Luman, a new partner of HIV-1 TMgp41, interferes with Tat-mediated transcription of the HIV-1 LTR.
In: Journal of molecular biology, Jg. 364 (2006-12-15), Heft 5, S. 1034
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Zugriff:
In our search for new partners of the HIV-1 envelope glycoprotein (Env), we found that the cytoplasmic domain of the TMgp41 (TMgp41 CD) subunit of HIV-1 Env interacted with Luman, a transcription factor of the CREB/ATF family. Luman is anchored in the endoplasmic reticulum membrane and subjected to activation by regulated intramembrane proteolysis (RIP). The RIP process permits the release of the activated amino-terminal fragment of Luman into the cytoplasm, and its import into the nucleus. Here, we demonstrate that interaction between the TMgp41 CD and Luman requires a region encompassing the b-Zip and TM domains of Luman and decreases the stability of this factor. Moreover, we found that overexpression of a constitutively active form of Luman in cells transfected with HXB2R HIV-1 provirus decreased the intracellular expression of Gag and Env and led to a decrease in virion release. This negative effect of activated Luman on HIV-1 production was correlated to the inhibition of Tat transactivation of the HIV-1 LTR, which might be related to an interaction of activated Luman with Tat. Altogether, these results show that Luman acts as a partner of two major HIV-1 proteins: the TMgp41 Env subunit and Tat. The interaction between the TMgp41 subunit of Env and Luman affects the stability of the full-length Luman protein, the precursor of the activated, nuclear form of Luman, which acts negatively on Tat-mediated HIV-1 transactivation.
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Luman, a new partner of HIV-1 TMgp41, interferes with Tat-mediated transcription of the HIV-1 LTR.
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Autor/in / Beteiligte Person: | Blot, G ; Lopez-Vergès, S ; Treand, C ; Kubat, NJ ; Delcroix-Genête, D ; Emiliani, S ; Benarous, R ; Berlioz-Torrent, C |
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Zeitschrift: | Journal of molecular biology, Jg. 364 (2006-12-15), Heft 5, S. 1034 |
Veröffentlichung: | Amsterdam : Elsevier ; <i>Original Publication</i>: 1959- : London : Academic Press, 2006 |
Medientyp: | academicJournal |
ISSN: | 0022-2836 (print) |
DOI: | 10.1016/j.jmb.2006.09.080 |
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