Attenuation of LPS-induced inflammation by ICT, a derivate of icariin, via inhibition of the CD14/TLR4 signaling pathway in human monocytes.
In: International immunopharmacology, Jg. 12 (2012), Heft 1, S. 74
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Zugriff:
Objective: To evaluate the anti-inflammatory potential of ICT in LPS stimulated human innate immune cells.
Background: 3, 5, 7-Trihydroxy-4'-methoxy-8-(3-hydroxy-3- methylbutyl)-flavone (ICT) is a novel derivative of icariin, the major active ingredient of Herba Epimedii, an herb used in traditional Chinese medicine. We previously demonstrated its anti-inflammatory potential in a murine macrophage cell line as well as in mouse models.
Methods: We measured TNF-α production by ELISA, TLR4/CD14 expression by flow cytometry, and NF-κB and MAPK activation by western blot all in LPS-stimulated PBMC, human monocytes, or THP-1 cells after treatment with ICT.
Results: ICT inhibited LPS-induced TNF-α production in THP-1 cells, PBMCs and human monocytes in a dose-dependent manner. ICT treatment resulted in down-regulation of the expression of CD14/TLR4 and attenuated NF-κB and MAPK activation induced by LPS.
Conclusion: We illustrate the anti-inflammatory property of ICT in human immune cells, especially in monocytes. These effects were mediated, at least partially, via inhibition of the CD14/TLR4 signaling pathway.
(Copyright © 2011 Elsevier B.V. All rights reserved.)
| Titel: |
Attenuation of LPS-induced inflammation by ICT, a derivate of icariin, via inhibition of the CD14/TLR4 signaling pathway in human monocytes.
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| Autor/in / Beteiligte Person: | Wu, J ; Zhou, J ; Chen, X ; Fortenbery, N ; Eksioglu, EA ; Wei, S ; Dong, J |
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| Zeitschrift: | International immunopharmacology, Jg. 12 (2012), Heft 1, S. 74 |
| Veröffentlichung: | Amsterdam ; New York : Elsevier Science, c2001-, 2012 |
| Medientyp: | academicJournal |
| ISSN: | 1878-1705 (electronic) |
| DOI: | 10.1016/j.intimp.2011.10.015 |
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