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We examined the effect of the inflammatory mediator interleukin-1alpha (IL-1alpha) on cell proliferation, alkaline phosphatase (ALPase) activity, and the expressions of cartilage matrix proteins, bone morphogenetic protein-2 (BMP-2), and BMP-2 receptors in human chondrosarcoma cell line OUMS-27 (chondrocytes). The cells were cultured with Dulbecco's modified Eagle's medium containing 15% fetal bovine serum with 0, 1, 10, or 100 units/ml of IL-1alpha for up to 14 days. The expressions of cartilage matrix proteins, BMP-2, and BMP-2 receptors were estimated by determining mRNA levels using semiquantitative or real-time PCR and/or by determining protein levels using Enzyme-linked immunosorbent assay. Cell proliferation was decreased after 5 days in culture with IL-1alpha. The ALPase activity was decreased significantly in the presence of IL-1alpha until day 10 of culture. The expression of type II collagen was significantly decreased after 7 days in culture with IL-1alpha. The expressions of aggrecan and link protein were significantly decreased through day 14 of culture with IL-1alpha. The expression of BMP-2 was increased at days 3, 7, and 14 of culture with IL-1alpha, while the expression of type II receptor for BMP-2 was significantly decreased in the samples. These results suggest that IL-1alpha suppresses the expression of cartilage matrix proteins through a suppression of the autocrine action of BMP-2, brought about by the decrease in BMP-2 receptor expression in chondrocytes.

Titel:	Effect of IL-1alpha on the expression of cartilage matrix proteins in human chondrosarcoma cell line OUMS-27.
Autor/in / Beteiligte Person:	Aida, Y ; Maeno, M ; Ito-Kato, E ; Suzuki, N ; Shiratsuchi, H ; Matsumura, H
Link:	Zum Volltext
Zeitschrift:	Life sciences, Jg. 75 (2004-11-12), Heft 26, S. 3173
Veröffentlichung:	<2008->: Amsterdam : Elsevier ; <i>Original Publication</i>: Oxford; Elmsford, N. Y. [etc.] Pergamon Press., 2004
Medientyp:	academicJournal
ISSN:	0024-3205 (print)
DOI:	10.1016/j.lfs.2004.06.011
Schlagwort:	Alkaline Phosphatase metabolism Bone Morphogenetic Protein Receptors Bone Morphogenetic Proteins metabolism Cartilage Oligomeric Matrix Protein Collagen Type II metabolism DNA Primers Enzyme-Linked Immunosorbent Assay Humans Matrilin Proteins Receptors, Growth Factor metabolism Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured Cell Proliferation drug effects Extracellular Matrix Proteins metabolism Gene Expression drug effects Glycoproteins metabolism Interleukin-1 pharmacology RNA, Messenger metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Language: English [Life Sci] 2004 Nov 12; Vol. 75 (26), pp. 3173-84. MeSH Terms: Cell Proliferation / drug effects ; Extracellular Matrix Proteins / metabolism ; Gene Expression / drug effects ; Glycoproteins / metabolism ; Interleukin-1 / pharmacology ; RNA, Messenger / metabolism ; Alkaline Phosphatase / metabolism ; Bone Morphogenetic Protein Receptors ; Bone Morphogenetic Proteins / metabolism ; Cartilage Oligomeric Matrix Protein ; Collagen Type II / metabolism ; DNA Primers ; Enzyme-Linked Immunosorbent Assay ; Humans ; Matrilin Proteins ; Receptors, Growth Factor / metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Tumor Cells, Cultured Substance Nomenclature: 0 (Bone Morphogenetic Proteins) ; 0 (Cartilage Oligomeric Matrix Protein) ; 0 (Collagen Type II) ; 0 (DNA Primers) ; 0 (Extracellular Matrix Proteins) ; 0 (Glycoproteins) ; 0 (Interleukin-1) ; 0 (Matrilin Proteins) ; 0 (RNA, Messenger) ; 0 (Receptors, Growth Factor) ; 0 (TSP5 protein, human) ; EC 2.7.11.30 (Bone Morphogenetic Protein Receptors) ; EC 3.1.3.1 (Alkaline Phosphatase) Entry Date(s): Date Created: 20041019 Date Completed: 20050103 Latest Revision: 20131121 Update Code: 20231215

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Effect of IL-1alpha on the expression of cartilage matrix proteins in human chondrosarcoma cell line OUMS-27.