Coordinate regulatory osteogenesis effects of icariin, timosaponin B II and ferulic acid from traditional Chinese medicine formulas on UMR-106 osteoblastic cells and osteoblasts in neonatal rat calvaria cultures.

Li, M ; Zhang, ND ; et al.

In: Journal of ethnopharmacology, Jg. 185 (2016-06-05), S. 120

Online academicJournal

Zugriff:

Zum Volltext

Ethnopharmacological Significance: Icariin (I), ferulic acid (F) and timosaponin B II (T) derived respectively from the leaf of Epimedium brevicornu Maxim (EBM, Berberidaceae), rhizome of Anemarrhena asphodeloides Bunge (AAB, Liliaceae) and root of Angelica sinensis (Oliv.) Diels (ASD, Umbelliferae) are included in several traditional Chinese medicine (TCM) formulas for the treatment of osteoporosis. In addition, the medicinal materials and chemical constituents in many traditional Chinese formulas have been shown to have potential synergistic, additive and antagonistic effects.

Aim of Study: To explore the action mechanism and interactions between I, T and F as bone anabolic ingredients on osteoblasts, and fully understand their action mechanism and rationality of the formula design.

Materials and Methods: An osteoporotic model was established in bilaterally ovariectomized mice. Bone mineral density (BMD), bone mineral content (BMC) and serum biochemical parameters including alkaline phosphatase (ALP), tartrate resistant acid phosphatase (TRAP), osteoprotegerin (OPG) and deoxypyridinoline cross-links (DPD) were measured to evaluate the effects of I, T or F alone and their combinations on osteoporotic mice. UMR-106 osteoblastic cells and primary osteoblasts in neonatal rat calvarias were used to evaluate the osteogenesis effect. The immunohistochemical method and Western-blot analysis were used to detect the expression of critical proteins in the process of proliferation and differentiation of osteoblasts.

Results: IFT combinations enhanced the therapeutic effect without increasing the adverse effects on osteoporotic mice, synergistically increased the osteoblast proliferation, ALP activity and mineralized nodule formation, and promoted the expression of bone matrix by regulating BMP and Wnt/β-catenin signaling pathways in osteoblasts.

Conclusion: IFT combinations reinforced the therapeutic effect on osteoporosis by modulating multi-signaling pathways and action targets.

Titel:	Coordinate regulatory osteogenesis effects of icariin, timosaponin B II and ferulic acid from traditional Chinese medicine formulas on UMR-106 osteoblastic cells and osteoblasts in neonatal rat calvaria cultures.
Autor/in / Beteiligte Person:	Li, M ; Zhang, ND ; Wang, Y ; Han, T ; Jiang, YP ; Rahman, K ; Qin, LP ; Xin, HL ; Zhang, QY
Link:	Zum Volltext
Zeitschrift:	Journal of ethnopharmacology, Jg. 185 (2016-06-05), S. 120
Veröffentlichung:	Limerick : Elsevier Sequoia ; <i>Original Publication</i>: Lausanne, Elsevier Sequoia., 2016
Medientyp:	academicJournal
ISSN:	1872-7573 (electronic)
DOI:	10.1016/j.jep.2016.03.023
Schlagwort:	Animals Animals, Newborn Calcium metabolism Cell Line Cell Proliferation drug effects Coumaric Acids administration & dosage Coumaric Acids chemistry Female Flavonoids administration & dosage Flavonoids chemistry Gene Expression Regulation Mice Molecular Structure Osteoblasts metabolism Osteoporosis drug therapy Rats Saponins administration & dosage Saponins chemistry Steroids administration & dosage Steroids chemistry Coumaric Acids pharmacology Drugs, Chinese Herbal chemistry Flavonoids pharmacology Osteoblasts drug effects Osteogenesis drug effects Saponins pharmacology Steroids pharmacology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [J Ethnopharmacol] 2016 Jun 05; Vol. 185, pp. 120-31. <i>Date of Electronic Publication: </i>2016 Mar 13. MeSH Terms: Coumaric Acids / pharmacology ; Drugs, Chinese Herbal / chemistry ; Flavonoids / pharmacology ; Osteoblasts / drug effects ; Osteogenesis / drug effects ; Saponins / pharmacology ; Steroids / *pharmacology ; Animals ; Animals, Newborn ; Calcium / metabolism ; Cell Line ; Cell Proliferation / drug effects ; Coumaric Acids / administration & dosage ; Coumaric Acids / chemistry ; Female ; Flavonoids / administration & dosage ; Flavonoids / chemistry ; Gene Expression Regulation ; Mice ; Molecular Structure ; Osteoblasts / metabolism ; Osteoporosis / drug therapy ; Rats ; Saponins / administration & dosage ; Saponins / chemistry ; Steroids / administration & dosage ; Steroids / chemistry Contributed Indexing: Keywords: BMP signaling pathway; Ferulic acid; Ferulic acid (Pubchem CID: 445858); Iacriin (Pubchem CID: 5318997); Icariin; Osteogenic differentiation; Osteoporosis; Timosaponin B II; Timosaponin B II (Pubchem CID: 44575945); Traditional Chinese medicine formula; Wnt/β-Catenin signaling pathway Substance Nomenclature: 0 (Coumaric Acids) ; 0 (Drugs, Chinese Herbal) ; 0 (Flavonoids) ; 0 (Saponins) ; 0 (Steroids) ; 0 (timosaponin B-II) ; AVM951ZWST (ferulic acid) ; SY7Q814VUP (Calcium) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20160318 Date Completed: 20170224 Latest Revision: 20170224 Update Code: 20231215

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.