[Comparative study on the osteogenic differentiation of rat bone marrow stromal cells effected by icariin and icariside II].

Zhai, YK ; Ge, BF ; et al.

In: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, Jg. 33 (2010-12-01), Heft 12, S. 1896

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Objective: To investigate the effects of icariin and it's main metabolites-icariside II on the osteogenic differentiation of rat bone marrow stromal cells (rBMSCs).

Methods: rBMSCs were cultured by adherence screening method, icariin and icariside II were supplemented into the culture at 5 x 10(-5) mol/L respectively. The osteogenic differentiation markers including alkaline phosphatase (ALP) activity, CFU-F(ALp), osteocalcin secretion, calcium deposition and mineralized bone modulus were compared among the icariin-supplemented group, icariside II and the control. The gene expressions of bFGF, IGF-1, Osterix and Runx-2 were examined by RT-Real Time PCR.

Results: Both icariside II and icariin significantly improved ALP activity, CFU-F(ALP) amount, osteocalcin secretion, calcium deposition and mineralized modulus. Besides, they enhanced the gene expressions of bFGF, IGF-1, Osterix and Runx-2. Icariside II was obviously stronger than icariin at the above activities.

Conclusion: Icariside II is stronger than icariin at enhancing the osteogenic differentiation of rBMSCs, suggesting that icariin can be administered via oral and it's metabolites are the effective constitutes for antiosteoporosis activity.

Titel:	[Comparative study on the osteogenic differentiation of rat bone marrow stromal cells effected by icariin and icariside II].
Autor/in / Beteiligte Person:	Zhai, YK ; Ge, BF ; Chen, KM ; Ma, HP ; Ming, LG ; Li, ZF
Link:	Zum Volltext
Zeitschrift:	Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, Jg. 33 (2010-12-01), Heft 12, S. 1896
Veröffentlichung:	Guangzhou Shi : Guo jia yi yao guan li ju Zhong yao cai qing bao zhong xin zhan ; <i>Original Publication</i>: Guangzhou Shi : Guo jia yi yao guan li ju Zhong yao cai qing bao zhong xin zhan,, 2010
Medientyp:	academicJournal
ISSN:	1001-4454 (print)
Schlagwort:	Alkaline Phosphatase metabolism Animals Bone Marrow Cells drug effects Bone Marrow Cells metabolism Cells, Cultured Female Fibroblast Growth Factor 2 genetics Fibroblast Growth Factor 2 metabolism Insulin-Like Growth Factor I genetics Insulin-Like Growth Factor I metabolism Osteocalcin metabolism Osteogenesis drug effects RNA, Messenger genetics RNA, Messenger metabolism Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction Stromal Cells drug effects Stromal Cells metabolism Bone Marrow Cells cytology Cell Differentiation drug effects Drugs, Chinese Herbal pharmacology Flavonoids pharmacology Stromal Cells cytology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: Chinese Publication Type: Comparative Study; Journal Article Language: Chinese [Zhong Yao Cai] 2010 Dec; Vol. 33 (12), pp. 1896-900. MeSH Terms: Bone Marrow Cells / cytology ; Cell Differentiation / drug effects ; Drugs, Chinese Herbal / pharmacology ; Flavonoids / pharmacology ; Stromal Cells / *cytology ; Alkaline Phosphatase / metabolism ; Animals ; Bone Marrow Cells / drug effects ; Bone Marrow Cells / metabolism ; Cells, Cultured ; Female ; Fibroblast Growth Factor 2 / genetics ; Fibroblast Growth Factor 2 / metabolism ; Insulin-Like Growth Factor I / genetics ; Insulin-Like Growth Factor I / metabolism ; Osteocalcin / metabolism ; Osteogenesis / drug effects ; RNA, Messenger / genetics ; RNA, Messenger / metabolism ; Rats ; Rats, Wistar ; Reverse Transcriptase Polymerase Chain Reaction ; Stromal Cells / drug effects ; Stromal Cells / metabolism Substance Nomenclature: 0 (Drugs, Chinese Herbal) ; 0 (Flavonoids) ; 0 (RNA, Messenger) ; 103107-01-3 (Fibroblast Growth Factor 2) ; 104982-03-8 (Osteocalcin) ; 113558-15-9 (baohuoside I) ; 67763-96-6 (Insulin-Like Growth Factor I) ; EC 3.1.3.1 (Alkaline Phosphatase) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20110510 Date Completed: 20111128 Latest Revision: 20161125 Update Code: 20240513

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