The bifunctional aldehyde–alcohol dehydrogenase controls ethanol and acetate production in Entamoeba histolytica under aerobic conditions
In: FEBS Letters, Jg. 587 (2013-01-16), Heft 2, S. 178-184
Online
academicJournal
Zugriff:
Abstract: By applying metabolic control analysis and inhibitor titration we determined the degree of control (flux control coefficient) of pyruvate:ferredoxin oxidoreductase (PFOR) and bifunctional aldehyde–alcohol dehydrogenase (ADHE) over the fluxes of fermentative glycolysis of Entamoeba histolytica subjected to aerobic conditions. The flux-control coefficients towards ethanol and acetate formation determined for PFOR titrated with diphenyleneiodonium were 0.07 and 0.09, whereas for ADHE titrated with disulfiram were 0.33 and −0.19, respectively. ADHE inhibition induced significant accumulation of glycolytic intermediates and lower ATP content. These results indicate that ADHE exerts significant flux-control on the carbon end-product formation of amoebas subjected to aerobic conditions. [Copyright &y& Elsevier]
Titel: |
The bifunctional aldehyde–alcohol dehydrogenase controls ethanol and acetate production in Entamoeba histolytica under aerobic conditions
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Autor/in / Beteiligte Person: | Pineda, Erika ; Encalada, Rusely ; Olivos-García, Alfonso ; Néquiz, Mario ; Moreno-Sánchez, Rafael ; Saavedra, Emma |
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Zeitschrift: | FEBS Letters, Jg. 587 (2013-01-16), Heft 2, S. 178-184 |
Veröffentlichung: | 2013 |
Medientyp: | academicJournal |
ISSN: | 0014-5793 (print) |
DOI: | 10.1016/j.febslet.2012.11.020 |
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