Exercises in hot and humid environment caused liver injury in a rat model.
In: PloS one, Jg. 9 (2014-12-30), Heft 12, S. e111741
Online
academicJournal
Zugriff:
Objective: To investigate injury pattern during intense exercises in hot and humid environment particularly on liver in a rat exertional heat stroke model.
Methods: We randomly divided 30 rats into a control group (CG), a normal temperature (25±2°C, 60%±5% humidity) exercise group (NTEG) and a high temperature and high humidity (35±2°C, 80%±10% humidity) exercising group (HTEG), each comprising 10 animals. The NTEG and HTEG rats were forced to run in a treadmill for 1 hour maximum at 20 rpm. We analyzed liver cells of all three groups with JC-1 dye and flow cytometry for apoptosis rates in addition to liver tissue 8 - hydroxy deoxyguanosine (8 - OhdG) and blood serum IL-6, tumor necrosis factor alpha (TNF-α), alanine aminotransferase ALT, aspartate amino transferase (AST), serum creatinine (CREA), blood urea nitrogen (BUN), lactate dehydrogenase (LDH), creatine phosphate kinase (CK) concentrations.
Result: Compared with NTEG rats, beside reduced exercise tolerance (60±5 vs. 15±3 minutes) (p = 0.002) the 8-OhdG liver tissue concentrations were significantly higher (p = 0.040) in the HTEG rats. The HTEG developed more organ tissue damage and cellular fragmentations of liver cells. In both exercise groups TNF-α and IL-6 serum concentrations were enhanced significantly (p<0.001) being highest in the HTEG animals. Serum ALT, AST, LDH, CREA, BUN and CK concentrations were significantly enhance in both exercise groups.
Conclusion: In our exertional heat stroke rat model, we found tissue damage particularly in livers during exercises in hot and humid environment that was related to inflammation, oxidative stress and apoptosis.
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Exercises in hot and humid environment caused liver injury in a rat model.
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Autor/in / Beteiligte Person: | Li, D ; Wang, X ; Liu, B ; Liu, Y ; Zeng, Z ; Lu, L ; Zheng, Z ; Li, B |
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Zeitschrift: | PloS one, Jg. 9 (2014-12-30), Heft 12, S. e111741 |
Veröffentlichung: | San Francisco, CA : Public Library of Science, 2014 |
Medientyp: | academicJournal |
ISSN: | 1932-6203 (electronic) |
DOI: | 10.1371/journal.pone.0111741 |
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