Simultaneous quantitation of five flavonoid glycosides in Herba Epimedii by high-performance liquid chromatography-tandem mass spectrometry.
In: Phytochemical analysis : PCA, Jg. 19 (2008), Heft 1, S. 71-7
Online
academicJournal
Zugriff:
A rapid and accurate reversed-phase liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method has been developed and validated for the quantitative determination of five flavonoid glycosides, icariin, epimedin A, epimedin B, epimedin C and hyperin in Herba Epimedii. Chromatographic separations were performed using a C(18) narrow-bore HPLC column; a mixture of an aqueous solution of ammonium formate (pH 4.0) and acetonitrile was used as the mobile phase, with compounds detected in the positive ion mode with multiple-reaction monitoring using a triple-quadrupole mass spectrometer equipped with an electrospray ionisation interface. This method for the determination of the reported flavonoid glycosides was accurate and reproducible, with a lower limit of quantication of 0.5 microg/mL. The standard calibration curves for the above-mentioned compounds were linear (r(2) > 0.998) over the concentration range 0.5-10.0 microg/mL. The relative standard deviations for intra- and inter-day precision over the concentration range for the flavonoid glycosides were lower than 7.8% with accuracy between 90.1 and 111.0%. The established method was successfully applied to the quality assessment of samples of Herba Epimedii collected from Korea and China.
((c) 2007 John Wiley & Sons, Ltd.)
Titel: |
Simultaneous quantitation of five flavonoid glycosides in Herba Epimedii by high-performance liquid chromatography-tandem mass spectrometry.
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Autor/in / Beteiligte Person: | Islam, NM ; Yoo, HH ; Lee, MW ; Dong, MS ; Park, YI ; Jeong, HS ; Kim, DH |
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Zeitschrift: | Phytochemical analysis : PCA, Jg. 19 (2008), Heft 1, S. 71-7 |
Veröffentlichung: | Chichester, Sussex, UK : Wiley, c1990-, 2008 |
Medientyp: | academicJournal |
ISSN: | 0958-0344 (print) |
DOI: | 10.1002/pca.1018 |
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