Updated molecular epidemiology of carbapenem-non-susceptible Escherichia coli in Taiwan: first identification of KPC-2 or NDM-1-producing E. coli in Taiwan.
In: BMC Infectious Diseases, Jg. 13 (2013-01-10), Heft 1, S. 1-17
Online
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Zugriff:
Background The global spread and increasing incidence of carbapenem-resistant Enterobacteriaceae have resulted in treatment and public health concerns. Here, we present an investigation of the molecular mechanisms and clonality of carbapenem-non-susceptible Escherichia coli (CnSEC) based on a nationwide survey in Taiwan. Methods We collected 32 and 43 carbapenem-non-susceptible E. coli isolates in 2010 and 2012, respectively. The genes encoding cabapenemases and plasmidic AmpC-type and extendedspectrum ß-lactamases (EBSLs) were analyzed by polymerase chain reaction (PCR). The major porin channels OmpF and OmpC were evaluated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE). Molecular typing was performed with pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Results The resistance rates of CnSEC isolates to cefazolin, cefotaxime, cefoxitin, ceftazidime, and ertapenem were all 100%, and most (94.7%) isolates were CMY producers. The main mechanism of CnSEC in Taiwan is via plasmidic AmpC ß-lactamase CMY-2 and DHA-1 in combination with the loss of OmpC/F. In 2010, one isolate was confirmed to harbor blaIMP-8; a KPC-2 producer and an NDM-1 producer were detected in 2012. No isolate had VIM- or OXA-carbapenemases. ST131 was the predominant ST type (33.3%). PFGE revealed no large cluster in CnSEC isolates in Taiwan. Conclusion The co-existence of plasmidic AmpC ß-lactamase and outer membrane protein loss is the main mechanism for CnSEC in Taiwan. The emergence of KPC-2 and NDM-1 in 2012 and the predominance of ST131 warrant close monitoring and infection control. [ABSTRACT FROM AUTHOR]
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Updated molecular epidemiology of carbapenem-non-susceptible Escherichia coli in Taiwan: first identification of KPC-2 or NDM-1-producing E. coli in Taiwan.
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Autor/in / Beteiligte Person: | Ma, Ling ; L Kristopher Siu ; Lin, Jung-Chung ; Wu, Tsu-Lan ; Fung, Chang-Phone ; Wang, Jann-Tay ; Lu, Po-Liang ; Chuang, Yin-Ching |
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Zeitschrift: | BMC Infectious Diseases, Jg. 13 (2013-01-10), Heft 1, S. 1-17 |
Veröffentlichung: | 2013 |
Medientyp: | academicJournal |
ISSN: | 1471-2334 (print) |
DOI: | 10.1186/1471-2334-13-599 |
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