Einzeltreffer — DigiBib

This review is based on the honor of receiving the Discovery Award from the Society of Free Radical Biology and Medicine. The review is reflective and presents our thinking that led to experiments that yielded novel observations. Critical questioning of our understanding of oxygen free radicals in biomedical problems led us to use and develop more direct and extremely sensitive methods. This included nitrone free radical spin trapping and HPLC-electrochemical detection. This technology led to the pioneering use of salicylate to trap hydroxyl free radicals and show increased flux in ischemia/reperfused brain regions and also to first sensitively detect 8-hydroxyl-2-deoxyguanosine in oxidatively damaged DNA and help assess its role in cancer development. We demonstrated that methylene blue (MB) photoinduces formation of 8-hydroxyguanine in DNA and RNA and discovered that MB sensitively photoinactivates RNA viruses, including HIV and the West Nile virus. Studies in experimental stroke led us serendipitously to discover that alpha-phenyl-tert-butylnitrone (PBN) was neuroprotective if given after the stroke. This led to extensive commercial development of NXY-059, a PBN derivative, for the treatment of stroke. More recently we discovered that PBN nitrones have potent anti-cancer activity and are active in preventing hearing loss caused by acute acoustical trauma.

Titel:	Serendipitous findings while researching oxygen free radicals.
Autor/in / Beteiligte Person:	Floyd, RA
Link:	Zum Volltext
Zeitschrift:	Free radical biology & medicine, Jg. 46 (2009-04-15), Heft 8, S. 1004
Veröffentlichung:	Tarrytown, NY : Elsevier Science ; <i>Original Publication</i>: New York : Pergamon, c1987-, 2009
Medientyp:	academicJournal
ISSN:	1873-4596 (electronic)
DOI:	10.1016/j.freeradbiomed.2009.02.003
Schlagwort:	Animals Benzenesulfonates therapeutic use Brain Neoplasms drug therapy Cyclic N-Oxides chemistry DNA Damage Guanine chemistry Guanine metabolism HIV-1 pathogenicity HIV-1 physiology Hearing Loss, Noise-Induced drug therapy Humans Methylene Blue therapeutic use RNA Virus Infections drug therapy Reactive Oxygen Species chemistry Stroke drug therapy West Nile virus pathogenicity West Nile virus physiology Guanine analogs & derivatives HIV-1 drug effects Phototherapy Reactive Oxygen Species metabolism West Nile virus drug effects
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Review Language: English [Free Radic Biol Med] 2009 Apr 15; Vol. 46 (8), pp. 1004-13. <i>Date of Electronic Publication: </i>2009 Feb 12. MeSH Terms: Phototherapy* ; Guanine / analogs & derivatives ; HIV-1 / drug effects ; Reactive Oxygen Species / metabolism ; West Nile virus / drug effects ; Animals ; Benzenesulfonates / therapeutic use ; Brain Neoplasms / drug therapy ; Cyclic N-Oxides / chemistry ; DNA Damage ; Guanine / chemistry ; Guanine / metabolism ; HIV-1 / pathogenicity ; HIV-1 / physiology ; Hearing Loss, Noise-Induced / drug therapy ; Humans ; Methylene Blue / therapeutic use ; RNA Virus Infections / drug therapy ; Reactive Oxygen Species / chemistry ; Stroke / drug therapy ; West Nile virus / pathogenicity ; West Nile virus / physiology Number of References: 78 References: Cancer Res. 1976 Apr;36(4):1510-9. (PMID: 1260768) ; Nature. 