Einzeltreffer — DigiBib

Biosynthesis of aspartate (Asp)-derived amino acids lysine (Lys), methionine (Met), threonine (Thr), and isoleucine involves monofunctional Asp kinases (AKs) and dual-functional Asp kinase-homoserine dehydrogenases (AK-HSDHs). Four-week-old loss-of-function Arabidopsis (Arabidopsis thaliana) mutants in the AK-HSDH2 gene had increased amounts of Asp and Asp-derived amino acids, especially Thr, in leaves. To explore mechanisms behind this phenotype, we obtained single mutants for other AK and AK-HSDH genes, generated double mutants from ak-hsdh2 and ak mutants, and performed free and protein-bound amino acid profiling, transcript abundance, and activity assays. The increases of Asp, Lys, and Met in ak-hsdh2 were also observed in ak1-1, ak2-1, ak3-1, and ak-hsdh1-1. However, the Thr increase in ak-hsdh2 was observed in ak-hsdh1-1 but not in ak1-1, ak2-1, or ak3-1. Activity assays showed that AK2 and AK-HSDH1 are the major contributors to overall AK and HSDH activities, respectively. Pairwise correlation analysis revealed positive correlations between the amount of AK transcripts and Lys-sensitive AK activity and between the amount of AK-HSDH transcripts and both Thr-sensitive AK activity and total HSDH activity. In addition, the ratio of total AK activity to total HSDH activity negatively correlates with the ratio of Lys to the total amount of Met, Thr, and isoleucine. These data led to the hypothesis that the balance between Lys-sensitive AKs and Thr-sensitive AK-HSDHs is important for maintaining the amounts and ratios of Asp-derived amino acids.

