[Study on different factors affecting the bionic enzymatic hydrolysis of icariin].
In: Yao xue xue bao = Acta pharmaceutica Sinica, Jg. 48 (2013-11-01), Heft 11, S. 1716
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Zugriff:
This study aims to observe different factors which affected the bionic enzymatic hydrolysis of icariin into baohuoside I and to optimize the reaction conditions in order to provide research foundation for building a novel bionic enzymolysis drug delivery system. To simulate the environment in vivo, 37 degrees C was set as the temperature and artificial intestinal juice and gastric juice were selected as the buffer solutions. Taking the conversion of baohuoside I as index, the effects of the kinds of enzyme, enzyme activity, substrate concentration, reaction time, pancreatin in artificial intestinal juice and surfactant on the conversion of baohuoside I were investigated. The results showed that cellulase, beta-glucosidase and snailase were all inactive in artificial gastric juice and no baohuoside I generated. Pancreatin in artificial intestinal juice couldn't significantly influence the activity of beta-glucosidase or snailase (P > 0.05), but noticeably decrease the activity of cellulase (P < 0.05). In artificial intestinal juice, the conversion of baohuoside I was highest by using beta-glucosidase, and the optimum reaction conditions were determined as follows: enzyme activity 10 U x mL(-1), substrate concentration 1 mg x mL(-1), 3 g x L(-1) rhamnolipid and reaction time 3 h. Under this condition, the conversion of baohuoside I was 99.8%.
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[Study on different factors affecting the bionic enzymatic hydrolysis of icariin].
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Autor/in / Beteiligte Person: | Gao, X ; Chen, Y ; Wang, Y ; Sun, WJ ; Jia, XB |
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Zeitschrift: | Yao xue xue bao = Acta pharmaceutica Sinica, Jg. 48 (2013-11-01), Heft 11, S. 1716 |
Veröffentlichung: | Beijing : Zhongguo yao xue hui, 2013 |
Medientyp: | academicJournal |
ISSN: | 0513-4870 (print) |
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