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Small nuclear and nucleolar RNAs that program pre-mRNA splicing and rRNA processing have a signature 5'-trimethylguanosine (TMG) cap. Whereas the mechanism of TMG synthesis by Tgs1 methyltransferase has been elucidated, we know little about whether or how RNP biogenesis, structure and function are perturbed when TMG caps are missing. Here, we analyzed RNPs isolated by tandem-affinity purification from TGS1 and tgs1Δ yeast strains. The protein and U-RNA contents of total SmB-containing RNPs were similar. Finer analysis revealed stoichiometric association of the nuclear cap-binding protein (CBP) subunits Sto1 and Cbc2 with otherwise intact Mud1- and Nam8-containing U1 snRNPs from tgs1Δ cells. CBP was not comparably enriched in Lea1-containing U2 snRNPs from tgs1Δ cells. Moreover, CBP was not associated with mature Nop58-containing C/D snoRNPs or mature Cbf5- and Gar1-containing H/ACA snoRNPs from tgs1Δ cells. The protein composition and association of C/D snoRNPs with the small subunit (SSU) processosome were not grossly affected by absence of TMG caps, nor was the composition of H/ACA snoRNPs. The cold-sensitive (cs) growth defect of tgs1Δ yeast cells could be suppressed by mutating the cap-binding pocket of Cbc2, suggesting that ectopic CBP binding to the exposed U1 m(7)G cap in tgs1Δ cells (not lack of TMG caps per se) underlies the cs phenotype.

Titel:	Composition of yeast snRNPs and snoRNPs in the absence of trimethylguanosine caps reveals nuclear cap binding protein as a gained U1 component implicated in the cold-sensitivity of tgs1Δ cells.
Autor/in / Beteiligte Person:	Schwer, B ; Erdjument-Bromage, H ; Shuman, S
Link:	Zum Volltext
Zeitschrift:	Nucleic acids research, Jg. 39 (2011-08-01), Heft 15, S. 6715-28
Veröffentlichung:	1992- : Oxford : Oxford University Press ; <i>Original Publication</i>: London, Information Retrieval ltd., 2011
Medientyp:	academicJournal
ISSN:	1362-4962 (electronic)
DOI:	10.1093/nar/gkr279
Schlagwort:	Autoantigens isolation & purification Cold Temperature Gene Deletion Nuclear Cap-Binding Protein Complex chemistry Nuclear Cap-Binding Protein Complex genetics Nuclear Cap-Binding Protein Complex isolation & purification Phenotype RNA Caps metabolism Ribonucleoprotein, U1 Small Nuclear chemistry Ribonucleoprotein, U1 Small Nuclear isolation & purification Ribonucleoprotein, U2 Small Nuclear chemistry Ribonucleoprotein, U2 Small Nuclear isolation & purification Ribonucleoproteins, Small Nuclear isolation & purification Ribonucleoproteins, Small Nucleolar isolation & purification Saccharomyces cerevisiae growth & development Saccharomyces cerevisiae Proteins chemistry Saccharomyces cerevisiae Proteins genetics Saccharomyces cerevisiae Proteins isolation & purification Suppression, Genetic Methyltransferases genetics Nuclear Cap-Binding Protein Complex analysis Ribonucleoproteins, Small Nuclear chemistry Ribonucleoproteins, Small Nucleolar chemistry Saccharomyces cerevisiae genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, N.I.H., Extramural Language: English [Nucleic Acids Res] 2011 Aug; Vol. 39 (15), pp. 6715-28. <i>Date of Electronic Publication: </i>2011 May 10. MeSH Terms: Methyltransferases / genetics ; Nuclear Cap-Binding Protein Complex / analysis ; Ribonucleoproteins, Small Nuclear / chemistry ; Ribonucleoproteins, Small Nucleolar / chemistry ; Saccharomyces cerevisiae / *genetics ; Autoantigens / isolation & purification ; Cold Temperature ; Gene Deletion ; Nuclear Cap-Binding Protein Complex / chemistry ; Nuclear Cap-Binding Protein Complex / genetics ; Nuclear Cap-Binding Protein Complex / isolation & purification ; Phenotype ; RNA Caps / metabolism ; Ribonucleoprotein, U1 Small Nuclear / chemistry ; Ribonucleoprotein, U1 Small Nuclear / isolation & purification ; Ribonucleoprotein, U2 Small Nuclear / chemistry ; Ribonucleoprotein, U2 Small Nuclear / isolation & purification ; Ribonucleoproteins, Small Nuclear / isolation & purification ; Ribonucleoproteins, Small Nucleolar / isolation & purification ; Saccharomyces cerevisiae / growth & development ; Saccharomyces cerevisiae Proteins / chemistry ; Saccharomyces cerevisiae Proteins / genetics ; Saccharomyces cerevisiae Proteins / isolation & purification ; Suppression, Genetic References: EMBO J. 1995 Sep 1;14(17):4350-6. (PMID: 7556076) ; J Biol Chem. 2005 Feb 11;280(6):4021-4. (PMID: 15590684) ; Genes Dev. 1996 Jul 1;10(13):1699-708. (PMID: 8682299) ; RNA. 2001 Nov;7(11):1554-65. (PMID: 11720285) ; Mol Cell Biol. 1999 Oct;19(10):6543-53. (PMID: 10490594) ; EMBO J. 1995 Aug 15;14(16):4001-9. (PMID: 7664739) ; EMBO J. 2010 Dec 15;29(24):4172-84. (PMID: 21113136) ; Methods. 