blaCTX-M-15 carried by IncF-type plasmids is the dominant ESBL gene in Escherichia coli and Klebsiella pneumoniae at a hospital in Ghana.

Agyekum, A ; Fajardo-Lubián, A ; et al.

In: Diagnostic microbiology and infectious disease, Jg. 84 (2016-04-01), Heft 4, S. 328

Online academicJournal

Zugriff:

Zum Volltext

Escherichia coli and Klebsiella pneumoniae producing extended-spectrum β-lactamases (ESBLs) are among the most multidrug-resistant pathogens in hospitals and are spreading worldwide. Horizontal gene transfer and spread of high-risk clones are involved in ESBL dissemination. Investigation of the resistance phenotypes of 101 consecutive clinical E. coli (n=58) and K. pneumoniae (n=43) isolated at the Komfo Anokye Teaching Hospital in Ghana over 3 months revealed 63 (62%) with an ESBL phenotype. All 63 had a blaCTX-M gene, and sequence analysis showed that 62 of these were blaCTX-M-15. blaCTX-M-15 was linked to ISEcp1 and orf477Δ in all isolates, and most isolates also carried blaTEM, aac(3)-II, aacA4cr, and/or blaOXA-30 genes on IncF plasmids. XbaI/pulsed-field electrophoresis showed heterogeneity among isolates of both species, suggesting that blaCTX-M-15 dissemination is caused by horizontal gene transfer rather than clonal spread of these species in Ghana.

Titel:	blaCTX-M-15 carried by IncF-type plasmids is the dominant ESBL gene in Escherichia coli and Klebsiella pneumoniae at a hospital in Ghana.
Autor/in / Beteiligte Person:	Agyekum, A ; Fajardo-Lubián, A ; Ansong, D ; Partridge, SR ; Agbenyega, T ; Iredell, JR
Link:	Zum Volltext
Zeitschrift:	Diagnostic microbiology and infectious disease, Jg. 84 (2016-04-01), Heft 4, S. 328
Veröffentlichung:	[New York, NY] : Elsevier Biomedical, [c1983-, 2016
Medientyp:	academicJournal
ISSN:	1879-0070 (electronic)
DOI:	10.1016/j.diagmicrobio.2015.12.010
Schlagwort:	Electrophoresis, Gel, Pulsed-Field Escherichia coli classification Escherichia coli genetics Escherichia coli isolation & purification Gene Transfer, Horizontal Genes, Bacterial Genetic Variation Ghana Hospitals Humans Klebsiella pneumoniae classification Klebsiella pneumoniae genetics Klebsiella pneumoniae isolation & purification Molecular Typing Plasmids classification Sequence Analysis, DNA Escherichia coli enzymology Escherichia coli Infections microbiology Klebsiella Infections microbiology Klebsiella pneumoniae enzymology Plasmids isolation & purification beta-Lactamases genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Diagn Microbiol Infect Dis] 2016 Apr; Vol. 84 (4), pp. 328-33. <i>Date of Electronic Publication: </i>2015 Dec 17. MeSH Terms: Escherichia coli / enzymology ; Escherichia coli Infections / microbiology ; Klebsiella Infections / microbiology ; Klebsiella pneumoniae / enzymology ; Plasmids / isolation & purification ; beta-Lactamases / genetics ; Electrophoresis, Gel, Pulsed-Field ; Escherichia coli / classification ; Escherichia coli / genetics ; Escherichia coli / isolation & purification ; Gene Transfer, Horizontal ; Genes, Bacterial ; Genetic Variation ; Ghana ; Hospitals ; Humans ; Klebsiella pneumoniae / classification ; Klebsiella pneumoniae / genetics ; Klebsiella pneumoniae / isolation & purification ; Molecular Typing ; Plasmids / classification ; Sequence Analysis, DNA Contributed Indexing: Keywords: Antibiotic resistance; CTX-M enzymes; ESBLs; Enterobacteriaceae; Ghana; PFGE Substance Nomenclature: EC 3.5.2.- (beta-lactamase CTX-M-15) ; EC 3.5.2.6 (beta-Lactamases) Entry Date(s): Date Created: 20160203 Date Completed: 20161213 Latest Revision: 20201209 Update Code: 20240513

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.