[Comparison between icariin and genistein in osteogenic activity of marrow stromal cells].
In: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, Jg. 38 (2013-06-01), Heft 11, S. 1783
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Zugriff:
Objective: To compare the effect of icariin and genistein in the osteogenic differentiation of rat bone marrow stromal cells (rBMSC).
Method: Rat marrow stromal cells were seperated in vitro, and the optimal concentration of genisten and icriin were screened. Genistein and icariin with the concentration of 1 x 10(-5) mol x L(-1) were adopted to intereven rBMSCs cultured in vitro. Alkaline phosphatase (ALP) was determined at 3, 6, 9, 12,15 d after intervention; calcified nodule was detected with alizarin red staining at 12 d; OXS, Runx-2, bone morphogenetic protein (BMP-2) and Collagen-I mRNA expression were observed with Real-time RT-PCR at 12, 24, 48, 72, 96 h.
Result: Genistein and icariin with the concentration of 1 x 10(-5) mol x L(-1) could increase the activity of ALP and the content of Ca, regulate OXS, BMP-2, Runx-2 and Collagen-I mRNA expression.
Conclusion: Icariin showed a stronger effect in improving the osteogenic differentiation of rat bone marrow stromal cells than genistein.
| Titel: |
[Comparison between icariin and genistein in osteogenic activity of marrow stromal cells].
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| Autor/in / Beteiligte Person: | Zhou, J ; Chen, KM ; Ge, BF ; Ma, XN ; Guo, XY ; Cheng, K ; Gao, YH ; Yan, LJ ; Shi, WG |
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| Zeitschrift: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, Jg. 38 (2013-06-01), Heft 11, S. 1783 |
| Veröffentlichung: | Beijing : Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo ; <i>Original Publication</i>: [Beijing] : Zhongguo yao xue hui : [Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo, 1989-, 2013 |
| Medientyp: | academicJournal |
| ISSN: | 1001-5302 (print) |
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