2002 Mar;26(3):260-9. (PMID: 12054882) ; J Biol Chem. 2005 Sep 16;280(37):32101-6. (PMID: 16046409) ; EMBO J. 1996 Aug 15;15(16):4368-79. (PMID: 8861964) ; Mol Cell Biol. 2009 Jul;29(14):3941-52. (PMID: 19451230) ; Proc Natl Acad Sci U S A. 1997 Jan 21;94(2):385-90. (PMID: 9012791) ; EMBO J. 1999 Jun 15;18(12):3463-74. (PMID: 10369685) ; RNA. 2010 May;16(5):1018-31. (PMID: 20360394) ; Nat Biotechnol. 1999 Oct;17(10):1030-2. (PMID: 10504710) ; Mol Cell Biol. 1999 Jan;19(1):577-84. (PMID: 9858581) ; Eukaryot Cell. 2004 Dec;3(6):1619-26. (PMID: 15590835) ; J Biol Chem. 2008 Nov 14;283(46):31706-18. (PMID: 18775984) ; Nat Struct Biol. 2002 Dec;9(12):912-7. (PMID: 12434151) ; EMBO J. 1999 Aug 16;18(16):4535-48. (PMID: 10449419) ; RNA. 1998 Apr;4(4):374-93. (PMID: 9630245) ; Nature. 2009 Mar 26;458(7237):475-80. (PMID: 19325628) ; Nucleic Acids Res. 2011 Apr;39(8):3427-45. (PMID: 21208980) ; Mol Cell. 2010 Mar 12;37(5):597-606. (PMID: 20227365) ; Mol Cell. 2005 Jul 1;19(1):53-63. (PMID: 15989964) ; Genes Dev. 1999 Sep 15;13(18):2425-38. (PMID: 10500099) ; Genes Dev. 1996 Jul 1;10(13):1683-98. (PMID: 8682298) ; Cell. 1996 Oct 4;87(1):21-32. (PMID: 8858145) ; EMBO J. 2002 Oct 15;21(20):5548-57. (PMID: 12374755) ; Mol Cell. 2002 Apr;9(4):891-901. (PMID: 11983179) ; RNA. 1998 Dec;4(12):1549-68. (PMID: 9848653) ; RNA Biol. 2009 Nov-Dec;6(5):563-74. (PMID: 19838078) ; RNA. 2006 Jun;12(6):968-79. (PMID: 16618970) ; J Biol Chem. 2000 Aug 4;275(31):23718-24. (PMID: 10823828) ; RNA. 2010 Jan;16(1):211-20. (PMID: 19926722) ; Nature. 2006 Mar 30;440(7084):637-43. (PMID: 16554755) ; J Mol Biol. 2009 Jan 16;385(2):618-27. (PMID: 19026660) ; Genes Dev. 1993 Mar;7(3):419-28. (PMID: 8449403) ; Mol Cell Biol. 1998 Dec;18(12):7383-96. (PMID: 9819425) ; RNA Biol. 2009 Jan-Mar;6(1):17-20. (PMID: 19106629) ; Nucleic Acids Res. 2007;35(5):1411-20. (PMID: 17284461) ; J Mol Biol. 2001 Apr 20;308(1):49-58. (PMID: 11302706) ; Nature. 2006 Mar 30;440(7084):631-6. (PMID: 16429126) ; Nature. 2002 Jun 27;417(6892):967-70. (PMID: 12068309) ; Mol Cell. 2009 Nov 25;36(4):593-608. (PMID: 19941820) ; Mol Cell. 2008 Dec 5;32(5):735-46. (PMID: 19061648) ; J Chromatogr A. 1998 Nov 27;826(2):167-81. (PMID: 9871337) ; Mol Cell. 2002 Jan;9(1):31-44. (PMID: 11804584) ; Nucleic Acids Res. 2011 Mar;39(6):2188-209. (PMID: 21075799) ; Nat Struct Mol Biol. 2009 Sep;16(9):930-7. (PMID: 19668212) ; Nat Methods. 2007 Nov;4(11):951-6. (PMID: 17922018) Grant Information: R01 GM052470 United States GM NIGMS NIH HHS; P30 CA08748 United States CA NCI NIH HHS; GM52470 United States GM NIGMS NIH HHS; GM50288 United States GM NIGMS NIH HHS; R01 GM050288 United States GM NIGMS NIH HHS Substance Nomenclature: 0 (Autoantigens) ; 0 (CBC2 protein, S cerevisiae) ; 0 (LEA1 protein, S cerevisiae) ; 0 (Nuclear Cap-Binding Protein Complex) ; 0 (RNA Caps) ; 0 (Ribonucleoprotein, U1 Small Nuclear) ; 0 (Ribonucleoprotein, U2 Small Nuclear) ; 0 (Ribonucleoproteins, Small Nuclear) ; 0 (Ribonucleoproteins, Small Nucleolar) ; 0 (SMB protein, S cerevisiae) ; 0 (Saccharomyces cerevisiae Proteins) ; EC 2.1.1.- (Methyltransferases) ; EC 2.1.1.- (trimethylguanosine synthase) Entry Date(s): Date Created: 20110512 Date Completed: 20111101 Latest Revision: 20211020 Update Code: 20231215 PubMed Central ID: PMC3159458

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Composition of yeast snRNPs and snoRNPs in the absence of trimethylguanosine caps reveals nuclear cap binding protein as a gained U1 component implicated in the cold-sensitivity of tgs1Δ cells.