The method of integrated kinetics and its applicability to the exo-glycosidase-catalyzed hydrolysis of p-nitrophenyl glycosides.
In: Carbohydrate research, Jg. 412 (2015-08-14), S. 43
Online
academicJournal
Zugriff:
In the present work we suggest an efficient method, using the whole time course of the reaction, whereby parameters kcat, Km and product KI for the hydrolysis of a p-nitrophenyl glycoside by an exo-acting glycoside hydrolase can be estimated in a single experiment. Its applicability was demonstrated for three retaining exo-glycoside hydrolases, β-xylosidase from Aspergillus awamori, β-galactosidase from Penicillium sp. and α-galactosidase from Thermotoga maritima (TmGalA). During the analysis of the reaction course catalyzed by the TmGalA enzyme we had observed that a non-enzymatic process, mutarotation of the liberated α-d-galactose, affected the reaction significantly.
(Copyright © 2015 Elsevier Ltd. All rights reserved.)
| Titel: |
The method of integrated kinetics and its applicability to the exo-glycosidase-catalyzed hydrolysis of p-nitrophenyl glycosides.
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| Autor/in / Beteiligte Person: | Borisova, AS ; Reddy, SK ; Ivanen, DR ; Bobrov, KS ; Eneyskaya, EV ; Rychkov, GN ; Sandgren, M ; Stålbrand, H ; Sinnott, ML ; Kulminskaya, AA ; Shabalin, KA |
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| Zeitschrift: | Carbohydrate research, Jg. 412 (2015-08-14), S. 43 |
| Veröffentlichung: | Amsterdam : Elsevier ; <i>Original Publication</i>: Amsterdam., 2015 |
| Medientyp: | academicJournal |
| ISSN: | 1873-426X (electronic) |
| DOI: | 10.1016/j.carres.2015.03.021 |
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