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The aim of the present work was to explore the anti-hepatoma effects of icariin both in vitro and in vivo and to elucidate its potential mechanism of action. The MTT assay was applied to test the anti-proliferative effects of icariin in vitro. HepG2 bearing NMRI nu/nu mice were used to test the anticancer effects of icariin in vivo. Immunohistochemical assay and flow cytometry assay (FACS) were applied to detect the possible mechanisms of action of icariin. MTT assay illustrated that icariin inhibited the proliferation of HepG2 cells in a concentration dependent manner; meanwhile, icariin inhibited the tumor growth in HepG2 bearing NMRI nu/nu mice. The tumor weight was inhibited by 55.6% and tumor volume was inhibited by 47.2%. Icariin did not influence the spleen and body weights or blood parameters. Immunohistochemical analysis indicated that the expressions of both CD31 and Ki67 in the icariin treated group were significantly lower than those in the control group (p < 0.01). FACS assay showed that icariin dramatically decreased the percentage of CD4+ and CD8+ cells in bone marrow and CD19+ cells in blood on day 8. On day 17, the percentage of CD8+ cells in blood was lower than those in the control group. CD4/CD8 ratio in icariin group was significantly elevated in bone marrow on day 17. Icariin showed anticancer efficacy both in vitro and in vivo. The possible mechanism of action could be related to its anti-angiogenesis and anti-proliferative effects in tumors.

Titel:	Anti-proliferative efficacy of icariin on HepG2 hepatoma and its possible mechanism of action.
Autor/in / Beteiligte Person:	Yang, JX ; Fichtner, I ; Becker, M ; Lemm, M ; Wang, XM
Zeitschrift:	The American journal of Chinese medicine, Jg. 37 (2009), Heft 6, S. 1153
Veröffentlichung:	<2003->: Singapore ; River Edge, NJ : World Scientific Pub. ; <i>Original Publication</i>: [Garden City, N.Y.] : Institute for Advanced Research in Asian Science and Medicine, 2009
Medientyp:	academicJournal
ISSN:	1793-6853 (electronic)
DOI:	10.1142/S0192415X09007569
Schlagwort:	Animals Antigens, CD19 blood Antineoplastic Agents, Phytogenic therapeutic use Bone Marrow immunology CD4-CD8 Ratio CD4-Positive T-Lymphocytes metabolism CD8-Positive T-Lymphocytes metabolism Carcinoma, Hepatocellular metabolism Cell Line, Tumor Dose-Response Relationship, Drug Flavonoids therapeutic use Flow Cytometry Hep G2 Cells Ki-67 Antigen metabolism Liver Neoplasms, Experimental metabolism Male Mice Mice, Nude Phytotherapy Plant Extracts therapeutic use Platelet Endothelial Cell Adhesion Molecule-1 metabolism Xenograft Model Antitumor Assays Antineoplastic Agents, Phytogenic pharmacology Carcinoma, Hepatocellular drug therapy Cell Proliferation drug effects Epimedium chemistry Flavonoids pharmacology Liver Neoplasms, Experimental drug therapy Plant Extracts pharmacology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Am J Chin Med] 2009; Vol. 37 (6), pp. 1153-65. MeSH Terms: Antineoplastic Agents, Phytogenic / pharmacology ; Carcinoma, Hepatocellular / drug therapy ; Cell Proliferation / drug effects ; Epimedium / chemistry ; Flavonoids / pharmacology ; Liver Neoplasms, Experimental / drug therapy ; Plant Extracts / *pharmacology ; Animals ; Antigens, CD19 / blood ; Antineoplastic Agents, Phytogenic / therapeutic use ; Bone Marrow / immunology ; CD4-CD8 Ratio ; CD4-Positive T-Lymphocytes / metabolism ; CD8-Positive T-Lymphocytes / metabolism ; Carcinoma, Hepatocellular / metabolism ; Cell Line, Tumor ; Dose-Response Relationship, Drug ; Flavonoids / therapeutic use ; Flow Cytometry ; Hep G2 Cells ; Ki-67 Antigen / metabolism ; Liver Neoplasms, Experimental / metabolism ; Male ; Mice ; Mice, Nude ; Phytotherapy ; Plant Extracts / therapeutic use ; Platelet Endothelial Cell Adhesion Molecule-1 / metabolism ; Xenograft Model Antitumor Assays Substance Nomenclature: 0 (Antigens, CD19) ; 0 (Antineoplastic Agents, Phytogenic) ; 0 (Flavonoids) ; 0 (Ki-67 Antigen) ; 0 (Plant Extracts) ; 0 (Platelet Endothelial Cell Adhesion Molecule-1) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20091126 Date Completed: 20100408 Latest Revision: 20220318 Update Code: 20231215

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Anti-proliferative efficacy of icariin on HepG2 hepatoma and its possible mechanism of action.