[Transformation of icariin by immobilized β-glucosidase and snailase].
In: Yao xue xue bao = Acta pharmaceutica Sinica, Jg. 50 (2015-12-01), Heft 12, S. 1652
Online
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Zugriff:
This study was performed to prepare immobilized β-glucosidase and snailase, then optimize and compare the process conditions for conversion of icariin. Immobilized β-glucosidase and snailase were prepared using crosslink-embedding method. The best conditions of the preparation process were optimized by single factor analysis and the properties of immobilized β-glucosidase and snailase were investigated. The reaction conditions including temperature, pH, substrate ratio, substrate concentration, reaction time and reusing times of the conversion of icariin using immobilized β-glucosidase or snailase were optimized. Immobilized β-glucosidase and snailase exhibited better heat stabilities and could remain about 60% activity after storage at 4 degrees C for 4 weeks. The optimized conditions for the conversion of icariin were as follows, the temperature of 50 degrees C, pH of 5.0, enzyme and substrate ratio of 1 : 1, substrate concentration of 0.1 mg x mL(-1), reaction time of 6 h for β-glucosidase and 2 h for snailase, respectively. In 5 experiments, the average conversion ratio of immobilized β-glucosidase and snailase was 70.76% and 74.97%. The results suggest an effect of promoted stabilities, prolonged lifetimes in both β-glucosidase and snailase after immobilization. The immobilized β-glucosidase and snailase exhibited a higher conversion rate and reusability compared to the free β-glucosidase and snailase. Moreover, the conversion rate of immobilized snailase was higher than that of immobilized β-glucosidase. The process of icariin conversion using immobilized β-glucosidase and snailase was moderate and feasible, which suggests that immobilized enzymes may hold a promise for industrial usage.
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[Transformation of icariin by immobilized β-glucosidase and snailase].
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Autor/in / Beteiligte Person: | Peng, J ; Ma, YH ; Chen, Y ; Liu, CY ; Gao, X ; Zhou, J |
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Zeitschrift: | Yao xue xue bao = Acta pharmaceutica Sinica, Jg. 50 (2015-12-01), Heft 12, S. 1652 |
Veröffentlichung: | Beijing : Zhongguo yao xue hui, 2015 |
Medientyp: | academicJournal |
ISSN: | 0513-4870 (print) |
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