Titel:	Analysis of Loss-of-Function Mutants in Aspartate Kinase and Homoserine Dehydrogenase Genes Points to Complexity in the Regulation of Aspartate-Derived Amino Acid Contents.
Autor/in / Beteiligte Person:	Clark, TJ ; Lu, Y
Link:	Zum Volltext
Zeitschrift:	Plant physiology, Jg. 168 (2015-08-01), Heft 4, S. 1512
Veröffentlichung:	<May 2001->: [Rockville, MD] : American Society of Plant Biologists ; <i>Original Publication</i>: Lancaster, Pa., American Society of Plant Physiologists., 2015
Medientyp:	academicJournal
ISSN:	1532-2548 (electronic)
DOI:	10.1104/pp.15.00364
Schlagwort:	Amino Acids metabolism Arabidopsis genetics Arabidopsis metabolism Arabidopsis Proteins metabolism Aspartate Kinase metabolism Aspartic Acid metabolism Aspartokinase Homoserine Dehydrogenase metabolism Chromatography, High Pressure Liquid Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Immunoblotting Isoenzymes genetics Isoenzymes metabolism Lysine genetics Lysine metabolism Methionine genetics Methionine metabolism Plant Leaves genetics Plant Leaves metabolism Reverse Transcriptase Polymerase Chain Reaction Tandem Mass Spectrometry Threonine genetics Threonine metabolism Amino Acids genetics Arabidopsis Proteins genetics Aspartate Kinase genetics Aspartic Acid genetics Aspartokinase Homoserine Dehydrogenase genetics Mutation
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Language: English [Plant Physiol] 2015 Aug; Vol. 168 (4), pp. 1512-26. <i>Date of Electronic Publication: </i>2015 Jun 10. MeSH Terms: Mutation* ; Amino Acids / genetics ; Arabidopsis Proteins / genetics ; Aspartate Kinase / genetics ; Aspartic Acid / genetics ; Aspartokinase Homoserine Dehydrogenase / *genetics ; Amino Acids / metabolism ; Arabidopsis / genetics ; Arabidopsis / metabolism ; Arabidopsis Proteins / metabolism ; Aspartate Kinase / metabolism ; Aspartic Acid / metabolism ; Aspartokinase Homoserine Dehydrogenase / metabolism ; Chromatography, High Pressure Liquid ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Plant ; Immunoblotting ; Isoenzymes / genetics ; Isoenzymes / metabolism ; Lysine / genetics ; Lysine / metabolism ; Methionine / genetics ; Methionine / metabolism ; Plant Leaves / genetics ; Plant Leaves / metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Tandem Mass Spectrometry ; Threonine / genetics ; Threonine / metabolism References: J Biotechnol. 2007 Feb 1;128(2):335-43. (PMID: 17116342) ; Plant Mol Biol. 2005 Jan;57(2):241-54. (PMID: 15821880) ; Anal Biochem. 1968 Mar;22(3):536-9. (PMID: 5672501) ; Amino Acids. 2006 Mar;30(2):127-42. (PMID: 16547652) ; Protein Expr Purif. 2002 Feb;24(1):99-104. (PMID: 11812229) ; Genes Genet Syst. 2001 Jun;76(3):189-98. (PMID: 11569502) ; Nahrung. 1998 Aug;42(3-4):125-7. (PMID: 9739551) ; Plant Physiol. 1990 May;93(1):98-104. (PMID: 16667473) ; Biochim Biophys Acta. 1969 Feb 11;171(2):205-16. (PMID: 4388077) ; Plant J. 2000 Nov;24(3):357-67. (PMID: 11069709) ; Mol Syst Biol. 2009;5:271. (PMID: 19455135) ; Plant Physiol. 2010 Feb;152(2):529-40. (PMID: 19906890) ; Plant J. 2004 Aug;39(3):465-75. (PMID: 15255874) ; Funct Plant Biol. 2012 Dec;39(12):1058-1067. (PMID: 32480855) ; Plant Physiol. 2008 Apr;146(4):1482-500. (PMID: 18263779) ; J Plant Res. 2007 Jan;120(1):157-65. (PMID: 17186119) ; FEBS Lett. 2000 Dec 29;487(2):234-8. (PMID: 11150516) ; Plant Physiol. 1995 Jan;107(1):43-52. (PMID: 12228340) ; Nat Genet. 2005 May;37(5):501-6. (PMID: 15806101) ; Physiol Plant. 2013 Dec;149(4):449-60. (PMID: 23556418) ; Plant Physiol. 1975 Jun;55(6):991-8. (PMID: 16659233) ; J Exp Bot. 2007;58(10):2653-60. (PMID: 17545223) ; Plant Cell. 2011 May;23(5):1861-75. (PMID: 21586683) ; Plant Physiol. 1994 Dec;106(4):1303-12. (PMID: 7846152) ; Plant J. 2010 Mar;61(6):928-40. (PMID: 20409268) ; FEBS J. 2007 Jan;274(1):164-76. (PMID: 17140415) ; Plant Physiol. 2001 Aug;126(4):1539-45. (PMID: 11500552) ; Science. 2003 Aug 1;301(5633):653-7. (PMID: 12893945) ; Arabidopsis Book. 2009;7:e0121. (PMID: 22303247) ; Plant Mol Biol. 1997 May;34(2):287-93. (PMID: 9207844) ; Plant Physiol. 2008 Jul;147(3):954-61. (PMID: 18612072) ; Biochem Genet. 1997 Apr;35(3-4):139-53. (PMID: 9241437) ; J Biol Chem. 2003 Feb 14;278(7):5361-6. (PMID: 12435751) ; Plant J. 2010 Feb;61(4):579-90. (PMID: 19929878) ; PLoS One. 2007 Aug 08;2(8):e718. (PMID: 17684564) ; J Exp Bot. 2012 Oct;63(16):5991-6001. (PMID: 23048130) ; Proc Natl Acad Sci U S A. 2006 Oct 17;103(42):15687-92. (PMID: 17030798) ; Plant Mol Biol. 2003 Jan;51(2):281-94. (PMID: 12602885) ; Plant Physiol. 1998 Mar;116(3):1023-8. (PMID: 9501134) ; Curr Opin Struct Biol. 2001 Dec;11(6):694-700. (PMID: 11751050) ; Plant Mol Biol. 1997 May;34(2):233-42. (PMID: 9207839) ; Plant Physiol. 2006 Nov;142(3):878-89. (PMID: 16980562) ; Plant Physiol. 2011 Apr;155(4):1589-600. (PMID: 21224340) ; Nucleic Acids Res. 2012 Mar;40(6):2454-69. (PMID: 22127866) ; Plant Physiol. 2005 Jun;138(2):990-7. (PMID: 15908595) ; BMC Bioinformatics. 2012 Jan 13;13:10. (PMID: 22244038) ; Methods Mol Biol. 2012;828:1-11. (PMID: 22125131) ; J Biol Chem. 2005 Dec 16;280(50):41178-83. (PMID: 16216875) ; Arabidopsis Book. 2010;8:e0137. (PMID: 22303262) ; Plant Physiol. 1989 Dec;91(4):1602-8. (PMID: 16667223) ; Methods Mol Biol. 2011;775:161-85. (PMID: 21863443) ; Planta. 2011 May;233(5):1025-40. (PMID: 21279647) Substance Nomenclature: 0 (Amino Acids) ; 0 (Arabidopsis Proteins) ; 0 (Isoenzymes) ; 2ZD004190S (Threonine) ; 30KYC7MIAI (Aspartic Acid) ; AE28F7PNPL (Methionine) ; EC 2.7.2.4 (Aspartate Kinase) ; EC 2.7.2.4 (Aspartokinase Homoserine Dehydrogenase) ; K3Z4F929H6 (Lysine) Entry Date(s): Date Created: 20150612 Date Completed: 20160511 Latest Revision: 20240323 Update Code: 20240323 PubMed Central ID: PMC4528744

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Analysis of Loss-of-Function Mutants in Aspartate Kinase and Homoserine Dehydrogenase Genes Points to Complexity in the Regulation of Aspartate-Derived Amino Acid Contents.