Antineoplastic Agents, Phytogenic / isolation & purification ; Antineoplastic Agents, Phytogenic / toxicity ; Breast Neoplasms / genetics ; Breast Neoplasms / metabolism ; Breast Neoplasms / pathology ; Chromatography, Liquid ; DNA Damage / drug effects ; Dose-Response Relationship, Drug ; Ethnobotany ; Female ; Human Umbilical Vein Endothelial Cells / drug effects ; Human Umbilical Vein Endothelial Cells / pathology ; Humans ; Inhibitory Concentration 50 ; Lethal Dose 50 ; MCF-7 Cells ; Mammary Neoplasms, Experimental / chemically induced ; Mammary Neoplasms, Experimental / pathology ; Medicine, African Traditional ; Mice ; Molecular Structure ; NIH 3T3 Cells ; Oxidative Stress / drug effects ; Phytotherapy ; Plant Extracts / chemistry ; Plant Extracts / isolation & purification ; Plant Extracts / toxicity ; Plants, Medicinal ; Rats, Wistar ; Spectrometry, Mass, Electrospray Ionization ; Tamoxifen / pharmacology ; Time Factors ; Tumor Burden / drug effects Contributed Indexing: Keywords: Antioxidant activity; Breast cancer; Chemoprevention; Crateva adansonii; Cytotoxicty; Dmba Substance Nomenclature: 0 (Anticarcinogenic Agents) ; 0 (Antineoplastic Agents, Phytogenic) ; 0 (Plant Extracts) ; 094ZI81Y45 (Tamoxifen) ; 57-97-6 (9,10-Dimethyl-1,2-benzanthracene) Entry Date(s): Date Created: 20160609 Date Completed: 20170418 Latest Revision: 20170418 Update Code: 20240513

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Crateva adansonii DC, an African ethnomedicinal plant, exerts cytotoxicity in vitro and prevents experimental mammary tumorigenesis in vivo.