Einzeltreffer — DigiBib

Background and Purpose: As pituitary adenylate cyclase-activating polypeptide 38 (PACAP 38)- and vasoactive intestinal peptide (VIP) are widely distributed in the urinary tract, the current study investigated the receptors and mechanisms involved in relaxations induced by these peptides in the pig bladder neck.

Experimental Approach: Urothelium-denuded strips were suspended in organ baths for isometric force recordings and the relaxations to VIP and PACAP analogues were investigated.

Key Results: VIP, PACAP 38, PACAP 27 and [Ala(11,22,28)]-VIP produced similar relaxations. Inhibition of neuronal voltage-gated Ca(2+) channels reduced relaxations to PACAP 38 and increased those induced by VIP. Blockade of capsaicin-sensitive primary afferents (CSPA), nitric oxide (NO)-synthase or guanylate cyclase reduced the PACAP 38 relaxations but failed to modify the VIP responses. Inhibition of VIP/PACAP receptors and of voltage-gated K(+) channels reduced PACAP 38 and VIP relaxations, which were not modified by the K(+) channel blockers iberiotoxin, charybdotoxin, apamin or glibenclamide. The phosphodiesterase 4 inhibitor rolipram and the adenylate cyclase activator forskolin produced potent relaxations. Blockade of protein kinase A (PKA) reduced PACAP 38- and VIP-induced relaxations.

Conclusions and Implications: PACAP 38 and VIP relax the pig urinary bladder neck through muscle VPAC(2) receptors linked to the cAMP-PKA pathway and involve activation of voltage-gated K(+) channels. Facilitatory PAC(1) receptors located at CSPA and coupled to NO release, and inhibitory VPAC receptors at motor endings are also involved in the relaxations to PACAP 38 and VIP, respectively. VIP/PACAP receptor antagonists could be useful in the therapy of urinary incontinence produced by intrinsic sphincter deficiency.

