Icariin stimulates angiogenesis by activating the MEK/ERK- and PI3K/Akt/eNOS-dependent signal pathways in human endothelial cells.

Chung, BH ; Kim, JD ; et al.

In: Biochemical and biophysical research communications, Jg. 376 (2008-11-14), Heft 2, S. 404

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We investigated the molecular effect and signal pathway of icariin, a major flavonoid of Epimedium koreanum Nakai, on angiogenesis. Icariin stimulated in vitro endothelial cell proliferation, migration, and tubulogenesis, which are typical phenomena of angiogenesis, as well as increased in vivo angiogenesis. Icariin activated the angiogenic signal modulators, ERK, phosphatidylinositol 3-kinase (PI3K), Akt, and endothelial nitric oxide synthase (eNOS), and increased NO production, without affecting VEGF expression, indicating that icariin may directly stimulate angiogenesis. Icariin-induced ERK activation and angiogenic events were significantly inhibited by the MEK inhibitor PD98059, without affecting Akt and eNOS phosphorylation. The PI3K inhibitor Wortmannin suppressed icariin-mediated angiogenesis and Akt and eNOS activation without affecting ERK phosphorylation. Moreover, the NOS inhibitor NMA partially reduced the angiogenic activity of icariin. These results suggest that icariin stimulated angiogenesis by activating the MEK/ERK- and PI3K/Akt/eNOS-dependent signal pathways and may be a useful drug for angiogenic therapy.

Titel:	Icariin stimulates angiogenesis by activating the MEK/ERK- and PI3K/Akt/eNOS-dependent signal pathways in human endothelial cells.
Autor/in / Beteiligte Person:	Chung, BH ; Kim, JD ; Kim, CK ; Kim, JH ; Won, MH ; Lee, HS ; Dong, MS ; Ha, KS ; Kwon, YG ; Kim, YM
Link:	Zum Volltext
Zeitschrift:	Biochemical and biophysical research communications, Jg. 376 (2008-11-14), Heft 2, S. 404
Veröffentlichung:	<2002- >: San Diego, CA : Elsevier ; <i>Original Publication</i>: New York, Academic Press., 2008
Medientyp:	academicJournal
ISSN:	1090-2104 (electronic)
DOI:	10.1016/j.bbrc.2008.09.001
Schlagwort:	Endothelial Cells enzymology Endothelial Cells physiology Enzyme Inhibitors pharmacology Extracellular Signal-Regulated MAP Kinases antagonists & inhibitors Extracellular Signal-Regulated MAP Kinases metabolism Humans MAP Kinase Kinase Kinases antagonists & inhibitors MAP Kinase Kinase Kinases metabolism Nitric Oxide Synthase Type III antagonists & inhibitors Nitric Oxide Synthase Type III metabolism Phosphatidylinositol 3-Kinases metabolism Phosphoinositide-3 Kinase Inhibitors Proto-Oncogene Proteins c-akt antagonists & inhibitors Proto-Oncogene Proteins c-akt metabolism Angiogenesis Inducing Agents pharmacology Endothelial Cells drug effects Flavonoids pharmacology MAP Kinase Signaling System drug effects Neovascularization, Physiologic drug effects
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Biochem Biophys Res Commun] 2008 Nov 14; Vol. 376 (2), pp. 404-8. <i>Date of Electronic Publication: </i>2008 Sep 16. MeSH Terms: Angiogenesis Inducing Agents / pharmacology ; Endothelial Cells / drug effects ; Flavonoids / pharmacology ; MAP Kinase Signaling System / drug effects ; Neovascularization, Physiologic / *drug effects ; Endothelial Cells / enzymology ; Endothelial Cells / physiology ; Enzyme Inhibitors / pharmacology ; Extracellular Signal-Regulated MAP Kinases / antagonists & inhibitors ; Extracellular Signal-Regulated MAP Kinases / metabolism ; Humans ; MAP Kinase Kinase Kinases / antagonists & inhibitors ; MAP Kinase Kinase Kinases / metabolism ; Nitric Oxide Synthase Type III / antagonists & inhibitors ; Nitric Oxide Synthase Type III / metabolism ; Phosphatidylinositol 3-Kinases / metabolism ; Phosphoinositide-3 Kinase Inhibitors ; Proto-Oncogene Proteins c-akt / antagonists & inhibitors ; Proto-Oncogene Proteins c-akt / metabolism Comments: Erratum in: Biochem Biophys Res Commun. 2009 Jun 12;383(4):522. Kim, Jung Huan [corrected to Kim, Jung Hwan]. Substance Nomenclature: 0 (Angiogenesis Inducing Agents) ; 0 (Enzyme Inhibitors) ; 0 (Flavonoids) ; 0 (Phosphoinositide-3 Kinase Inhibitors) ; EC 1.14.13.39 (NOS3 protein, human) ; EC 1.14.13.39 (Nitric Oxide Synthase Type III) ; EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) ; EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases) ; EC 2.7.11.25 (MAP Kinase Kinase Kinases) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20080916 Date Completed: 20081107 Latest Revision: 20191210 Update Code: 20231215

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