How to translate a bioassay into a screening assay for natural products: general considerations and implementation of antimicrobial screens.

Fallarero, A ; Hanski, L ; et al.

In: Planta medica, Jg. 80 (2014-09-01), Heft 14, S. 1182

academicJournal

Natural product sources have been a valuable provider of molecular diversity in many drug discovery programs and several therapeutically important drugs have been isolated from these. However, the screening of such materials can be very complicated due to the fact that they contain a complex mixture of secondary metabolites, but also the purified natural compounds exert a challenge for bioactivity screening. Success in identifying new therapeutics using in vitro bioassays is largely dependent upon the proper design, validation, and implementation of the screening assay. In this review, we discuss some aspects which are of significant concern when screening natural products in a microtiter plate-based format, being partly applicable to other assay formats as well, such as validation parameters, layouts for assay protocols, and common interferences caused by natural products samples, as well as various troubleshooting strategies. Examples from the field of natural product drug discovery of antibacterial compounds are discussed, and contributions from the realm of academic screenings are highlighted.

(Georg Thieme Verlag KG Stuttgart · New York.)

Titel:	How to translate a bioassay into a screening assay for natural products: general considerations and implementation of antimicrobial screens.
Autor/in / Beteiligte Person:	Fallarero, A ; Hanski, L ; Vuorela, P
Zeitschrift:	Planta medica, Jg. 80 (2014-09-01), Heft 14, S. 1182
Veröffentlichung:	Stuttgart ; New York : George Thieme ; <i>Original Publication</i>: Stuttgart, Hippokrates Verlag., 2014
Medientyp:	academicJournal
ISSN:	1439-0221 (electronic)
DOI:	10.1055/s-0034-1383061
Schlagwort:	Animals Anti-Bacterial Agents isolation & purification Biological Products chemistry Humans Plant Extracts chemistry Plant Extracts pharmacology Small Molecule Libraries Anti-Bacterial Agents pharmacology Bacteria drug effects Biological Assay methods Biological Products pharmacology Drug Discovery methods
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Review Language: English [Planta Med] 2014 Sep; Vol. 80 (14), pp. 1182-99. <i>Date of Electronic Publication: </i>2014 Sep 15. MeSH Terms: Anti-Bacterial Agents / pharmacology ; Bacteria / drug effects ; Biological Assay / methods ; Biological Products / pharmacology ; Drug Discovery / *methods ; Animals ; Anti-Bacterial Agents / isolation & purification ; Biological Products / chemistry ; Humans ; Plant Extracts / chemistry ; Plant Extracts / pharmacology ; Small Molecule Libraries Substance Nomenclature: 0 (Anti-Bacterial Agents) ; 0 (Biological Products) ; 0 (Plant Extracts) ; 0 (Small Molecule Libraries) Entry Date(s): Date Created: 20140916 Date Completed: 20151021 Latest Revision: 20220311 Update Code: 20231215

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