High-performance liquid chromatography-based multivariate analysis to predict the estrogenic activity of an Epimedium koreanum extract.
In: Bioscience, biotechnology, and biochemistry, Jg. 76 (2012), Heft 5, S. 923
Online
academicJournal
Zugriff:
This study characterizes the correlation between the chemical fingerprint and estrogenic activity of an Epimedium koreanum extract. The estrogenic activity of 31 E. koreanum extract samples was evaluated by a luciferase reporter gene assay, and the samples were classified into 3 groups based on their bioactivity. A chemical fingerprint analysis was performed on each sample by high-performance liquid chromatography (HPLC), and 44 common peaks were selected from the chromatogram and used as a dataset for a pattern recognition analysis. A canonical discriminant analysis performed on this dataset determined a distinct distribution of the samples according to their estrogenic activity on the scoring plot. The classification results showed that 90.3% of the original grouped cases had been correctly classified. The total content of the 4 major extract compounds, epimedin A, epimedin B, epimedin C, and icariin, exhibited good correlation (r=0.784) with the estrogenic activities of the respective extracts. This chromatographic fingerprint-chemometric analysis system could be useful for predicting the E. koreanum pharmacological activity and consequent biological activity-relevant quality control assessment.
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High-performance liquid chromatography-based multivariate analysis to predict the estrogenic activity of an Epimedium koreanum extract.
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Autor/in / Beteiligte Person: | Islam, MN ; Kim, U ; Kim, DH ; Dong, MS ; Yoo, HH |
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Zeitschrift: | Bioscience, biotechnology, and biochemistry, Jg. 76 (2012), Heft 5, S. 923 |
Veröffentlichung: | 2021- : Oxford : Oxford University Press ; <i>Original Publication</i>: Tokyo, Japan : Japan Society for Bioscience, Biotechnology, and Agrochemistry, c1992-, 2012 |
Medientyp: | academicJournal |
ISSN: | 1347-6947 (electronic) |
DOI: | 10.1271/bbb.110922 |
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