A molecular probe for Basidiomycota: the spermidine synthase-saccharopine dehydrogenase chimeric gene.

León-Ramírez, CG ; Valdés-Santiago, L ; et al.

In: FEMS microbiology letters, Jg. 312 (2010-11-01), Heft 1, S. 77-83

Online academicJournal

Zugriff:

Zum Volltext

By means of an in silico analysis, we demonstrated that a previously described chimeric gene (Spe-Sdh) encoding spermidine synthase, a key enzyme involved in the synthesis of polyamines, and saccharopine dehydrogenase, an enzyme involved in lysine synthesis in fungi, were present exclusively in members of all Basidiomycota subphyla, but not in any other group of living organisms. We used this feature to design degenerated primers to amplify a specific fragment of the Spe-Sdh gene by PCR, as a tool to unequivocally identify Basidiomycota isolates. The specificity of this procedure was tested using different fungal species. As expected, positive results were obtained only with Basidiomycota species, whereas no amplification was achieved with species belonging to other fungal phyla.

Titel:	A molecular probe for Basidiomycota: the spermidine synthase-saccharopine dehydrogenase chimeric gene.
Autor/in / Beteiligte Person:	León-Ramírez, CG ; Valdés-Santiago, L ; Campos-Góngora, E ; Ortiz-Castellanos, L ; Aréchiga-Carvajal, ET ; Ruiz-Herrera, J
Link:	Zum Volltext
Zeitschrift:	FEMS microbiology letters, Jg. 312 (2010-11-01), Heft 1, S. 77-83
Veröffentlichung:	2015- : Oxford Oxford University Press ; <i>Original Publication</i>: Amsterdam, Published by Elsevier/North Holland on behalf of the Federation of European Microbiological Societies., 2010
Medientyp:	academicJournal
ISSN:	1574-6968 (electronic)
DOI:	10.1111/j.1574-6968.2010.02099.x
Schlagwort:	Amino Acid Sequence Base Sequence Basidiomycota chemistry Basidiomycota classification Basidiomycota genetics DNA Primers genetics Fungal Proteins chemistry Fungal Proteins metabolism Molecular Sequence Data Phylogeny Polymerase Chain Reaction Saccharopine Dehydrogenases chemistry Saccharopine Dehydrogenases metabolism Sequence Alignment Species Specificity Spermidine Synthase chemistry Spermidine Synthase metabolism Basidiomycota enzymology Fungal Proteins genetics Molecular Probes genetics Saccharopine Dehydrogenases genetics Spermidine Synthase genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [FEMS Microbiol Lett] 2010 Nov; Vol. 312 (1), pp. 77-83. <i>Date of Electronic Publication: </i>2010 Sep 15. MeSH Terms: Basidiomycota / enzymology ; Fungal Proteins / genetics ; Molecular Probes / genetics ; Saccharopine Dehydrogenases / genetics ; Spermidine Synthase / *genetics ; Amino Acid Sequence ; Base Sequence ; Basidiomycota / chemistry ; Basidiomycota / classification ; Basidiomycota / genetics ; DNA Primers / genetics ; Fungal Proteins / chemistry ; Fungal Proteins / metabolism ; Molecular Sequence Data ; Phylogeny ; Polymerase Chain Reaction ; Saccharopine Dehydrogenases / chemistry ; Saccharopine Dehydrogenases / metabolism ; Sequence Alignment ; Species Specificity ; Spermidine Synthase / chemistry ; Spermidine Synthase / metabolism Substance Nomenclature: 0 (DNA Primers) ; 0 (Fungal Proteins) ; 0 (Molecular Probes) ; EC 1.5.1.- (Saccharopine Dehydrogenases) ; EC 2.5.1.16 (Spermidine Synthase) Entry Date(s): Date Created: 20100918 Date Completed: 20110125 Latest Revision: 20101102 Update Code: 20240513

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.