Icariin ameliorates cigarette smoke induced inflammatory responses via suppression of NF-κB and modulation of GR in vivo and in vitro.
In: PloS one, Jg. 9 (2014-08-04), Heft 8, S. e102345
Online
academicJournal
Zugriff:
Purpose: To investigate the effects of icariin, a major constituent of flavonoids isolated from the herb Epimedium, on cigarette smoke (CS) induced inflammatory responses in vivo and in vitro.
Methods: In vivo, BALB/c mice were exposed to smoke of 15 cigarettes for 1 h/day, 6 days/week for 3 months and dosed with icariin (25, 50 and 100 mg/kg) or dexamethasone (1 mg/kg). In vitro, A549 cells were incubated with icariin (10, 50 and 100 µM) followed by treatments with CSE (2.5%).
Results: We found that icariin significantly protected pulmonary function and attenuated CS-induced inflammatory response by decreasing inflammatory cells and production of TNF-α, IL-8 and MMP-9 in both the serum and BALF of CS-exposed mice and decreasing production of TNF-α and IL-8 in the supernatant of CSE-exposed A549 cells. Icariin also showed properties in inhibiting the phosphorylation of NF-κB p65 protein and blocking the degradation of IΚB-α protein. Further studies revealed that icariin administration markedly restore CS-reduced GR protein and mRNA expression, which might subsequently contribute to the attenuation of CS-induced respiratory inflammatory response.
Conclusion: Together these results suggest that icariin has anti-inflammatory effects in cigarette smoke induced inflammatory models in vivo and in vitro, possibly achieved by suppressing NF-κB activation and modulating GR protein expression.
Titel: |
Icariin ameliorates cigarette smoke induced inflammatory responses via suppression of NF-κB and modulation of GR in vivo and in vitro.
|
---|---|
Autor/in / Beteiligte Person: | Li, L ; Sun, J ; Xu, C ; Zhang, H ; Wu, J ; Liu, B ; Dong, J |
Link: | |
Zeitschrift: | PloS one, Jg. 9 (2014-08-04), Heft 8, S. e102345 |
Veröffentlichung: | San Francisco, CA : Public Library of Science, 2014 |
Medientyp: | academicJournal |
ISSN: | 1932-6203 (electronic) |
DOI: | 10.1371/journal.pone.0102345 |
Schlagwort: |
|
Sonstiges: |
|