Simultaneous determination of icariin, naringin and osthole in rat plasma by UPLC-MS/MS and its application for pharmacokinetic study after oral administration of Gushudan capsules.
In: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, Jg. 993-994 (2015-07-01), S. 75
Online
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Zugriff:
A rapid, sensitive and selective ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous determination of icariin, naringin and osthole in rat plasma. Plasma samples pretreatment involved a one-step liquid-liquid extraction with a mixture of ethyl acetate-methyl tert-butyl ether (3:1, ν/ν). The separation was performed on an ACQUITY UPLC™ BEH C18 column with a gradient mobile phase system of methanol and water. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization (ESI) by multiple reactions monitoring (MRM), with the transitions at m/z 513.3→366.8 (icariin), m/z 579.3→150.9 (naringin), m/z 245.1→189.0 (osthole) and m/z 237.1→194.1 (IS), respectively. A good linear response was observed over the concentration ranges of 1.06-424ng/ml, 2.10-525ng/ml and 1.05-1.05×10(3)ng/ml with lower limit of quantification (LLOQ) of 1.06, 2.10 and 1.05ng/ml for icariin, naringin and osthole, respectively. The intra- and inter-day precisions (R.S.D.) were within 14.3%, and the accuracy (R.E.) ranged from -4.1% to 4.6% at three quality control levels. The sensitive and selective method was applied to a pharmacokinetic study of icarrin, naringin and osthole in rats after oral administration of Gushudan capsule.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Titel: |
Simultaneous determination of icariin, naringin and osthole in rat plasma by UPLC-MS/MS and its application for pharmacokinetic study after oral administration of Gushudan capsules.
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Autor/in / Beteiligte Person: | Lu, Y ; Li, N ; Deng, Y ; Zhao, L ; Guo, X ; Li, F ; Xiong, Z |
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Zeitschrift: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, Jg. 993-994 (2015-07-01), S. 75 |
Veröffentlichung: | Amsterdam ; New York : Elsevier, c2002-, 2015 |
Medientyp: | academicJournal |
ISSN: | 1873-376X (electronic) |
DOI: | 10.1016/j.jchromb.2015.04.021 |
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