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Purpose: To evaluate the cysticidal effect of polyhexamethylene biguanide (PHMB) and chlorhexidine on Acanthamoeba and its toxic effect on cultured human keratocytes.

Methods: Each well of a twofold-diluted Acanthamoeba cyst-containing suspension of 5 x 10(4) cysts/mL was treated with PHMB and chlorhexidine for 8, 24, and 48 hours to determine the minimal cysticidal concentration (MCC) of each disinfectant. Human corneal keratocytes (5 x 10(4) cells/mL) were exposed to PHMB and chlorhexidine for the same time to determine the survival rate of keratocytes. Inverted phase-contrast and electron microscopy were used to observe the morphologic changes.

Results: The mean MCC of PHMB for 8, 24, and 48 hours was 9.42, 5.62, and 2.37 microg/mL, respectively. The mean MCC of chlorhexidine for 8, 24, and 48 hours was 24.32, 10.02, and 7.02 microg/mL, respectively. The respective survival rate of keratocytes at the MCC was 91.7%, 64.6%, and 49.7% for PHMB and 95.7%, 90.6%, and 78.1% for chlorhexidine, respectively. The cysts and keratocytes showed more damaged appearances after treatment with PHMB than chlorhexidine.

Conclusions: PHMB and chlorhexidine showed a similar amoebicidal efficacy. However, PHMB seemed to be more toxic to keratocytes than chlorhexidine.

Titel:	Cysticidal effect on acanthamoeba and toxicity on human keratocytes by polyhexamethylene biguanide and chlorhexidine.
Autor/in / Beteiligte Person:	Lee, JE ; Oum, BS ; Choi, HY ; Yu, HS ; Lee, JS
Zeitschrift:	Cornea, Jg. 26 (2007-07-01), Heft 6, S. 736
Veröffentlichung:	Hagerstown, MD : Lippincott Williams & Wilkins ; <i>Original Publication</i>: New York, N.Y. : Masson Pub. USA, c1982-, 2007
Medientyp:	academicJournal
ISSN:	0277-3740 (print)
DOI:	10.1097/ICO.0b013e31805b7e8e
Schlagwort:	Acanthamoeba castellanii ultrastructure Animals Cell Survival Cells, Cultured Corneal Stroma ultrastructure Fibroblasts drug effects Fibroblasts ultrastructure Humans Microscopy, Electron, Transmission Microscopy, Phase-Contrast Parasitic Sensitivity Tests Acanthamoeba castellanii drug effects Amebicides toxicity Biguanides toxicity Chlorhexidine toxicity Corneal Stroma drug effects Disinfectants toxicity
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Cornea] 2007 Jul; Vol. 26 (6), pp. 736-41. MeSH Terms: Acanthamoeba castellanii / drug effects ; Amebicides / toxicity ; Biguanides / toxicity ; Chlorhexidine / toxicity ; Corneal Stroma / drug effects ; Disinfectants / toxicity ; Acanthamoeba castellanii / ultrastructure ; Animals ; Cell Survival ; Cells, Cultured ; Corneal Stroma / ultrastructure ; Fibroblasts / drug effects ; Fibroblasts / ultrastructure ; Humans ; Microscopy, Electron, Transmission ; Microscopy, Phase-Contrast ; Parasitic Sensitivity Tests Substance Nomenclature: 0 (Amebicides) ; 0 (Biguanides) ; 0 (Disinfectants) ; 322U039GMF (polihexanide) ; R4KO0DY52L (Chlorhexidine) Entry Date(s): Date Created: 20070627 Date Completed: 20071001 Latest Revision: 20220408 Update Code: 20231215

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Cysticidal effect on acanthamoeba and toxicity on human keratocytes by polyhexamethylene biguanide and chlorhexidine.