Characterization of IncF plasmids carrying the blaCTX-M-14 gene in clinical isolates of Escherichia coli from Korea.

Kim, J ; Bae, IK ; et al.

In: The Journal of antimicrobial chemotherapy, Jg. 66 (2011-06-01), Heft 6, S. 1263-8

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Objectives: The purpose of this study was to investigate the molecular epidemiology of CTX-M-14-producing Escherichia coli clinical isolates from Korea.

Methods: A total of 138 non-duplicate E. coli clinical isolates showing reduced susceptibility or resistance to ceftazidime and/or cefotaxime were included in the study. Resistance genes, genetic environment, R plasmid size and replicon type, sequence type (ST) and XbaI-macrorestriction patterns were determined.

Results: Among 138 isolates, 35 were found to carry the bla(CTX-M-14) gene. The ISEcp1 element was identified in the upstream region of the bla(CTX-M-14) gene in 32 isolates. The bla(CTX-M-14) gene was located on an IncF plasmid in 21 isolates, on an IncA/C plasmid in 1 isolate, on the chromosome in 8 isolates and on both the chromosome and an IncF plasmid in 5 isolates. The most prevalent ST was ST405 (n = 8), followed by ST354 (n = 4), ST38 (n = 3), ST69 (n = 3) and the intercontinental ST, ST131 (n = 3). PFGE and multilocus sequence typing experiments demonstrated no major clonal relationship among the CTX-M-14-producing isolates.

Conclusions: The bla(CTX-M-14) gene was probably mobilized by IncF plasmids, which can readily spread in E. coli, causing horizontal dissemination of the resistance gene in Korea.

Titel:	Characterization of IncF plasmids carrying the blaCTX-M-14 gene in clinical isolates of Escherichia coli from Korea.
Autor/in / Beteiligte Person:	Kim, J ; Bae, IK ; Jeong, SH ; Chang, CL ; Lee, CH ; Lee, K
Link:	Zum Volltext
Zeitschrift:	The Journal of antimicrobial chemotherapy, Jg. 66 (2011-06-01), Heft 6, S. 1263-8
Veröffentlichung:	1997- : London : Oxford University Press ; <i>Original Publication</i>: London, New York, Academic Press., 2011
Medientyp:	academicJournal
ISSN:	1460-2091 (electronic)
DOI:	10.1093/jac/dkr106
Schlagwort:	Anti-Bacterial Agents pharmacology Bacterial Typing Techniques Cefotaxime pharmacology Ceftazidime pharmacology DNA, Bacterial chemistry DNA, Bacterial genetics DNA, Bacterial metabolism Deoxyribonucleases, Type II Site-Specific metabolism Escherichia coli classification Escherichia coli genetics Gene Transfer, Horizontal Korea epidemiology Molecular Epidemiology Molecular Sequence Data Molecular Typing Polymorphism, Restriction Fragment Length Sequence Analysis, DNA beta-Lactam Resistance Escherichia coli enzymology Escherichia coli isolation & purification Escherichia coli Infections epidemiology Escherichia coli Infections microbiology Plasmids beta-Lactamases genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [J Antimicrob Chemother] 2011 Jun; Vol. 66 (6), pp. 1263-8. <i>Date of Electronic Publication: </i>2011 Mar 17. MeSH Terms: Plasmids* ; Escherichia coli / enzymology ; Escherichia coli / isolation & purification ; Escherichia coli Infections / epidemiology ; Escherichia coli Infections / microbiology ; beta-Lactamases / *genetics ; Anti-Bacterial Agents / pharmacology ; Bacterial Typing Techniques ; Cefotaxime / pharmacology ; Ceftazidime / pharmacology ; DNA, Bacterial / chemistry ; DNA, Bacterial / genetics ; DNA, Bacterial / metabolism ; Deoxyribonucleases, Type II Site-Specific / metabolism ; Escherichia coli / classification ; Escherichia coli / genetics ; Gene Transfer, Horizontal ; Korea / epidemiology ; Molecular Epidemiology ; Molecular Sequence Data ; Molecular Typing ; Polymorphism, Restriction Fragment Length ; Sequence Analysis, DNA ; beta-Lactam Resistance Molecular Sequence: GENBANK EU136399 Substance Nomenclature: 0 (Anti-Bacterial Agents) ; 0 (DNA, Bacterial) ; 9M416Z9QNR (Ceftazidime) ; EC 3.1.21.- (endodeoxyribonuclease XBAI) ; EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific) ; EC 3.5.2.- (beta-lactamase CTX-M-14) ; EC 3.5.2.6 (beta-Lactamases) ; N2GI8B1GK7 (Cefotaxime) Entry Date(s): Date Created: 20110319 Date Completed: 20110823 Latest Revision: 20220321 Update Code: 20231215

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