Titel:	Neuronal and smooth muscle receptors involved in the PACAP- and VIP-induced relaxations of the pig urinary bladder neck.
Autor/in / Beteiligte Person:	Hernández, M ; Barahona, MV ; Recio, P ; Benedito, S ; Martínez, AC ; Rivera, L ; García-Sacristán, A ; Prieto, D ; Orensanz, LM
Link:	Zum Volltext
Zeitschrift:	British journal of pharmacology, Jg. 149 (2006-09-01), Heft 1, S. 100-9
Veröffentlichung:	London : Wiley ; <i>Original Publication</i>: London, Macmillian Journals Ltd., 2006
Medientyp:	academicJournal
ISSN:	0007-1188 (print)
DOI:	10.1038/sj.bjp.0706832
Schlagwort:	Adenylyl Cyclases physiology Animals Female In Vitro Techniques Male Muscle Contraction drug effects Phosphodiesterase Inhibitors pharmacology Potassium Channels drug effects Rolipram pharmacology Signal Transduction physiology Swine Urinary Bladder innervation Urothelium physiology Muscle, Smooth, Vascular drug effects Neurons drug effects Pituitary Adenylate Cyclase-Activating Polypeptide pharmacology Urinary Bladder drug effects Vasoactive Intestinal Peptide pharmacology Vasodilator Agents pharmacology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Br J Pharmacol] 2006 Sep; Vol. 149 (1), pp. 100-9. <i>Date of Electronic Publication: </i>2006 Jul 17. MeSH Terms: Muscle, Smooth, Vascular / drug effects ; Neurons / drug effects ; Pituitary Adenylate Cyclase-Activating Polypeptide / pharmacology ; Urinary Bladder / drug effects ; Vasoactive Intestinal Peptide / pharmacology ; Vasodilator Agents / pharmacology ; Adenylyl Cyclases / physiology ; Animals ; Female ; In Vitro Techniques ; Male ; Muscle Contraction / drug effects ; Phosphodiesterase Inhibitors / pharmacology ; Potassium Channels / drug effects ; Rolipram / pharmacology ; Signal Transduction / physiology ; Swine ; Urinary Bladder / innervation ; Urothelium / physiology References: Regul Pept. 2002 Jul 15;107(1-3):115-23. (PMID: 12137973) ; Am J Physiol Cell Physiol. 2002 Mar;282(3):C508-17. (PMID: 11832336) ; J Mol Neurosci. 1998 Aug;11(1):43-56. (PMID: 9826785) ; Trends Pharmacol Sci. 1999 Aug;20(8):324-9. (PMID: 10431211) ; Kidney Int. 2003 Oct;64(4):1356-64. (PMID: 12969154) ; Br J Pharmacol. 2004 Jan;141(1):123-31. (PMID: 14662737) ; J Urol. 1981 Jun;125(6):830-4. (PMID: 7241684) ; Am J Physiol. 1992 Apr;262(4 Pt 1):G774-8. (PMID: 1566853) ; Nihon Hinyokika Gakkai Zasshi. 1993 Mar;84(3):440-9. (PMID: 8099983) ; Am J Physiol. 1993 Sep;265(3 Pt 2):R537-43. (PMID: 8214143) ; J Biol Chem. 1994 Jun 10;269(23):15977-80. (PMID: 7515875) ; J Urol. 1994 Oct;152(4):1292-6. (PMID: 7520952) ; Endocrinology. 1994 Dec;135(6):2662-80. (PMID: 7988457) ; Urology. 1995 May;45(5):893-901. (PMID: 7747383) ; Neurosci Lett. 1995 Feb 15;186(1):33-6. (PMID: 7783945) ; Neurourol Urodyn. 1996;15(1):59-70. (PMID: 8696357) ; Br J Pharmacol. 1996 Oct;119(4):623-30. (PMID: 8904634) ; J Anat. 1997 Jan;190 ( Pt 1):125-34. (PMID: 9034888) ; Br J Pharmacol. 1997 Feb;120(4):609-16. (PMID: 9051298) ; J Vasc Res. 1997 Jan-Feb;34(1):11-8. (PMID: 9075821) ; Biochim Biophys Acta. 1997 Mar 27;1356(1):64-70. (PMID: 9099992) ; Cell Signal. 1997 May-Jun;9(3-4):269-76. (PMID: 9218127) ; Regul Pept. 1997 Apr 30;69(3):155-65. (PMID: 9226400) ; Br J Pharmacol. 1997 Aug;121(8):1605-12. (PMID: 9283693) ; Eur J Pharmacol. 1997 Sep 3;334(1):61-6. (PMID: 9346329) ; Neuroscience. 1998 Apr;83(4):1261-72. (PMID: 9502264) ; J Neurochem. 1998 Apr;70(4):1652-61. (PMID: 9523583) ; Pharmacol Rev. 1998 Jun;50(2):265-70. (PMID: 9647867) ; Regul Pept. 1999 Dec 23;85(2-3):87-92. (PMID: 10651061) ; Pharmacol Rev. 2000 Jun;52(2):269-324. (PMID: 10835102) ; J Biol Chem. 2000 Aug 4;275(31):24003-12. (PMID: 10801840) ; J Urol. 2000 Oct;164(4):1371-5. (PMID: 10992417) ; Neurourol Urodyn. 2001;20(3):297-308. (PMID: 11385696) ; Urol Res. 2001 Dec;29(6):388-92. (PMID: 11828991) ; World J Urol. 2002 Sep;20(4):244-9. (PMID: 12215855) Substance Nomenclature: 0 (Phosphodiesterase Inhibitors) ; 0 (Pituitary Adenylate Cyclase-Activating Polypeptide) ; 0 (Potassium Channels) ; 0 (Vasodilator Agents) ; 37221-79-7 (Vasoactive Intestinal Peptide) ; EC 4.6.1.1 (Adenylyl Cyclases) ; K676NL63N7 (Rolipram) Entry Date(s): Date Created: 20060719 Date Completed: 20061024 Latest Revision: 20181113 Update Code: 20240513 PubMed Central ID: PMC1629402

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Neuronal and smooth muscle receptors involved in the PACAP- and VIP-induced relaxations of the pig urinary bladder neck.