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Pseudomonas aeruginosa clinical cystic fibrosis isolate CHA was mutagenized with Tn5Tc to identify new genes involved in type III secretion system (TTSS)-dependent cytotoxicity toward human polymorphonuclear neutrophils. Among 25 mutants affected in TTSS function, 14 contained the insertion at different positions in the aceAB operon encoding the PDH-E1 and -E2 subunits of pyruvate dehydrogenase. In PDH mutants, no transcriptional activation of TTSS genes in response to calcium depletion occurred. Expression in trans of ExsA restored TTSS function and cytotoxicity.

Titel:	Activation of the Pseudomonas aeruginosa type III secretion system requires an intact pyruvate dehydrogenase aceAB operon.
Autor/in / Beteiligte Person:	Dacheux, D ; Epaulard, O ; de Groot A ; Guery, B ; Leberre, R ; Attree, I ; Polack, B ; Toussaint, B
Link:	Zum Volltext
Zeitschrift:	Infection and immunity, Jg. 70 (2002-07-01), Heft 7, S. 3973
Veröffentlichung:	Washington, DC : American Society For Microbiology ; <i>Original Publication</i>: [Bethesda, Md.] American Society for Microbiology., 2002
Medientyp:	academicJournal
ISSN:	0019-9567 (print)
DOI:	10.1128/IAI.70.7.3973-3977.2002
Schlagwort:	Animals Bacterial Proteins metabolism Cystic Fibrosis microbiology DNA-Binding Proteins genetics DNA-Binding Proteins physiology Dihydrolipoyllysine-Residue Acetyltransferase Disease Models, Animal Histidine Kinase Humans L-Lactate Dehydrogenase metabolism Mutagenesis, Insertional Neutrophils microbiology Phosphoprotein Phosphatases genetics Phosphoprotein Phosphatases metabolism Pneumonia, Bacterial microbiology Pore Forming Cytotoxic Proteins Protein Kinases metabolism Pseudomonas Infections microbiology Pseudomonas aeruginosa genetics Pseudomonas aeruginosa isolation & purification Pseudomonas aeruginosa pathogenicity Pyruvate Dehydrogenase Complex genetics Rats Rats, Sprague-Dawley Trans-Activators genetics Trans-Activators physiology Operon physiology Pseudomonas aeruginosa enzymology Pyruvate Dehydrogenase Complex physiology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Infect Immun] 2002 Jul; Vol. 70 (7), pp. 3973-7. MeSH Terms: Operon / physiology ; Pseudomonas aeruginosa / enzymology ; Pyruvate Dehydrogenase Complex / *physiology ; Animals ; Bacterial Proteins / metabolism ; Cystic Fibrosis / microbiology ; DNA-Binding Proteins / genetics ; DNA-Binding Proteins / physiology ; Dihydrolipoyllysine-Residue Acetyltransferase ; Disease Models, Animal ; Histidine Kinase ; Humans ; L-Lactate Dehydrogenase / metabolism ; Mutagenesis, Insertional ; Neutrophils / microbiology ; Phosphoprotein Phosphatases / genetics ; Phosphoprotein Phosphatases / metabolism ; Pneumonia, Bacterial / microbiology ; Pore Forming Cytotoxic Proteins ; Protein Kinases / metabolism ; Pseudomonas Infections / microbiology ; Pseudomonas aeruginosa / genetics ; Pseudomonas aeruginosa / isolation & purification ; Pseudomonas aeruginosa / pathogenicity ; Pyruvate Dehydrogenase Complex / genetics ; Rats ; Rats, Sprague-Dawley ; Trans-Activators / genetics ; Trans-Activators / physiology References: Infect Immun. 1999 Nov;67(11):6164-7. (PMID: 10531282) ; J Biol Chem. 1956 Jan;218(1):97-106. (PMID: 13278318) ; Microbes Infect. 1999 Oct;1(12):1005-14. (PMID: 10617932) ; Infect Immun. 2000 May;68(5):2916-24. (PMID: 10768989) ; Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4885-90. (PMID: 10758151) ; FEMS Microbiol Rev. 2000 Jul;24(3):303-16. (PMID: 10841975) ; Nature. 2000 Aug 31;406(6799):959-64. (PMID: 10984043) ; Infect Immun. 2001 Jan;69(1):538-42. (PMID: 11119548) ; Mol Microbiol. 2001 Apr;40(1):76-85. (PMID: 11298277) ; Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648-52. (PMID: 377280) ; J Gen Microbiol. 1980 Oct;120(2):385-92. (PMID: 6785385) ; J Bacteriol. 1990 Nov;172(11):6568-72. (PMID: 2172217) ; Microbiology. 1996 Oct;142 ( Pt 10):2785-93. (PMID: 8885394) ; Mol Microbiol. 1996 Dec;22(5):991-1003. (PMID: 8971719) ; Mol Microbiol. 1997 Nov;26(4):621-9. (PMID: 9427393) ; Microbiol Mol Biol Rev. 1998 Jun;62(2):379-433. (PMID: 9618447) ; J Biol Chem. 1999 Mar 19;274(12):7901-6. (PMID: 10075684) ; Nat Med. 1999 Apr;5(4):392-8. (PMID: 10202927) ; Appl Environ Microbiol. 1999 May;65(5):2065-71. (PMID: 10224002) ; Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12389-93. (PMID: 10535932) Substance Nomenclature: 0 (Bacterial Proteins) ; 0 (DNA-Binding Proteins) ; 0 (ExsA protein, bacteria) ; 0 (PopB protein, Ralstonia solanacearum) ; 0 (Pore Forming Cytotoxic Proteins) ; 0 (Pyruvate Dehydrogenase Complex) ; 0 (Trans-Activators) ; 0 (pyruvate dehydrogenase multienzyme complex E1) ; EC 1.1.1.27 (L-Lactate Dehydrogenase) ; EC 2.3.1.12 (Dihydrolipoyllysine-Residue Acetyltransferase) ; EC 2.7.- (Protein Kinases) ; EC 2.7.13.1 (Histidine Kinase) ; EC 3.1.3.16 (Phosphoprotein Phosphatases) ; EC 3.1.3.16 (protein phosphatase 4) Entry Date(s): Date Created: 20020618 Date Completed: 20020730 Latest Revision: 20220708 Update Code: 20240513 PubMed Central ID: PMC128050

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Activation of the Pseudomonas aeruginosa type III secretion system requires an intact pyruvate dehydrogenase aceAB operon.