1954 Oct 9;174(4432):689-91. (PMID: 13213980) ; Neurosci Lett. 1988 May 26;88(2):233-8. (PMID: 3380359) ; Environ Health Perspect. 1991 May;92:53-62. (PMID: 1657590) ; Free Radic Biol Med. 2008 May 1;44(9):1772-84. (PMID: 18328271) ; Arch Biochem Biophys. 1989 Aug 15;273(1):106-11. (PMID: 2502945) ; Photosynth Res. 1989 Jan;22(1):11-3. (PMID: 24424674) ; Free Radic Biol Med. 1990;8(4):327-30. (PMID: 2379862) ; J Biol Chem. 1969 Nov 25;244(22):6049-55. (PMID: 5389100) ; J Gerontol. 1956 Jul;11(3):298-300. (PMID: 13332224) ; Biochim Biophys Acta. 1971 Jan 12;226(1):103-12. (PMID: 5549975) ; Ann N Y Acad Sci. 1973 Dec 31;222:1077-86. (PMID: 4361848) ; Nucleic Acids Res. 1990 Feb 11;18(3):631-5. (PMID: 2155406) ; Neurochem Res. 1989 Jul;14(7):597-605. (PMID: 2550829) ; Nucleic Acids Res. 1984 Feb 24;12(4):2137-45. (PMID: 6701097) ; Biochem Biophys Res Commun. 1986 Jun 13;137(2):841-6. (PMID: 3729941) ; Q Rev Biophys. 1980 Nov;13(4):387-564. (PMID: 7015406) ; Methods Enzymol. 1984;105:231-7. (PMID: 6328184) ; Gastroenterology. 1986 Jan;90(1):80-4. (PMID: 3753555) ; J Biol Chem. 1992 Jan 5;267(1):166-72. (PMID: 1730583) ; Basic Life Sci. 1988;49:1015-23. (PMID: 2854972) ; Arch Biochem Biophys. 1993 Feb 15;301(1):91-7. (PMID: 8382909) ; Plant Physiol. 1989 Oct;91(2):644-7. (PMID: 16667081) ; N Engl J Med. 1985 Jan 17;312(3):159-63. (PMID: 2981404) ; Arch Biochem Biophys. 1978 Jan 30;185(2):450-7. (PMID: 24414) ; Arch Biochem Biophys. 1988 Apr;262(1):266-72. (PMID: 2833169) ; Cancer Lett. 2004 Mar 31;206(1):1-13. (PMID: 15019154) ; J Free Radic Biol Med. 1986;2(1):13-8. (PMID: 3021837) ; Antiviral Res. 2004 Mar;61(3):141-51. (PMID: 15168794) ; Natl Cancer Inst Monogr. 1981 Dec;(58):123-31. (PMID: 6281648) ; Methods Enzymol. 1994;234:59-65. (PMID: 7808335) ; Science. 1954 May 7;119(3097):623-6. (PMID: 13156638) ; Toxicol Appl Pharmacol. 1997 Jan;142(1):47-55. (PMID: 9007033) ; Photochem Photobiol. 1998 Mar;67(3):350-7. (PMID: 9523535) ; Antiviral Res. 2005 Nov;68(2):84-7. (PMID: 16118025) ; Free Radic Res Commun. 1986;1(3):163-72. (PMID: 2577733) ; FASEB J. 1990 Jun;4(9):2587-97. (PMID: 2189775) ; Mol Cell Biochem. 2002 May-Jun;234-235(1-2):195-203. (PMID: 12162434) ; Biochemistry. 1983 May 24;22(11):2645-9. (PMID: 6307343) ; J Magn Reson Imaging. 2008 Sep;28(3):574-87. (PMID: 18777527) ; Cancer Res. 1991 Aug 1;51(15):3882-5. (PMID: 1855206) ; Biochem Biophys Res Commun. 1981 Apr 30;99(4):1209-15. (PMID: 7259775) ; Neurochem Res. 1985 Mar;10(3):397-410. (PMID: 4000395) ; Carcinogenesis. 1990 Sep;11(9):1447-50. (PMID: 2205403) ; Science. 1991 Dec 13;254(5038):1597. (PMID: 1684251) ; Free Radic Biol Med. 2008 Jan 1;44(1):63-72. (PMID: 18045548) ; Lancet. 1961 Jan 28;1(7170):200-1. (PMID: 13711615) ; Biochim Biophys Acta. 1983 Mar 31;756(2):204-16. (PMID: 6299374) ; J Biochem Biophys Methods. 