Molecular cloning and functional expression of d-arabitol dehydrogenase gene from Gluconobacter oxydans in Escherichia coli.
In: FEMS microbiology letters, Jg. 252 (2005-11-01), Heft 1, S. 35-42
Online
academicJournal
Zugriff:
A NADP-dependent d-arabitol dehydrogenase gene was cloned from Gluconobacter oxydans CGMCC 1.110 and functionally expressed in Escherichia coli. With d-arabitol as sole carbon source, E. coli transformants grew rapidly in minimal medium, and produced d-xylulose. The enzymatic properties of the 29kDa enzyme were documented. The DNA sequence surrounding the gene suggested that it is part of an operon with several components of a sugar alcohol transporter system, and the d-arabitol dehydrogenase gene belongs to the short-chain dehydrogenase family.
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Molecular cloning and functional expression of d-arabitol dehydrogenase gene from Gluconobacter oxydans in Escherichia coli.
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Autor/in / Beteiligte Person: | Cheng, H ; Jiang, N ; Shen, A ; Feng, Y |
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Zeitschrift: | FEMS microbiology letters, Jg. 252 (2005-11-01), Heft 1, S. 35-42 |
Veröffentlichung: | 2015- : Oxford Oxford University Press ; <i>Original Publication</i>: Amsterdam, Published by Elsevier/North Holland on behalf of the Federation of European Microbiological Societies., 2005 |
Medientyp: | academicJournal |
ISSN: | 0378-1097 (print) |
DOI: | 10.1016/j.femsle.2005.08.023 |
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