[Mechanisms of icariin in regulating bone formation of osteoblasts and bone resorption of osteoclasts].
In: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae, Jg. 35 (2013-08-01), Heft 4, S. 432
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Zugriff:
Objective: To investigate the molecular mechanisms of icariin (ICA) in regulating the bone formation of osteoblasts and the bone resorption of osteoclasts.
Methods: Primary osteoblast cell cultures were obtained from newborn rat calvarial. Calcified nodules were stained by alizarin red. The mRNA levels of osterix (OSX), runt-related transcription factor 2 (Runx-2), alkaline phosphatase (ALP), Collagen1, osteoprotegerin (OPG), and receptor activator of nuclear factor-ΚB ligand (RANKL) were analyzed by quantitative real-time RT-PCR, the protein levels of OPG, RANKL, and Collagen1 were examined by Western blotting, and the intracellular Ca(2+) concentration of osteoblasts was measured on a flow cytometer using the Cellquest program.
Results: Compared with control group, ICA markedly promoted bone formation by significant up-regulating the gene expressions of OSX, Runx-2,ALP, and Collagen1, the protein expression of Collagen1(all P<0.01), and the Ca(2+) concentration. Furthermore, ICA remarkably inhibited bone resorption by significant up-regulating the mRNA and protein expressions of OPG as well as the OPG/RANKL ratio.
Conclusions: ICA could promote bone formation of osteoblasts through inducting the gene expressions of OSX,Runx-2, ALP and Collagen1, and the protein expressions of Collagen1, and by increasing the Ca (2+) concentration. Moreover, ICA could inhibit bone resorption of osteoclasts through regulating OPG/RANKL signal pathway.
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[Mechanisms of icariin in regulating bone formation of osteoblasts and bone resorption of osteoclasts].
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Autor/in / Beteiligte Person: | Ma, XN ; Ge, BF ; Chen, KM ; Zhou, J ; Shi, WG ; Xie, YF ; Guo, XY ; Lv, X ; Cheng, K ; Gao, YH |
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Zeitschrift: | Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae, Jg. 35 (2013-08-01), Heft 4, S. 432 |
Veröffentlichung: | Beijing : Zhongguo yi xue ke xue yuan ; <i>Original Publication</i>: [Beijing] Zhongguo yi xue ke xue yuan., 2013 |
Medientyp: | academicJournal |
ISSN: | 1000-503X (print) |
DOI: | 10.3881/j.issn.1000-503X.2013.04.014 |
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