1984 Dec;10(3-4):221-35. (PMID: 6530510) ; Arch Biochem Biophys. 1983 Aug;225(1):263-70. (PMID: 6311103) ; Arch Biochem Biophys. 2002 Jan 15;397(2):377-83. (PMID: 11795897) ; Proc Natl Acad Sci U S A. 1991 May 1;88(9):3633-6. (PMID: 1673789) ; Biophys J. 1966 Nov;6(6):825-47. (PMID: 5972381) ; EXS. 1992;62:64-72. (PMID: 1360283) ; Toxicol Appl Pharmacol. 2000 Sep 1;167(2):125-31. (PMID: 10964763) ; Photochem Photobiol. 1999 Dec;70(6):902-9. (PMID: 10628301) ; Experientia. 1977 Feb 15;33(2):197-8. (PMID: 191285) ; Free Radic Res Commun. 1991;15(3):177-86. (PMID: 1773943) ; Brain Res. 1994 May 2;644(2):267-72. (PMID: 8050038) ; Cancer Sci. 2003 Jan;94(1):26-31. (PMID: 12708470) ; Cancer Res. 1998 Oct 15;58(20):4548-51. (PMID: 9788598) ; Anal Biochem. 1990 Jul;188(1):155-8. (PMID: 2221356) ; Free Radic Biol Med. 2008 Nov 15;45(10):1361-74. (PMID: 18793715) ; J Assoc Res Otolaryngol. 2004 Mar;5(1):90-8. (PMID: 14669069) ; Biochim Biophys Acta. 1978 Mar 20;539(3):402-9. (PMID: 24480) ; Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):6866-70. (PMID: 1495976) ; Biochem Biophys Res Commun. 1977 Jan 10;74(1):79-84. (PMID: 189765) Grant Information: S10 RR016762-01A20004 United States RR NCRR NIH HHS; ES04296 United States ES NIEHS NIH HHS; R01 CA082506-05 United States CA NCI NIH HHS; AG02599 United States AG NIA NIH HHS; S10 RR016762-01A2 United States RR NCRR NIH HHS; S10 RR016762-01A20001 United States RR NCRR NIH HHS; R01 NS035747-04 United States NS NINDS NIH HHS; CA82508 United States CA NCI NIH HHS; CA082506 United States CA NCI NIH HHS; P20 RR016478 United States RR NCRR NIH HHS; S10 RR016762-01A20003 United States RR NCRR NIH HHS; GM12202 United States GM NIGMS NIH HHS; CA40228 United States CA NCI NIH HHS; R01 CA082506 United States CA NCI NIH HHS; CA42854 United States CA NCI NIH HHS; CA18591 United States CA NCI NIH HHS; R01 NS035747-05 United States NS NINDS NIH HHS; S10 RR016762 United States RR NCRR NIH HHS; S10 RR016762-01A20002 United States RR NCRR NIH HHS; R01 CA082506-01A2 United States CA NCI NIH HHS; R01 CA082506-02 United States CA NCI NIH HHS; R03 AR052041 United States AR NIAMS NIH HHS; R01 CA082506-02S1 United States CA NCI NIH HHS; NS23307 United States NS NINDS NIH HHS; GM6556 United States GM NIGMS NIH HHS; P20RR016478 United States RR NCRR NIH HHS; R01 CA082506-04 United States CA NCI NIH HHS; NS3577 United States NS NINDS NIH HHS; S10 RR016762-01A20005 United States RR NCRR NIH HHS; R01 CA082506-03 United States CA NCI NIH HHS; ES03067 United States ES NIEHS NIH HHS; CA21542 United States CA NCI NIH HHS Substance Nomenclature: 0 (Benzenesulfonates) ; 0 (Cyclic N-Oxides) ; 0 (Reactive Oxygen Species) ; 3I91332OPG (phenyl-N-tert-butylnitrone) ; 5614-64-2 (8-hydroxyguanine) ; 5Z93L87A1R (Guanine) ; 7M1J3HN9VO (disufenton sodium) ; T42P99266K (Methylene Blue) Entry Date(s): Date Created: 20090515 Date Completed: 20091113 Latest Revision: 20240416 Update Code: 20240416 PubMed Central ID: PMC2683184

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Serendipitous findings while researching oxygen free radicals.