Cloning vectors derived from Streptomyces niveus plasmid pSN2.
In: Letters in applied microbiology, Jg. 11 (1990-11-01), Heft 5, S. 240
academicJournal
Zugriff:
Two high copy number, broad host range, general purpose cloning vectors, pLG5 and pLG10, derived from the unstable Streptomyces niveus plasmid pSN2 are described. pLG5 (5.5 kb) and pLG10 (6.5 kb) both carry the thiostrepton resistance (TsrR) and lethal zygosis (Ltz+) markers and have single cloning sites within a non-essential region and the tsr gene. pLG505 (7.4 kb) was constructed by cloning the viomycin resistance (vph) gene into the single BamHI site of pLG5 to give a further vector with insertion and replacement sites which inactivate either the TsrR or VioR functions.
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Cloning vectors derived from Streptomyces niveus plasmid pSN2.
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Autor/in / Beteiligte Person: | Hussain, HA ; Ritchie, DA |
Zeitschrift: | Letters in applied microbiology, Jg. 11 (1990-11-01), Heft 5, S. 240 |
Veröffentlichung: | 2023- : Oxford : Oxford University Press ; <i>Original Publication</i>: Oxford, UK : Published for the Society for Applied Bacteriology by Blackwell Scientific Publications, [c1985-, 1990 |
Medientyp: | academicJournal |
ISSN: | 0266-8254 (print) |
DOI: | 10.1111/j.1472-765x.1990.tb00171.x |
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