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Icariin, the main active compound of the traditional Chinese medicine, Epimedium, is commonly used for the clinical treatment of osteoporosis. However, the precise molecular mechanism of the therapeutic effect of icariin has not been elucidated. The aim of this study was to examine the effect of icariin on cell viability, alkaline phosphatase (ALP) activity, the amount of calcified nodules, and to delineate the molecular mechanism of icariin-enhanced bone formation by investigating the expression of bone morphogenic protein-2 (BMP-2), Smad4, Cbfa1/Runx2, osteoprotegerin (OPG), receptor activator of nuclear factor κ-B ligand (RANKL) and the OPG/RANKL ratio in the hFOB 1.19 human osteoblastic cell line. We found that icariin significantly increased the cell viability, the activity of ALP and the amount of calcified nodules in the hFOB 1.19 cells. Furthermore, we observed that icariin upregulated the expression of BMP-2, Smad4, Cbfa1/Runx2, OPG, RANKL and the OPG/RANKL ratio. Our results indicate that icariin can modulate the process of bone formation via the BMP-2/Smad4 signal transduction pathway in hFOB 1.19 cells.

Titel:	Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line.
Autor/in / Beteiligte Person:	Liang, W ; Lin, M ; Li, X ; Li, C ; Gao, B ; Gan, H ; Yang, Z ; Lin, X ; Liao, L ; Yang, M
Zeitschrift:	International journal of molecular medicine, Jg. 30 (2012-10-01), Heft 4, S. 889
Veröffentlichung:	Athens, Greece : D.A. Spandidos, [1998-, 2012
Medientyp:	academicJournal
ISSN:	1791-244X (electronic)
DOI:	10.3892/ijmm.2012.1079
Schlagwort:	Alkaline Phosphatase metabolism Bone Morphogenetic Protein 2 genetics Cell Line Cell Survival drug effects Core Binding Factor Alpha 1 Subunit genetics Core Binding Factor Alpha 1 Subunit metabolism Drugs, Chinese Herbal chemistry Epimedium chemistry Flavonoids chemistry Gene Expression Regulation, Developmental drug effects Humans Osteoblasts metabolism Osteoprotegerin genetics Osteoprotegerin metabolism RANK Ligand genetics RANK Ligand metabolism RNA, Messenger genetics Signal Transduction drug effects Smad4 Protein genetics Bone Morphogenetic Protein 2 metabolism Drugs, Chinese Herbal pharmacology Flavonoids pharmacology Osteoblasts drug effects Osteogenesis drug effects Smad4 Protein metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Int J Mol Med] 2012 Oct; Vol. 30 (4), pp. 889-95. <i>Date of Electronic Publication: </i>2012 Jul 26. MeSH Terms: Bone Morphogenetic Protein 2 / metabolism ; Drugs, Chinese Herbal / pharmacology ; Flavonoids / pharmacology ; Osteoblasts / drug effects ; Osteogenesis / drug effects ; Smad4 Protein / metabolism ; Alkaline Phosphatase / metabolism ; Bone Morphogenetic Protein 2 / genetics ; Cell Line ; Cell Survival / drug effects ; Core Binding Factor Alpha 1 Subunit / genetics ; Core Binding Factor Alpha 1 Subunit / metabolism ; Drugs, Chinese Herbal / chemistry ; Epimedium / chemistry ; Flavonoids / chemistry ; Gene Expression Regulation, Developmental / drug effects ; Humans ; Osteoblasts / metabolism ; Osteoprotegerin / genetics ; Osteoprotegerin / metabolism ; RANK Ligand / genetics ; RANK Ligand / metabolism ; RNA, Messenger / genetics ; Signal Transduction / drug effects ; Smad4 Protein / genetics Substance Nomenclature: 0 (BMP2 protein, human) ; 0 (Bone Morphogenetic Protein 2) ; 0 (Core Binding Factor Alpha 1 Subunit) ; 0 (Drugs, Chinese Herbal) ; 0 (Flavonoids) ; 0 (Osteoprotegerin) ; 0 (RANK Ligand) ; 0 (RNA, Messenger) ; 0 (RUNX2 protein, human) ; 0 (Smad4 Protein) ; 0 (TNFSF11 protein, human) ; EC 3.1.3.1 (Alkaline Phosphatase) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20120731 Date Completed: 20130524 Latest Revision: 20220318 Update Code: 20240513

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Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line.